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[热点] 面上提前没消息，有中的吗 archvillain 2026-08-08 刚刚
[PCR相关] [已完结]PCR扩增有的有条带有的无条带 (评阅+1) (0/713) 吹不成调 2014-05-14 2014-05-14 20:45:03 by 吹不成调
[PCR相关] [已完结]在不知道目标片段大小的情况下如何判断SSR目标片段 (评阅+3) (2/1437) 吹不成调 2014-05-07 2014-05-14 20:39:31 by 吹不成调
[PCR相关] [已完结]这是重组后的验证 (评阅+1) (7/1442) ruchang2013 2014-05-12 2014-05-14 15:27:23 by luwei13566
[PCR相关] [已完结]菌落PCR有目的条带，重组质粒提取PCR却没条带，请问是什么原因？    ( 1 2 ) (13/4823) 笑笑打dota 2014-03-20 2014-05-14 14:38:30 by beatrice1018
[PCR相关] [已完结]qPCR 内参 差异大 (4/9251) 找自己123 2014-05-13 2014-05-14 10:55:58 by Neo2000
[PCR相关] [已完结]PCR时不加模板的对照中跑出了与目的片段大小相近的条带怎么回事？    ( 1 2 ) (11/4943) 天心擎亮 2014-05-08 2014-05-14 10:55:20 by 天心擎亮
[PCR相关] [已完结]Tail-pcr相关问题 (评阅+1) (0/411) sh1018 2014-05-13 2014-05-14 08:40:53 by sh1018
[PCR相关] [已完结]pcDNA3.1上面扩增的一段序列，包括Amp区和pUC ori序列区域，但是扩增始终多一个ACC (0/1819) liuyil 2014-05-13 2014-05-13 22:52:50 by liuyil
[PCR相关] [已完结]导师希望做瘤胃微生物的mRNA差异分析，运用SSH技术可行否，有相关的最新文献吗？ (2/307) 元一妄 2014-05-13 2014-05-13 19:50:50 by Neo2000
[PCR相关] [已完结]求引物序列， (4/724) d121323 2014-05-13 2014-05-13 18:06:19 by BioChen
[PCR相关] [已完结]relative qRT-PCR有关 (评阅+1) (4/752) longxu711712 2014-04-29 2014-05-13 16:02:21 by dxd1219
[PCR相关] [已完结]荧光定量PCR内参基因 (5/1305) kekebing 2014-05-09 2014-05-12 14:22:38 by gyesang
[PCR相关] [已完结]求玉米内参基因5S rRNA的RT引物序列 (评阅+1) (1/1460) 357266258 2014-05-09 2014-05-12 13:36:59 by 357266258
[PCR相关] [已完结]PCR模板 (5/378) 潇潇杨雨125 2014-05-09 2014-05-10 23:48:50 by 潇潇杨雨125
[PCR相关] [已完结]求帮忙，如何设计tail-PCR特异引物 (3/1576) haiyanli1988 2014-05-08 2014-05-10 22:18:34 by 大-木-虫
[PCR相关] [已完结]有没有性价比高的进口PCR仪 (评阅+1) (1/492) machao8405 2014-05-10 2014-05-10 20:36:25 by BioChen
[PCR相关] [已完结]实时荧光定量pcr的一些问题 (评阅+1) (0/1082) lanlvmaomao 2014-05-10 2014-05-10 10:34:31 by lanlvmaomao
[PCR相关] [已完结]目的条带P不出来！ (5/1392) 18252091017 2014-05-08 2014-05-09 18:17:06 by 快乐农夫
[PCR相关] [已完结]求经验值！ (5/556) jkamm 2014-05-08 2014-05-09 15:25:46 by youlinglyw
[PCR相关] [已完结]miRNA引物合成 (1/1097) feilinshiji 2014-05-09 2014-05-09 14:35:37 by biostar2009
[PCR相关] [已完结]求助检测基因沉默的qPCR引物设计问题 (7/2882) greenygreen 2014-05-07 2014-05-09 09:06:36 by greenygreen
[PCR相关] [已完结]我的RT-CR有什么问题 (3/1124) fistwl 2014-05-08 2014-05-09 09:04:24 by fistwl
[PCR相关] [已完结]跑胶条带，帮忙分析下点样孔很亮的原因 (1/4387) dispy 2014-05-08 2014-05-09 03:47:34 by meng_xu
