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[热点] 天津大学招2026.09的博士生，欢迎大家推荐交流（博导是本人） a793625982 2026-02-07 刚刚
[PCR相关] [已完结]RNA反转录后引物扩增结果分析 (2/791) Jianxiao_H 2014-05-07 2014-05-08 08:05:45 by Jianxiao_H
[PCR相关] [已完结]RACE拼接后扩增全长的测序结果不一样 (5/1759) okletsgo 2014-05-07 2014-05-07 22:07:55 by oylb
[PCR相关] [已完结]如何设计点突变引物？    ( 1 2 ) (14/13990) huangwy927 2014-02-08 2014-05-07 17:23:43 by wlwhappy
[PCR相关] [已完结][关贴]求助分析酶切图，急急急！！！！ (评阅+1) (2/300) sddabc 2014-05-07 2014-05-07 16:17:03 by sddabc
[PCR相关] [已完结]是否为引物二聚体 (评阅+1) (7/1466) lh318 2014-04-29 2014-05-07 16:10:38 by lh318
[PCR相关] [已完结]PCR相关问题。。求指导。。谢谢 (3/671) liulugang 2014-05-06 2014-05-07 03:37:34 by meng_xu
[PCR相关] [已完结]求助：Pml I 和Sfi I 的酶切位点和保护碱基 (0/5197) katrina_yao 2014-05-06 2014-05-06 22:19:21 by katrina_yao
[PCR相关] [已完结]Promega的M-MLV酶做反转录体系中Oligo（dT）的浓度是多少？ (评阅+1) (1/2210) 清辉水木 2014-03-15 2014-05-06 16:44:41 by biology-boy
[PCR相关] [已完结]请问一下，连接转化完的菌液送去测序，快递了4天了还没到测序公司，会不会坏掉啊    ( 1 2 ) (13/2798) jmh5d 2014-05-03 2014-05-06 10:44:08 by BioChen
[PCR相关] 为什么我用gDNA连一个3kb的片段都扩不出来？    ( 1 2 ) (评阅+1) (12/1808) Carnity 2014-04-29 2014-05-06 10:04:44 by 朱多龙
[PCR相关] [已完结]PCR产物回收胶的保存    ( 1 2 ) (15/2361) xielifenxie 2014-04-28 2014-05-05 19:01:08 by yanzhuo
[PCR相关] [已完结]gene库里面没有包含的种属---如何设计引物 (2/1053) kindbigbear0 2014-04-01 2014-05-05 13:08:53 by kindbigbear0
[PCR相关] [已完结]最近做pcr,内参出来了，目的片段却一直出不来，只有引物二聚体    ( 1 2 ) (14/2465) dingcong1216 2014-04-30 2014-05-05 09:47:34 by dingcong1216
[PCR相关] ChIP-QPCR (评阅+1) (8/4507) 宙钰乾坤 2014-04-29 2014-05-05 09:25:05 by yipan
[PCR相关] [已完结]16 S rDNA能否做内参 (评阅+1) (0/1406) qinghuaihulu 2014-05-04 2014-05-04 16:55:49 by qinghuaihulu
[PCR相关] [已完结]pcr出现类似弥散问题怎么回事，帮我分析分析原因，谢谢 (1/561) 意萧02 2014-05-04 2014-05-04 16:29:32 by 1057910381
[PCR相关] [已完结]在实时荧光PCR中参照基因是用25s rRNA还β-actin比较好？ (5/619) 小比目鲨鱼 2014-04-30 2014-05-04 15:39:47 by Neo2000
[PCR相关] [已完结][关贴]我犯了一个挺有意思的错误，大家进来讨论一下吧 (7/1060) wenzhipeng 2014-04-30 2014-05-04 15:39:38 by Asura-
[PCR相关] [已完结]qRT-PCR, 组织特异性表达 (评阅+1) (0/1254) 麦水螅 2014-05-04 2014-05-04 09:13:34 by 麦水螅
[PCR相关] [已完结]什么是PCR冷启动？ (2/2523) 半调子梅花 2014-05-03 2014-05-03 15:29:41 by 半调子梅花
[PCR相关] 请问巢式pcr的灵敏度大概有多少？ (2/1320) 昨日牛郎 2014-05-02 2014-05-03 12:20:43 by meng_xu
[PCR相关] [已完结]SSR转化SCAR 引物设计 (0/495) Botak 2014-05-02 2014-05-02 12:31:15 by Botak
[PCR相关] [已完结]ISSR-PCR标记电泳图 (6/1597) penny923 2014-04-01 2014-05-02 10:55:23 by timesty
