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[热点] 国自然申请面上模板最新2026版出了吗? lice_1987 2026-01-18 刚刚
[DNA重组] [已完结]GST融合蛋白的分子量是多大呀 ?    ( 1 2 3 4 ) (评阅+1) (34/13923) yuguiyan 2016-08-01 2016-08-05 08:24:16 by yuguiyan
[DNA重组] [已完结]双酶切 胶回收 (评阅+1) (8/2370) Yuanfang_818 2016-07-31 2016-08-03 13:21:55 by feierorange
[DNA重组] [已完结]真核表达载体和宿主如何选择 (评阅+1) (6/1594) wenyi2015 2016-07-31 2016-08-02 15:45:24 by wenyi2015
[DNA重组] [已完结]IPTG诱导后,采用反复冻融的方法提取出蛋白 (评阅+1) (7/3238) yuguiyan 2016-07-27 2016-07-31 15:35:50 by yuguiyan
[DNA重组] [已完结]GST融合蛋白纯化时,样品制备问题    ( 1 2 ) (15/983) yuguiyan 2016-07-27 2016-07-31 08:05:39 by cm14704019
[DNA重组] 阳性克隆测序 (评阅+1) (2/1164) hnnydx 2016-07-29 2016-07-31 00:03:11 by 小冀-
[DNA重组] [已完结]酶切后的载体怎样去磷酸化? (评阅+1) (2/2407) 小李纸11 2016-07-27 2016-07-30 14:15:50 by Eternity宇
[DNA重组] [已完结]两片段连接 (评阅+1) (0/352) 樱花花开 2016-07-27 2016-07-27 15:50:18 by 樱花花开
[DNA重组] [已完结]两个小片段与载体连接    ( 1 2 ) (16/2837) 荷岚102 2016-05-03 2016-07-27 12:37:42 by 樱花花开
[DNA重组] [已完结]做转化,涂平板的时候,可以不用超净工作台?    ( 1 2 ) (评阅+1) (15/3081) 晴雪2013 2016-07-14 2016-07-26 15:58:50 by 阿尔卑斯英
[DNA重组] 可以同时将2个目的片段克隆到同一载体的同一位点吗?    ( 1 2 ) (评阅+1) (14/1924) gstracy 2016-06-18 2016-07-26 14:05:17 by pf790352975
[DNA重组] 菌液OD600为0.15,如何计算菌体数    ( 1 2 ) (14/6849) i760912798 2016-04-30 2016-07-26 14:05:08 by pf790352975
[DNA重组] [已完结]LIC克隆,T4 DNA聚合酶对平末端起作用吗? (评阅+1) (2/874) 547star 2016-07-25 2016-07-25 20:04:14 by 547star
[DNA重组] [已完结]载体构建    ( 1 2 ) (评阅+1) (17/1798) 18354255512 2016-07-21 2016-07-23 23:20:12 by 18354255512
[DNA重组] [已完结]pet28a表达引物设计问题 (评阅+1) (3/2902) 18716248350 2016-07-17 2016-07-21 22:11:00 by 独立卫生间
[DNA重组] [已完结]重组蛋白有活性吗? (评阅+1) (8/2711) yuguiyan 2016-07-15 2016-07-19 15:07:41 by 小冀-
[DNA重组] [已完结]重组质粒转化大肠杆菌后长出来的全部是载体自连的产物,怎么回事? (评阅+1) (7/3306) 小李纸11 2016-07-14 2016-07-19 12:34:45 by MMCCBB
[DNA重组] [已完结]pAX01容量问题 (评阅+1) (0/378) 不明非 2016-07-18 2016-07-18 14:34:01 by 不明非
[DNA重组] [已完结]连接产物化转提质粒浓度太低, 将质粒做化转,挑单菌落再提质粒浓度就变大了 (评阅+3) (9/2667) 潇潇溪 2015-05-06 2016-07-18 07:56:03 by 十口木水
[DNA重组] [已完结]gateway 体系BP反应问题 (评阅+1) (2/2053) 天际雾影 2016-07-02 2016-07-13 14:52:57 by taihuwuwei
[DNA重组] [已完结]蛋白酶基因片段 质粒表达 提取蛋白 (9/1105) lvyueguang 2016-07-05 2016-07-12 17:42:03 by 13365332129
[DNA重组] [已完结]用了三种菌还是表达不出蛋白    ( 1 2 3 4 5 6 ) (评阅+1) (58/3454) 上官abc 2016-05-31 2016-07-12 16:04:55 by 希希xixi
[DNA重组] [已完结]酿酒酵母导入丝状真菌基因需要去掉内含子吗? (评阅+1) (3/1112) 工大天天 2016-07-12 2016-07-12 13:30:09 by xuweitao