[PCR相关] [已完结]荧光定量PCR 校准组的设置？求助 (1/1112) maxrog 2014-05-08 2014-05-08 19:57:31 by junnavme2014
[PCR相关] [已完结]8kbp怎么做PCR?    ( 1 2 ) (10/1396) 致远012 2014-04-02 2014-05-08 13:25:37 by willzhuu
[PCR相关] [已完结]求祝各位大神哈，DNA 片段放置时间过长是否会影响连接效率？？？ (评阅+1) (4/1318) ottoKING 2014-04-27 2014-05-08 10:10:10 by lishuiqing
[PCR相关] [已完结]RNA反转录后引物扩增结果分析 (2/848) Jianxiao_H 2014-05-07 2014-05-08 08:05:45 by Jianxiao_H
[PCR相关] [已完结]RACE拼接后扩增全长的测序结果不一样 (5/2008) okletsgo 2014-05-07 2014-05-07 22:07:55 by oylb
[PCR相关] [已完结]如何设计点突变引物？    ( 1 2 ) (14/14335) huangwy927 2014-02-08 2014-05-07 17:23:43 by wlwhappy
[PCR相关] [已完结][关贴]求助分析酶切图，急急急！！！！ (评阅+1) (2/402) sddabc 2014-05-07 2014-05-07 16:17:03 by sddabc
[PCR相关] [已完结]是否为引物二聚体 (评阅+1) (7/1625) lh318 2014-04-29 2014-05-07 16:10:38 by lh318
[PCR相关] [已完结]PCR相关问题。。求指导。。谢谢 (3/751) liulugang 2014-05-06 2014-05-07 03:37:34 by meng_xu
[PCR相关] [已完结]求助：Pml I 和Sfi I 的酶切位点和保护碱基 (0/5298) katrina_yao 2014-05-06 2014-05-06 22:19:21 by katrina_yao
[PCR相关] [已完结]Promega的M-MLV酶做反转录体系中Oligo（dT）的浓度是多少？ (评阅+1) (1/2259) 清辉水木 2014-03-15 2014-05-06 16:44:41 by biology-boy
[PCR相关] [已完结]请问一下，连接转化完的菌液送去测序，快递了4天了还没到测序公司，会不会坏掉啊    ( 1 2 ) (13/3140) jmh5d 2014-05-03 2014-05-06 10:44:08 by BioChen
[PCR相关] 为什么我用gDNA连一个3kb的片段都扩不出来？    ( 1 2 ) (评阅+1) (12/1902) Carnity 2014-04-29 2014-05-06 10:04:44 by 朱多龙
[PCR相关] [已完结]PCR产物回收胶的保存    ( 1 2 ) (15/2623) xielifenxie 2014-04-28 2014-05-05 19:01:08 by yanzhuo
[PCR相关] [已完结]gene库里面没有包含的种属---如何设计引物 (2/1096) kindbigbear0 2014-04-01 2014-05-05 13:08:53 by kindbigbear0
[PCR相关] [已完结]最近做pcr,内参出来了，目的片段却一直出不来，只有引物二聚体    ( 1 2 ) (14/2807) dingcong1216 2014-04-30 2014-05-05 09:47:34 by dingcong1216
[PCR相关] ChIP-QPCR (评阅+1) (8/4629) 宙钰乾坤 2014-04-29 2014-05-05 09:25:05 by yipan
[PCR相关] [已完结]16 S rDNA能否做内参 (评阅+1) (0/1439) qinghuaihulu 2014-05-04 2014-05-04 16:55:49 by qinghuaihulu
[PCR相关] [已完结]pcr出现类似弥散问题怎么回事，帮我分析分析原因，谢谢 (1/629) 意萧02 2014-05-04 2014-05-04 16:29:32 by 1057910381
[PCR相关] [已完结]在实时荧光PCR中参照基因是用25s rRNA还β-actin比较好？ (5/714) 小比目鲨鱼 2014-04-30 2014-05-04 15:39:47 by Neo2000
[PCR相关] [已完结][关贴]我犯了一个挺有意思的错误，大家进来讨论一下吧 (7/1292) wenzhipeng 2014-04-30 2014-05-04 15:39:38 by Asura-
[PCR相关] [已完结]qRT-PCR, 组织特异性表达 (评阅+1) (0/1277) 麦水螅 2014-05-04 2014-05-04 09:13:34 by 麦水螅