[PCR相关] [已完结]琼脂糖凝胶电泳严重拖尾 (4/1951) sinky熹 2014-05-01 2014-05-02 09:56:54 by yuren2009
[PCR相关] [已完结]阳性克隆的鉴定 (5/1765) 崔永霞 2014-04-30 2014-05-01 12:29:03 by zep616745243
[PCR相关] [已完结]大家有没有遇到这种情况？ (4/851) 木vs木 2014-04-30 2014-05-01 10:18:23 by 西门吹雪170
[PCR相关] 有没有人遇到过跑胶时PCR产物会减少的现象，请大神们指教 (7/1287) houyuru1990 2014-04-30 2014-04-30 20:26:28 by pennchem
[PCR相关] [已完结]保真性太差，200多的片段有6-7个bp的错配    ( 1 2 3 4 ) (33/1571) leiqin 2014-04-27 2014-04-30 12:27:34 by shuifen1108
[PCR相关] [已完结]关于筛选htSNP。 (0/473) David勇 2014-04-30 2014-04-30 12:01:20 by David勇
[PCR相关] [已完结]下游引物设计求助 (1/1535) cassie小萍 2014-04-29 2014-04-29 18:11:54 by 西门吹雪170
[PCR相关] [已完结]PCR电泳图请帮忙分析    ( 1 2 ) (评阅+1) (10/1583) lumy790101 2014-04-23 2014-04-29 14:51:40 by lumy790101
[PCR相关] [关贴]求帮助 (2/298) guminghong 2014-04-29 2014-04-29 14:14:49 by Rubisco580
[PCR相关] [已完结]甲基化敏感性内切酶 (评阅+1) (MolEPI+1)(2/7363) r-rainbow 2014-04-29 2014-04-29 12:24:50 by biostar2009
[PCR相关] [已完结]PCR第一次扩出来第二次扩不出来 (7/1679) er66668888 2014-03-30 2014-04-29 11:27:06 by zhaoxiaopang
[PCR相关] [已完结]PCR，电泳 (评阅+1) (5/625) summit061800 2014-04-24 2014-04-29 10:09:48 by zhaoxiaopang
[PCR相关] 请问大家PCR扩增的最长产物是多少bp    ( 1 2 ) (10/5598) 283503264 2014-04-09 2014-04-29 09:41:20 by 283503264
[PCR相关] [已完结]有关是不是引物二聚体的分析    ( 1 2 ) (10/1972) 三蛰 2014-03-23 2014-04-28 23:58:33 by zep616745243
[PCR相关] [已完结]荧光定量 (评阅+1) (0/200) tuhua 2014-04-28 2014-04-28 21:53:34 by tuhua
[PCR相关] [已完结]实时定量PCR荧光值出现过早，但荧光值先稍微升高后又下降？ (2/1085) 大甫哥 2014-04-27 2014-04-28 19:35:16 by 大甫哥
[PCR相关] [已完结]最近测了1700 bp的一段序列，用的是PCR产物，结果是前后40 bp左右没测出来 (4/843) liuyil 2014-04-27 2014-04-28 16:59:42 by 青苹果QT
[PCR相关] [已完结]PCR产物测序 (2/402) 呀薯条 2014-04-28 2014-04-28 13:34:38 by zep616745243
[PCR相关] [已完结]荧光定量PCR (8/800) xiayu608 2014-04-24 2014-04-28 10:09:15 by 邹忌
[PCR相关] [已完结]用cdna跑不出目的条带，反而用dna跑出了目的条带，实在搞不懂了 (3/2069) 想学好的学渣 2014-04-27 2014-04-28 09:57:25 by 想学好的学渣
[PCR相关] [已完结]我的条带小于目的条带。。。求助。。。。    ( 1 2 ) (15/2023) hhl1230 2014-04-23 2014-04-27 19:57:29 by Hliotrope
[PCR相关] [已完结]全长cDNA PCR问题 (评阅+1) (4/755) ashleyoo00 2014-04-26 2014-04-27 10:54:44 by ashleyoo00
[PCR相关] [已完结]查找启动子 (评阅+1) (2/2116) 花花NO.1 2014-04-24 2014-04-26 22:29:48 by 凌波丽
[PCR相关] [已完结]英雄，HELP！ 这个基因死活扩不出来啊 (1/645) KEVIN_LOVE 2014-04-25 2014-04-26 16:39:28 by br24
[PCR相关] [已完结]RT-PCR (3/786) 伊梦青lhq 2014-04-25 2014-04-26 12:35:28 by 伊梦青lhq
[PCR相关] 噬菌体展示 pcr 没带 什么原因 (1/1309) linyingjia 2014-04-25 2014-04-26 09:54:32 by 凌波丽