[DNA重组] [已完结]质粒酶切 (9/1538) shigm1121 2016-07-10 2016-07-12 09:33:54 by 18716248350
[DNA重组] [已完结]请问我的SDS-PAGE每次条带都跑得歪歪扭扭是怎么回事 (评阅+1) (6/2076) sdjnyxn123 2016-07-07 2016-07-12 09:05:50 by Bioexperixgs
[DNA重组] 菌体转到TB中长两小时就没了 (7/918) 一只毛毛虫 2016-07-08 2016-07-12 01:02:09 by lxzk
[DNA重组] [已完结]原核表达想做SDS PAGE怎么处理样品    ( 1 2 ) (评阅+1) (13/1368) yuguiyan 2016-07-10 2016-07-12 00:00:28 by yuguiyan
[DNA重组] [已完结]试剂盒纯化质粒DNA一般采用哪个公司的试剂盒比较好 (评阅+1) (2/334) tjoan33 2016-07-06 2016-07-10 22:45:55 by fengshen2005
[DNA重组] [已完结]关于pUC18连接问题 (评阅+1) (2/748) 开小猫 2016-07-07 2016-07-09 14:59:39 by 开小猫
[DNA重组] [已完结]DNA logic gates (评阅+1) (0/427) 素履之往2016 2016-07-08 2016-07-08 10:35:18 by 素履之往2016
[DNA重组] [已完结]重组质粒导入受体细胞后提质粒结果是这样的,帮忙分析一下。。。    ( 1 2 ) (评阅+1) (12/1789) 小李纸11 2016-07-02 2016-07-06 19:35:40 by 18716248350
[DNA重组] 提取质粒和基因组的区别 (2/8128) swknptb429 2016-02-26 2016-07-06 14:05:39 by tjoan33
[DNA重组] [已完结]连接T载体后双酶切两条带,单酶切也两条带    ( 1 2 ) (评阅+1) (19/1645) yuguiyan 2016-06-29 2016-07-05 22:55:03 by yuguiyan
[DNA重组] [已完结]只导入目的片段的感受态细胞能正常生长及复制基因吗? (1/1023) 小李纸11 2016-07-04 2016-07-05 09:33:08 by ls2046
[DNA重组] [已完结]大质粒提取方法 (评阅+1) (3/1432) 露露LLL 2016-03-15 2016-07-04 06:15:20 by 王平阳
[DNA重组] 染色体断裂 (0/730) 浅夏诗伤 2016-07-03 2016-07-03 16:25:19 by 浅夏诗伤
[DNA重组] [已完结]菌落PCR得到了我预期大小的条带,为啥测序是空载体?    ( 1 2 ) (12/2993) yuguiyan 2016-06-28 2016-07-02 13:30:24 by sayen
[DNA重组] [已完结]转化感受态    ( 1 2 ) (11/998) amberhwk 2016-05-31 2016-06-28 22:09:43 by ss2032
[DNA重组] 换载后,GFP就不表达了    ( 1 2 ) (10/1848) gstracy 2016-06-26 2016-06-28 07:27:19 by jackyoung
[DNA重组] [已完结]做融合蛋白的表达,这样加linker可以吗? (5/9869) gstracy 2015-06-07 2016-06-26 16:44:25 by gstracy
[DNA重组] 质粒重组挑取的单克隆出现各种不同质粒,求解?    ( 1 2 ) (评阅+1) (15/2218) 宏伟10 2016-06-20 2016-06-25 15:36:19 by 宏伟10
[DNA重组] [已完结]请问蛋白镍柱纯化之后,elution buffer洗脱下来的蛋白跟之前没什么区别是怎么回事 (9/4200) sdjnyxn123 2016-06-20 2016-06-25 01:30:31 by ttszero
[DNA重组] [已完结]不同物种体内同种酶的功能及编码该酶的基因序列相同吗? (评阅+1) (6/3549) 小李纸11 2016-06-16 2016-06-21 10:29:31 by sloop112
[DNA重组] [已完结]求载体构建 (评阅+1) (3/582) 自然茶 2016-06-20 2016-06-21 09:54:01 by 自然茶
[DNA重组] 序列拼接 (12/1163) liyuan110188 2016-06-18 2016-06-21 08:55:21 by liyuan110188
[DNA重组] 求教质粒构建后如何做定点突变    ( 1 2 ) (评阅+1) (15/4658) 549088894 2016-06-15 2016-06-20 09:00:47 by sangtian
[DNA重组] [已完结]外周血中不表达的基因是否有该基因mRNA的转录? (1/510) amokna 2015-09-20 2016-06-20 07:28:35 by 小兔血战到底
[DNA重组] 蛋白质纯化    ( 1 2 3 ) (27/1462) 凯伦爱佳佳 2016-06-02 2016-06-18 08:50:23 by baima
