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[热点] 售SCI一区T0P文章，我:8.O55.1.O.54,科目全,可十急 hNpp4Y2CGvSC 2026-11-05 刚刚
[PCR相关] [已完结][关贴]在GenBank里面查找cDNA的目的是什么？ (3/1367) dswjhs 2014-05-16 2014-05-16 14:12:19 by gyesang
[PCR相关] [已完结]各位虫虫！为何我的总RNA，跑普通琼脂凝胶后，5.8s的亮度要明显高于28s和18s? (评阅+1) (1/731) liuyancheng 2014-05-15 2014-05-16 11:40:52 by 游泳的咸鱼
[PCR相关] [已完结]内参GAPDH跑PCR总是做不好，总是双峰，是怎么回事啊？    ( 1 2 ) (评阅+1) (10/7056) merfect 2014-05-13 2014-05-16 09:13:42 by jurkat.1640
[PCR相关] [已完结]全长引物一直P不出目的条带来，请大神看一下我的引物设计有没有问题？谢啦！    ( 1 2 ) (17/3706) louisjackson 2014-05-12 2014-05-15 21:27:42 by BioChen
[PCR相关] [已完结]琼脂糖凝胶电泳 求高人指点    ( 1 2 ) (13/1598) liqian901117 2014-05-14 2014-05-15 21:09:57 by 867575969
[PCR相关] [已完结]关于半定量遇到的诡异问题，求大神过来帮忙解答啊。。跪求了。。 (评阅+3) (0/508) dpzhaowei 2014-05-15 2014-05-15 17:20:59 by dpzhaowei
[PCR相关] [已完结]关于PCR连接后菌液鉴定的问题    ( 1 2 ) (18/3802) 哈皮Bingo 2014-05-13 2014-05-15 17:10:00 by 吹着泡泡的夏
[PCR相关] [已完结]关于设计克隆引物时产物片段大小有限制吗，如果我没有全长要怎么做能克隆到它的全长    ( 1 2 ) (14/3437) 小沫沫188 2014-04-08 2014-05-15 15:00:47 by 米粒儿308
[PCR相关] [已完结][关贴]为什么GenBank里查到的CDS与文献里的不一样？ (2/566) dswjhs 2014-05-14 2014-05-15 11:37:48 by dswjhs
[PCR相关] [已完结]TR-PCR时使用的λ-DNA (评阅+1) (0/1511) 小比目鲨鱼 2014-05-15 2014-05-15 10:36:32 by 小比目鲨鱼
[PCR相关] [已完结]pcr扩增出比目的片段大的片段 (2/1663) milaomai 2014-05-14 2014-05-15 03:04:41 by luwei13566
[PCR相关] [已完结]PCR小片段出不来 (评阅+1) (7/1930) 泡菜肉丝 2014-05-10 2014-05-14 22:04:38 by 泡菜肉丝
[PCR相关] [已完结]PCR扩增有的有条带有的无条带 (评阅+1) (0/739) 吹不成调 2014-05-14 2014-05-14 20:45:03 by 吹不成调
[PCR相关] [已完结]在不知道目标片段大小的情况下如何判断SSR目标片段 (评阅+3) (2/1520) 吹不成调 2014-05-07 2014-05-14 20:39:31 by 吹不成调
[PCR相关] [已完结]这是重组后的验证 (评阅+1) (7/1584) ruchang2013 2014-05-12 2014-05-14 15:27:23 by luwei13566
[PCR相关] [已完结]菌落PCR有目的条带，重组质粒提取PCR却没条带，请问是什么原因？    ( 1 2 ) (13/5006) 笑笑打dota 2014-03-20 2014-05-14 14:38:30 by beatrice1018
[PCR相关] [已完结]qPCR 内参 差异大 (4/9349) 找自己123 2014-05-13 2014-05-14 10:55:58 by Neo2000
[PCR相关] [已完结]PCR时不加模板的对照中跑出了与目的片段大小相近的条带怎么回事？    ( 1 2 ) (11/5088) 天心擎亮 2014-05-08 2014-05-14 10:55:20 by 天心擎亮
[PCR相关] [已完结]Tail-pcr相关问题 (评阅+1) (0/429) sh1018 2014-05-13 2014-05-14 08:40:53 by sh1018
[PCR相关] [已完结]pcDNA3.1上面扩增的一段序列，包括Amp区和pUC ori序列区域，但是扩增始终多一个ACC (0/1839) liuyil 2014-05-13 2014-05-13 22:52:50 by liuyil
[PCR相关] [已完结]导师希望做瘤胃微生物的mRNA差异分析，运用SSH技术可行否，有相关的最新文献吗？ (2/336) 元一妄 2014-05-13 2014-05-13 19:50:50 by Neo2000
[PCR相关] [已完结]求引物序列， (4/814) d121323 2014-05-13 2014-05-13 18:06:19 by BioChen