[PCR相关] [已完结]Nde I 和 Not I 可以一起用吗？ (3/1198) 小迷糊912 2014-04-23 2014-04-25 18:58:53 by 小迷糊912
[PCR相关] [已完结]DGGE实验动物样本本身个体差异大怎么办 (0/783) 小虫5903 2014-04-25 2014-04-25 10:53:13 by 小虫5903
[PCR相关] [已完结]转化后平板不长菌    ( 1 2 ) (19/3385) 最末那抹夕阳 2014-04-21 2014-04-25 01:18:11 by jukongka
[PCR相关] [已完结]荧光定量检测不到信号 (2/1189) 熙玥 2014-04-24 2014-04-24 23:15:49 by 熙玥
[PCR相关] PCR引物是选择特异性引物还是oligo (0/2308) suzhou1989 2014-04-24 2014-04-24 16:25:36 by suzhou1989
[PCR相关] [已完结]SSR引物筛选 (0/1303) penny923 2014-04-24 2014-04-24 13:45:10 by penny923
[PCR相关] [已完结]荧光定量PCR (5/651) xiayu608 2014-04-23 2014-04-24 10:59:22 by jurkat.1640
[PCR相关] [已完结]做real time PCR时为什么不能先把DNA都加进去再分装 (5/662) crazymouse66 2014-04-23 2014-04-24 10:13:19 by cituyuqi
[PCR相关] [已完结]菌液PCR验证 (评阅+1) (7/819) wanghf_6134 2014-03-30 2014-04-24 08:35:23 by XOooZzz
[PCR相关] [已完结]大家认为判断引物设计合理的主要依据有哪些 (评阅+1) (7/4023) 简单的执着 2014-04-21 2014-04-23 23:41:24 by gyesang
[PCR相关] [已完结]做真菌（最好是曲霉类）siRNA或miRNA PCR检测时的内参基因大家都用的什么？ (评阅+1) (0/1589) bbyu007 2014-04-23 2014-04-23 17:33:08 by bbyu007
[PCR相关] [已完结]大侠帮我看看，这个嵌合体检测结果怎么看啊，没做过啊 (0/3657) cya_yanan 2014-04-23 2014-04-23 15:33:03 by cya_yanan
[PCR相关] [已完结]做转化时，感受态用多大的量比较好呢    ( 1 2 ) (15/2565) 柠檬可乐星 2014-04-17 2014-04-23 14:31:12 by 柠檬可乐星
[PCR相关] [已完结]如何确定自己的目的片段 PCR 痛苦中    ( 1 2 ) (17/2655) wanglixia815 2014-04-20 2014-04-23 13:34:37 by 凌枫miracle
[PCR相关] [已完结]RT- PCR 跑出来的带有几条，求助 (评阅+1) (6/897) 唐萌2 2014-04-15 2014-04-23 00:51:15 by xygcdmr
[PCR相关] [已完结]PCR一直p不出来，求大神指点（for DGGE） (2/549) xhy315 2014-04-10 2014-04-22 19:50:04 by yinlizhangli
[PCR相关] [已完结]求大神分析一下这次的PCR结果 (评阅+1) (1/276) 冰糖-葫芦娃 2014-04-16 2014-04-22 15:21:20 by 邹忌
[PCR相关] [已完结]分析电泳图，求神人过来啊~~救命啊~~！！！指点啊    ( 1 2 ) (10/1144) 小小鱼泪了 2014-04-21 2014-04-22 13:29:49 by jiaolong11a
[PCR相关] [已完结]用链霉菌DNA做荧光定量PCR，相对定量？ (评阅+1) (0/399) benita-chen 2014-04-22 2014-04-22 10:42:31 by benita-chen
[PCR相关] [已完结]相关引物 (0/192) wm阳光 2014-04-21 2014-04-21 20:38:11 by wm阳光
[PCR相关] [已完结]已经快PCR折磨疯了。。救命呀    ( 1 2 3 4 5 ) (评阅+1) (41/2257) wanglixia815 2014-04-09 2014-04-21 17:30:07 by lsg-ll
[PCR相关] [已完结]麻烦各位看一下我溶解曲线的结果 (1/1321) longyuming 2014-04-20 2014-04-21 15:50:14 by zhangyunjing
[PCR相关] [已完结]扩增质粒的技术方法 (1/790) 发酵与酶工程 2014-04-20 2014-04-20 23:49:31 by huangrui1988
[PCR相关] 连接、转化以及酶切问题 (评阅+1) (0/534) 蜜桃椰果 2014-04-20 2014-04-20 18:35:39 by 蜜桃椰果
[PCR相关] [已完结]WRKY转录因子克隆 (4/558) 许愿猫 2014-04-19 2014-04-20 14:57:41 by 许愿猫
[PCR相关] [已完结]DNA回收产物浓度一般是多少？    ( 1 2 ) (11/10432) 呀薯条 2014-04-10 2014-04-19 16:58:56 by xiaopangge