[DNA重组] [已完结]T-A克隆,连接目的片段后,扩出来的不是目的基因大小,怎么回事 (评阅+1) (4/1715) 18716248350 2016-06-04 2016-06-17 19:32:06 by masterboyama
[DNA重组] [已完结]将真核生物的基因在真核生物中表达还需要去掉内含子吗? (评阅+1) (2/1085) 小李纸11 2016-06-16 2016-06-17 17:17:35 by Forest666
[DNA重组] [已完结]请问七个互补碱基对可以形成双链吗,结合力会不会太弱了 (评阅+1) (2/1086) 米兔米兔 2016-06-16 2016-06-16 21:28:34 by hya1968
[DNA重组] 基因克隆 (9/727) 自然茶 2016-06-14 2016-06-16 08:44:41 by 自然茶
[DNA重组] [已完结]基因删除 (2/439) wuyuanqing 2016-06-14 2016-06-15 07:32:44 by wuyuanqing
[DNA重组] [已完结]菌P 酶切    ( 1 2 ) (评阅+1) (11/1311) 黄启秀 2016-06-08 2016-06-15 06:01:35 by 夏天的普洱茶
[DNA重组] CRISPR Case9 (0/6833) shi6625893 2016-06-13 2016-06-13 09:21:59 by shi6625893
[DNA重组] [已完结]求助含SP6、T3 和T7启动子的质粒载体有哪些? (0/5296) ldx832 2016-06-11 2016-06-11 23:30:38 by ldx832
[DNA重组] 大家谁有JM110菌株 (1/815) hnnydx 2016-06-09 2016-06-09 20:08:59 by yuguiyan
[DNA重组] 菌液PCR有条带,质粒双酶切只有一条带,是怎么回事?急!    ( 1 2 ) (11/6324) 上官abc 2016-03-04 2016-06-09 19:53:37 by yuguiyan
[DNA重组] [已完结]用菌液摇提取质粒浓度很低!    ( 1 2 3 ) (评阅+1) (23/5694) yangyang1991 2016-05-27 2016-06-08 22:20:31 by wx1308897716
[DNA重组] [已完结]multisite gateway 载体构建 (评阅+1) (1/727) dcb005 2016-06-07 2016-06-08 21:48:08 by dcb005
[DNA重组] [已完结]除了NCBI还有哪个网站可以查核酸序列? (0/1959) yuguiyan 2016-06-08 2016-06-08 17:29:25 by yuguiyan
[DNA重组] [已完结]表达载体    ( 1 2 ) (15/1159) yuan720222 2016-06-04 2016-06-07 21:49:30 by jingyu90217
[DNA重组] [已完结]有做过菌毛蛋白原核表达的虫友吗? (2/407) 安静de执着14 2016-06-07 2016-06-07 14:01:30 by 安静de执着14
[DNA重组] PCR扩增的线性片段置于载体的启动子下游的方向性 (2/737) hnnydx 2016-06-06 2016-06-06 21:17:21 by hnnydx
[DNA重组] PCR扩增的线性片段 (0/374) i760912798 2016-06-06 2016-06-06 16:29:24 by i760912798
[DNA重组] [已完结]分泌型蛋白其前体蛋白碱基突变 (0/171) 玄玄子 2016-06-06 2016-06-06 02:24:06 by 玄玄子
[DNA重组] 我怎么去鉴别这个是否是内含子的东西呢? (3/646) zlimba 2015-11-19 2016-06-04 19:38:31 by yyx0415
[DNA重组] [已完结]关于重组DNA转化宿主细胞(大肠杆菌DH5α)后阳性克隆的筛选 (评阅+1) (6/876) 小李纸11 2016-06-01 2016-06-03 16:39:54 by s4f5h7
[DNA重组] [已完结]想把氨苄青霉素抗性基因导入到金黄色葡萄球菌中,在载体构建时选择哪种质粒比较合适? (2/1012) sdtysha 2016-05-31 2016-06-02 22:32:42 by sdtysha
[DNA重组] 巢式PCR产物无法连接T载体 (0/739) siyuansing 2016-06-02 2016-06-02 18:36:27 by siyuansing
[DNA重组] Science:揭示CLIP-170微管加快肌动蛋白丝延长机制 (1/310) linglingchen 2016-05-23 2016-06-02 09:57:09 by 大susu
[DNA重组] [已完结]质粒切不开    ( 1 2 ) (16/2443) 花落已季末 2016-05-29 2016-06-01 20:38:14 by bzzwx70
[DNA重组] [已完结]急求甲基化缺陷性菌株,万分感激! (0/944) growlywolf 2016-06-01 2016-06-01 11:23:27 by growlywolf