[PCR相关] [已完结]relative qRT-PCR有关 (评阅+1) (4/805) longxu711712 2014-04-29 2014-05-13 16:02:21 by dxd1219
[PCR相关] [已完结]荧光定量PCR内参基因 (5/1373) kekebing 2014-05-09 2014-05-12 14:22:38 by gyesang
[PCR相关] [已完结]求玉米内参基因5S rRNA的RT引物序列 (评阅+1) (1/1489) 357266258 2014-05-09 2014-05-12 13:36:59 by 357266258
[PCR相关] [已完结]PCR模板 (5/406) 潇潇杨雨125 2014-05-09 2014-05-10 23:48:50 by 潇潇杨雨125
[PCR相关] [已完结]求帮忙，如何设计tail-PCR特异引物 (3/1629) haiyanli1988 2014-05-08 2014-05-10 22:18:34 by 大-木-虫
[PCR相关] [已完结]有没有性价比高的进口PCR仪 (评阅+1) (1/513) machao8405 2014-05-10 2014-05-10 20:36:25 by BioChen
[PCR相关] [已完结]实时荧光定量pcr的一些问题 (评阅+1) (0/1122) lanlvmaomao 2014-05-10 2014-05-10 10:34:31 by lanlvmaomao
[PCR相关] [已完结]目的条带P不出来！ (5/1480) 18252091017 2014-05-08 2014-05-09 18:17:06 by 快乐农夫
[PCR相关] [已完结]求经验值！ (5/612) jkamm 2014-05-08 2014-05-09 15:25:46 by youlinglyw
[PCR相关] [已完结]miRNA引物合成 (1/1145) feilinshiji 2014-05-09 2014-05-09 14:35:37 by biostar2009
[PCR相关] [已完结]求助检测基因沉默的qPCR引物设计问题 (7/3037) greenygreen 2014-05-07 2014-05-09 09:06:36 by greenygreen
[PCR相关] [已完结]我的RT-CR有什么问题 (3/1227) fistwl 2014-05-08 2014-05-09 09:04:24 by fistwl
[PCR相关] [已完结]跑胶条带，帮忙分析下点样孔很亮的原因 (1/4429) dispy 2014-05-08 2014-05-09 03:47:34 by meng_xu
[PCR相关] [已完结]荧光定量PCR 校准组的设置？求助 (1/1143) maxrog 2014-05-08 2014-05-08 19:57:31 by junnavme2014
[PCR相关] [已完结]8kbp怎么做PCR?    ( 1 2 ) (10/1523) 致远012 2014-04-02 2014-05-08 13:25:37 by willzhuu
[PCR相关] [已完结]求祝各位大神哈，DNA 片段放置时间过长是否会影响连接效率？？？ (评阅+1) (4/1372) ottoKING 2014-04-27 2014-05-08 10:10:10 by lishuiqing
[PCR相关] [已完结]RNA反转录后引物扩增结果分析 (2/883) Jianxiao_H 2014-05-07 2014-05-08 08:05:45 by Jianxiao_H
[PCR相关] [已完结]RACE拼接后扩增全长的测序结果不一样 (5/2119) okletsgo 2014-05-07 2014-05-07 22:07:55 by oylb
[PCR相关] [已完结]如何设计点突变引物？    ( 1 2 ) (14/14650) huangwy927 2014-02-08 2014-05-07 17:23:43 by wlwhappy
[PCR相关] [已完结][关贴]求助分析酶切图，急急急！！！！ (评阅+1) (2/516) sddabc 2014-05-07 2014-05-07 16:17:03 by sddabc
[PCR相关] [已完结]是否为引物二聚体 (评阅+1) (7/1727) lh318 2014-04-29 2014-05-07 16:10:38 by lh318
[PCR相关] [已完结]PCR相关问题。。求指导。。谢谢 (3/846) liulugang 2014-05-06 2014-05-07 03:37:34 by meng_xu
[PCR相关] [已完结]求助：Pml I 和Sfi I 的酶切位点和保护碱基 (0/5359) katrina_yao 2014-05-06 2014-05-06 22:19:21 by katrina_yao
[PCR相关] [已完结]Promega的M-MLV酶做反转录体系中Oligo（dT）的浓度是多少？ (评阅+1) (1/2280) 清辉水木 2014-03-15 2014-05-06 16:44:41 by biology-boy
[PCR相关] [已完结]请问一下，连接转化完的菌液送去测序，快递了4天了还没到测序公司，会不会坏掉啊    ( 1 2 ) (13/3273) jmh5d 2014-05-03 2014-05-06 10:44:08 by BioChen
[PCR相关] 为什么我用gDNA连一个3kb的片段都扩不出来？    ( 1 2 ) (评阅+1) (12/2053) Carnity 2014-04-29 2014-05-06 10:04:44 by 朱多龙