[PCR相关] [已完结][高分求助]~NDA测序 (2/527) 我们一起 2014-04-17 2014-04-19 13:42:30 by 我们一起
[PCR相关] [已完结]PMD 19-t和PMD 18-t那个好使 (3/3264) 柠檬可乐星 2014-04-18 2014-04-19 10:17:22 by KEVIN_LOVE
[PCR相关] [已完结]细菌real time RT-PCR (3/750) qinghuaihulu 2014-04-16 2014-04-18 21:58:35 by qinghuaihulu
[PCR相关] [已完结]求教高手2×KOD PCR Master Mix的组分，急！！！ (0/355) lixihailove 2014-04-18 2014-04-18 19:57:08 by lixihailove
[PCR相关] 关于microRNA q RT-PCR绝对定量 (4/2227) zhpu2008 2014-04-15 2014-04-18 16:54:07 by OmicsLink
[PCR相关] [已完结]融合PCR (0/395) panda传奇 2014-04-18 2014-04-18 15:43:26 by panda传奇
[PCR相关] [已完结]基因克隆 (评阅+1) (3/364) 许愿猫 2014-04-17 2014-04-18 12:45:45 by zhaozhibozhi
[PCR相关] [已完结]扩增出了比目的条带更长的杂带，是什么原因？ (2/819) 柠檬可乐星 2014-04-17 2014-04-18 09:57:48 by 柠檬可乐星
[PCR相关] [已完结]斑马鱼的Race（快速cDNA莫端克隆）实验 (6/863) aalearner 2014-04-16 2014-04-17 13:36:01 by aalearner
[PCR相关] [已完结]Real-time PCR 的数据如果跟其他数据不吻合怎么办？ (评阅+1) (4/808) bradyluo 2014-04-16 2014-04-17 13:26:56 by sanyou2007
[PCR相关] [已完结]RACE和Genome Walking 的区别？ (1/1516) 小海豚1987 2014-04-17 2014-04-17 12:24:54 by Neo2000
[PCR相关] [已完结]大侠请求吧帮助，关于筛选siRNA及PCR (0/285) 小瞎子 2014-04-17 2014-04-17 12:06:20 by 小瞎子
[PCR相关] 验钞的紫外手电筒可以照EB胶吗？能看到吗？ (15/1953) fanwq258 2014-02-28 2014-04-17 09:36:48 by fanwq258
[PCR相关] [已完结]请教HPV 16 mRNA序列问题 (0/545) 木木803 2014-04-17 2014-04-17 09:24:41 by 木木803
[PCR相关] [已完结]求助怎么解释动物体内蛋白跟基因表达不一致 (0/2075) xu523130761 2014-04-17 2014-04-17 09:19:05 by xu523130761
[PCR相关] [已完结]为什么引物敏感性很低？ (评阅+1) (1/662) 永远一片天 2014-04-16 2014-04-17 07:44:12 by Neo2000
[PCR相关] [已完结]咨询一下AFLP分型的费用，现在还有做这样的公司吗 (7/678) 前路知己 2014-04-14 2014-04-16 19:16:07 by 前路知己
[PCR相关] [已完结]LAMP时用哪家公司哪个货号的Bst酶和甜菜碱 (5/1930) 谢伊莹 2014-04-10 2014-04-16 11:05:51 by tiaotiaohu0
[PCR相关] [已完结]PCR之后胶回收未见条带 (2/458) zhian.5177 2014-04-16 2014-04-16 10:58:52 by 867575969
[PCR相关] [已完结]DNAstar win7系统下安装 (0/705) 学员YjPBB2 2014-04-16 2014-04-16 10:17:00 by zw227
[PCR相关] [已完结]定量pcr做黄了，求失败原因 (评阅+1) (0/468) xcrec 2014-04-15 2014-04-15 22:15:59 by xcrec
[PCR相关] [已完结]为什么用pcr产物作为模版以后p不出东西？ (2/1138) chong1217 2014-04-14 2014-04-15 19:47:39 by chong1217
[PCR相关] [已完结]菌落PCR    ( 1 2 ) (11/1381) 呀薯条 2014-04-14 2014-04-15 18:54:57 by 不爱做实验
[PCR相关] [已完结]有关TA克隆连接问题 (评阅+1) (2/1474) 瓶中飞羽 2014-04-15 2014-04-15 17:59:02 by for5
[PCR相关] [已完结]M13通用引物 (5/11311) 呀薯条 2014-04-15 2014-04-15 12:22:23 by d00614028
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