[DNA重组] 农杆菌转化失败 (5/2600) huhu323 2016-05-30 2016-06-01 05:56:45 by yantaiquan
[DNA重组] [已完结]求pGWB5 载体序列及pDONR207载体序列 (2/2440) JMGe1990 2015-07-14 2016-05-31 20:42:47 by 阿发贝塔
[DNA重组] [已完结]侵染性克隆 (0/1541) yuan720222 2016-05-31 2016-05-31 19:55:00 by yuan720222
[DNA重组] [已完结]GK基因的异源表达与分离纯化 (评阅+1) (1/313) cuiganggang 2016-05-27 2016-05-27 22:15:27 by cuiganggang
[DNA重组] 质粒问题    ( 1 2 ) (62/2554) 三月二月88 2016-05-17 2016-05-26 10:20:47 by 三月二月88
[DNA重组] [已完结]重组质粒转化总是失败,请帮助分析下    ( 1 2 ) (11/10174) wangyibo8629 2015-07-30 2016-05-25 14:35:53 by kingjazz23
[DNA重组] [已完结][关贴]pET28a是低拷贝质粒吗    ( 1 2 3 ) (20/5430) Wawa521 2016-05-22 2016-05-25 08:09:50 by Limbo11
[DNA重组] [已完结]基因克隆问题 (2/345) 荷岚102 2016-05-23 2016-05-24 10:57:50 by 猛兽
[DNA重组] [已完结]双酶切切不出来目的条带。。。    ( 1 2 ) (13/2776) 凯伦爱佳佳 2016-03-25 2016-05-23 11:20:26 by kingjazz23
[DNA重组] [已完结]质粒双酶切后目的条带比较暗,求助改进方法 (0/2589) 410638758 2016-05-22 2016-05-22 16:34:51 by 410638758
[DNA重组] 重组构建质粒 (16/1960) 简简单单-婧 2016-05-17 2016-05-22 14:35:14 by ShenY J
[DNA重组] [已完结]NEB的限制性内切酶怎么样?提供双酶切的缓冲液么?双酶切片段都是平末端是不是不好? (9/3859) 谢伊莹 2016-05-19 2016-05-22 13:31:44 by werq63
[DNA重组] 单酶切质粒DNA片段大小比较 (1/1300) i760912798 2016-05-21 2016-05-22 00:43:56 by Eternity宇
[DNA重组] [已完结]red同源重组做敲除时 电入片段后pcr无条带?? (2/677) 淘七九懒 2015-08-31 2016-05-21 08:44:02 by 痞子无敌
[DNA重组] [已完结]带有质粒的DH5α细胞在30度培养有影响吗    ( 1 2 ) (10/2795) 找不到自己 2016-05-17 2016-05-20 18:12:43 by cencheng
[DNA重组] [已完结]高压破碎细胞BL21DE3 (5/830) 凯伦爱佳佳 2016-05-19 2016-05-20 14:11:59 by 030Selma
[DNA重组] [已完结]蛋白质纯化方法 (6/1129) 凯伦爱佳佳 2016-05-13 2016-05-20 13:00:19 by minmin1992
[DNA重组] [已完结]枯草整合质粒pDG1730的使用 (1/759) storychen 2016-05-19 2016-05-19 21:02:21 by storychen
[DNA重组] [已完结]构建重组质粒转化到大肠DH5a感受态中    ( 1 2 ) (15/2879) sh727986091 2016-05-16 2016-05-19 11:48:18 by scofield111
[DNA重组] [已完结]求一个双酶切体系 EcoR1 hind3和Bamh1 切麦谷蛋白B17亚基启动子 (6/1098) 楚地飘香 2016-05-17 2016-05-18 15:58:11 by zht930528
[DNA重组] [已完结]超声波破碎大肠杆菌试剂和条件 (2/2257) 凯伦爱佳佳 2016-05-18 2016-05-18 10:56:50 by 030Selma
[DNA重组] [已完结]danbaizhi (3/791) lhy3627981 2016-05-17 2016-05-18 08:55:01 by sloop112
[DNA重组] [已完结]请问抑菌试验中加的是菌液,还是融合蛋白,还是目的蛋白呢? (1/595) sdjnyxn123 2016-04-15 2016-05-17 16:46:33 by 781055707
[DNA重组] [已完结]有个小疑问 (2/316) 凯伦爱佳佳 2016-04-24 2016-05-17 16:44:28 by 781055707
[DNA重组] [已完结]蛋白表达效果验证,必须要用纯化过的上清或者复性后的沉淀吗? (4/808) sdjnyxn123 2016-05-09 2016-05-16 17:58:56 by sdjnyxn123
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