[PCR相关] [已完结]PCR产物回收胶的保存    ( 1 2 ) (15/2833) xielifenxie 2014-04-28 2014-05-05 19:01:08 by yanzhuo
[PCR相关] [已完结]gene库里面没有包含的种属---如何设计引物 (2/1132) kindbigbear0 2014-04-01 2014-05-05 13:08:53 by kindbigbear0
[PCR相关] [已完结]最近做pcr,内参出来了，目的片段却一直出不来，只有引物二聚体    ( 1 2 ) (14/3014) dingcong1216 2014-04-30 2014-05-05 09:47:34 by dingcong1216
[PCR相关] ChIP-QPCR (评阅+1) (8/4745) 宙钰乾坤 2014-04-29 2014-05-05 09:25:05 by yipan
[PCR相关] [已完结]16 S rDNA能否做内参 (评阅+1) (0/1454) qinghuaihulu 2014-05-04 2014-05-04 16:55:49 by qinghuaihulu
[PCR相关] [已完结]pcr出现类似弥散问题怎么回事，帮我分析分析原因，谢谢 (1/678) 意萧02 2014-05-04 2014-05-04 16:29:32 by 1057910381
[PCR相关] [已完结]在实时荧光PCR中参照基因是用25s rRNA还β-actin比较好？ (5/777) 小比目鲨鱼 2014-04-30 2014-05-04 15:39:47 by Neo2000
[PCR相关] [已完结][关贴]我犯了一个挺有意思的错误，大家进来讨论一下吧 (7/1389) wenzhipeng 2014-04-30 2014-05-04 15:39:38 by Asura-
[PCR相关] [已完结]qRT-PCR, 组织特异性表达 (评阅+1) (0/1304) 麦水螅 2014-05-04 2014-05-04 09:13:34 by 麦水螅
[PCR相关] [已完结]什么是PCR冷启动？ (2/2644) 半调子梅花 2014-05-03 2014-05-03 15:29:41 by 半调子梅花
[PCR相关] 请问巢式pcr的灵敏度大概有多少？ (2/1436) 昨日牛郎 2014-05-02 2014-05-03 12:20:43 by meng_xu
[PCR相关] [已完结]SSR转化SCAR 引物设计 (0/536) Botak 2014-05-02 2014-05-02 12:31:15 by Botak
[PCR相关] [已完结]ISSR-PCR标记电泳图 (6/1904) penny923 2014-04-01 2014-05-02 10:55:23 by timesty
[PCR相关] [已完结]琼脂糖凝胶电泳严重拖尾 (4/2186) sinky熹 2014-05-01 2014-05-02 09:56:54 by yuren2009
[PCR相关] [已完结]阳性克隆的鉴定 (5/1939) 崔永霞 2014-04-30 2014-05-01 12:29:03 by zep616745243
[PCR相关] [已完结]大家有没有遇到这种情况？ (4/1049) 木vs木 2014-04-30 2014-05-01 10:18:23 by 西门吹雪170
[PCR相关] 有没有人遇到过跑胶时PCR产物会减少的现象，请大神们指教 (7/1724) houyuru1990 2014-04-30 2014-04-30 20:26:28 by pennchem
[PCR相关] [已完结]保真性太差，200多的片段有6-7个bp的错配    ( 1 2 3 4 ) (33/2150) leiqin 2014-04-27 2014-04-30 12:27:34 by shuifen1108
[PCR相关] [已完结]关于筛选htSNP。 (0/521) David勇 2014-04-30 2014-04-30 12:01:20 by David勇
[PCR相关] [已完结]下游引物设计求助 (1/1606) cassie小萍 2014-04-29 2014-04-29 18:11:54 by 西门吹雪170
[PCR相关] [已完结]PCR电泳图请帮忙分析    ( 1 2 ) (评阅+1) (10/1896) lumy790101 2014-04-23 2014-04-29 14:51:40 by lumy790101
[PCR相关] [关贴]求帮助 (2/385) guminghong 2014-04-29 2014-04-29 14:14:49 by Rubisco580
[PCR相关] [已完结]甲基化敏感性内切酶 (评阅+1) (MolEPI+1)(2/7729) r-rainbow 2014-04-29 2014-04-29 12:24:50 by biostar2009
[PCR相关] [已完结]PCR第一次扩出来第二次扩不出来 (7/2119) er66668888 2014-03-30 2014-04-29 11:27:06 by zhaoxiaopang
[PCR相关] [已完结]PCR，电泳 (评阅+1) (5/778) summit061800 2014-04-24 2014-04-29 10:09:48 by zhaoxiaopang