[PCR相关] [已完结]什么是PCR冷启动？ (2/2601) 半调子梅花 2014-05-03 2014-05-03 15:29:41 by 半调子梅花
[PCR相关] 请问巢式pcr的灵敏度大概有多少？ (2/1382) 昨日牛郎 2014-05-02 2014-05-03 12:20:43 by meng_xu
[PCR相关] [已完结]SSR转化SCAR 引物设计 (0/523) Botak 2014-05-02 2014-05-02 12:31:15 by Botak
[PCR相关] [已完结]ISSR-PCR标记电泳图 (6/1793) penny923 2014-04-01 2014-05-02 10:55:23 by timesty
[PCR相关] [已完结]琼脂糖凝胶电泳严重拖尾 (4/2116) sinky熹 2014-05-01 2014-05-02 09:56:54 by yuren2009
[PCR相关] [已完结]阳性克隆的鉴定 (5/1878) 崔永霞 2014-04-30 2014-05-01 12:29:03 by zep616745243
[PCR相关] [已完结]大家有没有遇到这种情况？ (4/962) 木vs木 2014-04-30 2014-05-01 10:18:23 by 西门吹雪170
[PCR相关] 有没有人遇到过跑胶时PCR产物会减少的现象，请大神们指教 (7/1599) houyuru1990 2014-04-30 2014-04-30 20:26:28 by pennchem
[PCR相关] [已完结]保真性太差，200多的片段有6-7个bp的错配    ( 1 2 3 4 ) (33/1944) leiqin 2014-04-27 2014-04-30 12:27:34 by shuifen1108
[PCR相关] [已完结]关于筛选htSNP。 (0/504) David勇 2014-04-30 2014-04-30 12:01:20 by David勇
[PCR相关] [已完结]下游引物设计求助 (1/1583) cassie小萍 2014-04-29 2014-04-29 18:11:54 by 西门吹雪170
[PCR相关] [已完结]PCR电泳图请帮忙分析    ( 1 2 ) (评阅+1) (10/1783) lumy790101 2014-04-23 2014-04-29 14:51:40 by lumy790101
[PCR相关] [关贴]求帮助 (2/352) guminghong 2014-04-29 2014-04-29 14:14:49 by Rubisco580
[PCR相关] [已完结]甲基化敏感性内切酶 (评阅+1) (MolEPI+1)(2/7604) r-rainbow 2014-04-29 2014-04-29 12:24:50 by biostar2009
[PCR相关] [已完结]PCR第一次扩出来第二次扩不出来 (7/1959) er66668888 2014-03-30 2014-04-29 11:27:06 by zhaoxiaopang
[PCR相关] [已完结]PCR，电泳 (评阅+1) (5/709) summit061800 2014-04-24 2014-04-29 10:09:48 by zhaoxiaopang
[PCR相关] 请问大家PCR扩增的最长产物是多少bp    ( 1 2 ) (10/5902) 283503264 2014-04-09 2014-04-29 09:41:20 by 283503264
[PCR相关] [已完结]有关是不是引物二聚体的分析    ( 1 2 ) (10/2273) 三蛰 2014-03-23 2014-04-28 23:58:33 by zep616745243
[PCR相关] [已完结]荧光定量 (评阅+1) (0/215) tuhua 2014-04-28 2014-04-28 21:53:34 by tuhua
[PCR相关] [已完结]实时定量PCR荧光值出现过早，但荧光值先稍微升高后又下降？ (2/1137) 大甫哥 2014-04-27 2014-04-28 19:35:16 by 大甫哥
[PCR相关] [已完结]最近测了1700 bp的一段序列，用的是PCR产物，结果是前后40 bp左右没测出来 (4/957) liuyil 2014-04-27 2014-04-28 16:59:42 by 青苹果QT
[PCR相关] [已完结]PCR产物测序 (2/457) 呀薯条 2014-04-28 2014-04-28 13:34:38 by zep616745243
[PCR相关] [已完结]荧光定量PCR (8/894) xiayu608 2014-04-24 2014-04-28 10:09:15 by 邹忌
[PCR相关] [已完结]用cdna跑不出目的条带，反而用dna跑出了目的条带，实在搞不懂了 (3/2192) 想学好的学渣 2014-04-27 2014-04-28 09:57:25 by 想学好的学渣
[PCR相关] [已完结]我的条带小于目的条带。。。求助。。。。    ( 1 2 ) (15/2471) hhl1230 2014-04-23 2014-04-27 19:57:29 by Hliotrope