[PCR相关] 请问大家PCR扩增的最长产物是多少bp    ( 1 2 ) (10/6131) 283503264 2014-04-09 2014-04-29 09:41:20 by 283503264
[PCR相关] [已完结]有关是不是引物二聚体的分析    ( 1 2 ) (10/2528) 三蛰 2014-03-23 2014-04-28 23:58:33 by zep616745243
[PCR相关] [已完结]荧光定量 (评阅+1) (0/227) tuhua 2014-04-28 2014-04-28 21:53:34 by tuhua
[PCR相关] [已完结]实时定量PCR荧光值出现过早，但荧光值先稍微升高后又下降？ (2/1169) 大甫哥 2014-04-27 2014-04-28 19:35:16 by 大甫哥
[PCR相关] [已完结]最近测了1700 bp的一段序列，用的是PCR产物，结果是前后40 bp左右没测出来 (4/1029) liuyil 2014-04-27 2014-04-28 16:59:42 by 青苹果QT
[PCR相关] [已完结]PCR产物测序 (2/515) 呀薯条 2014-04-28 2014-04-28 13:34:38 by zep616745243
[PCR相关] [已完结]荧光定量PCR (8/1017) xiayu608 2014-04-24 2014-04-28 10:09:15 by 邹忌
[PCR相关] [已完结]用cdna跑不出目的条带，反而用dna跑出了目的条带，实在搞不懂了 (3/2271) 想学好的学渣 2014-04-27 2014-04-28 09:57:25 by 想学好的学渣
[PCR相关] [已完结]我的条带小于目的条带。。。求助。。。。    ( 1 2 ) (15/2645) hhl1230 2014-04-23 2014-04-27 19:57:29 by Hliotrope
[PCR相关] [已完结]全长cDNA PCR问题 (评阅+1) (4/1097) ashleyoo00 2014-04-26 2014-04-27 10:54:44 by ashleyoo00
[PCR相关] [已完结]查找启动子 (评阅+1) (2/2298) 花花NO.1 2014-04-24 2014-04-26 22:29:48 by 凌波丽
[PCR相关] [已完结]英雄，HELP！ 这个基因死活扩不出来啊 (1/775) KEVIN_LOVE 2014-04-25 2014-04-26 16:39:28 by br24
[PCR相关] [已完结]RT-PCR (3/958) 伊梦青lhq 2014-04-25 2014-04-26 12:35:28 by 伊梦青lhq
[PCR相关] 噬菌体展示 pcr 没带 什么原因 (1/1440) linyingjia 2014-04-25 2014-04-26 09:54:32 by 凌波丽
[PCR相关] [已完结]Nde I 和 Not I 可以一起用吗？ (3/1372) 小迷糊912 2014-04-23 2014-04-25 18:58:53 by 小迷糊912
[PCR相关] [已完结]DGGE实验动物样本本身个体差异大怎么办 (0/821) 小虫5903 2014-04-25 2014-04-25 10:53:13 by 小虫5903
[PCR相关] [已完结]转化后平板不长菌    ( 1 2 ) (19/3950) 最末那抹夕阳 2014-04-21 2014-04-25 01:18:11 by jukongka
[PCR相关] [已完结]荧光定量检测不到信号 (2/1406) 熙玥 2014-04-24 2014-04-24 23:15:49 by 熙玥
[PCR相关] PCR引物是选择特异性引物还是oligo (0/2358) suzhou1989 2014-04-24 2014-04-24 16:25:36 by suzhou1989
[PCR相关] [已完结]SSR引物筛选 (0/1340) penny923 2014-04-24 2014-04-24 13:45:10 by penny923
[PCR相关] [已完结]荧光定量PCR (5/759) xiayu608 2014-04-23 2014-04-24 10:59:22 by jurkat.1640
[PCR相关] [已完结]做real time PCR时为什么不能先把DNA都加进去再分装 (5/856) crazymouse66 2014-04-23 2014-04-24 10:13:19 by cituyuqi
[PCR相关] [已完结]菌液PCR验证 (评阅+1) (7/1036) wanghf_6134 2014-03-30 2014-04-24 08:35:23 by XOooZzz
[PCR相关] [已完结]大家认为判断引物设计合理的主要依据有哪些 (评阅+1) (7/4304) 简单的执着 2014-04-21 2014-04-23 23:41:24 by gyesang
[PCR相关] [已完结]做真菌（最好是曲霉类）siRNA或miRNA PCR检测时的内参基因大家都用的什么？ (评阅+1) (0/1713) bbyu007 2014-04-23 2014-04-23 17:33:08 by bbyu007
[PCR相关] [已完结]大侠帮我看看，这个嵌合体检测结果怎么看啊，没做过啊 (0/3710) cya_yanan 2014-04-23 2014-04-23 15:33:03 by cya_yanan
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