[PCR相关] [已完结]全长cDNA PCR问题 (评阅+1) (4/958) ashleyoo00 2014-04-26 2014-04-27 10:54:44 by ashleyoo00
[PCR相关] [已完结]查找启动子 (评阅+1) (2/2204) 花花NO.1 2014-04-24 2014-04-26 22:29:48 by 凌波丽
[PCR相关] [已完结]英雄，HELP！ 这个基因死活扩不出来啊 (1/741) KEVIN_LOVE 2014-04-25 2014-04-26 16:39:28 by br24
[PCR相关] [已完结]RT-PCR (3/904) 伊梦青lhq 2014-04-25 2014-04-26 12:35:28 by 伊梦青lhq
[PCR相关] 噬菌体展示 pcr 没带 什么原因 (1/1387) linyingjia 2014-04-25 2014-04-26 09:54:32 by 凌波丽
[PCR相关] [已完结]Nde I 和 Not I 可以一起用吗？ (3/1293) 小迷糊912 2014-04-23 2014-04-25 18:58:53 by 小迷糊912
[PCR相关] [已完结]DGGE实验动物样本本身个体差异大怎么办 (0/805) 小虫5903 2014-04-25 2014-04-25 10:53:13 by 小虫5903
[PCR相关] [已完结]转化后平板不长菌    ( 1 2 ) (19/3762) 最末那抹夕阳 2014-04-21 2014-04-25 01:18:11 by jukongka
[PCR相关] [已完结]荧光定量检测不到信号 (2/1328) 熙玥 2014-04-24 2014-04-24 23:15:49 by 熙玥
[PCR相关] PCR引物是选择特异性引物还是oligo (0/2333) suzhou1989 2014-04-24 2014-04-24 16:25:36 by suzhou1989
[PCR相关] [已完结]SSR引物筛选 (0/1322) penny923 2014-04-24 2014-04-24 13:45:10 by penny923
[PCR相关] [已完结]荧光定量PCR (5/722) xiayu608 2014-04-23 2014-04-24 10:59:22 by jurkat.1640
[PCR相关] [已完结]做real time PCR时为什么不能先把DNA都加进去再分装 (5/792) crazymouse66 2014-04-23 2014-04-24 10:13:19 by cituyuqi
[PCR相关] [已完结]菌液PCR验证 (评阅+1) (7/929) wanghf_6134 2014-03-30 2014-04-24 08:35:23 by XOooZzz
[PCR相关] [已完结]大家认为判断引物设计合理的主要依据有哪些 (评阅+1) (7/4231) 简单的执着 2014-04-21 2014-04-23 23:41:24 by gyesang
[PCR相关] [已完结]做真菌（最好是曲霉类）siRNA或miRNA PCR检测时的内参基因大家都用的什么？ (评阅+1) (0/1673) bbyu007 2014-04-23 2014-04-23 17:33:08 by bbyu007
[PCR相关] [已完结]大侠帮我看看，这个嵌合体检测结果怎么看啊，没做过啊 (0/3687) cya_yanan 2014-04-23 2014-04-23 15:33:03 by cya_yanan
[PCR相关] [已完结]做转化时，感受态用多大的量比较好呢    ( 1 2 ) (15/2782) 柠檬可乐星 2014-04-17 2014-04-23 14:31:12 by 柠檬可乐星
[PCR相关] [已完结]如何确定自己的目的片段 PCR 痛苦中    ( 1 2 ) (17/2886) wanglixia815 2014-04-20 2014-04-23 13:34:37 by 凌枫miracle
[PCR相关] [已完结]RT- PCR 跑出来的带有几条，求助 (评阅+1) (6/1077) 唐萌2 2014-04-15 2014-04-23 00:51:15 by xygcdmr
[PCR相关] [已完结]PCR一直p不出来，求大神指点（for DGGE） (2/646) xhy315 2014-04-10 2014-04-22 19:50:04 by yinlizhangli
[PCR相关] [已完结]求大神分析一下这次的PCR结果 (评阅+1) (1/317) 冰糖-葫芦娃 2014-04-16 2014-04-22 15:21:20 by 邹忌
[PCR相关] [已完结]分析电泳图，求神人过来啊~~救命啊~~！！！指点啊    ( 1 2 ) (10/1402) 小小鱼泪了 2014-04-21 2014-04-22 13:29:49 by jiaolong11a
[PCR相关] [已完结]用链霉菌DNA做荧光定量PCR，相对定量？ (评阅+1) (0/428) benita-chen 2014-04-22 2014-04-22 10:42:31 by benita-chen
[PCR相关] [已完结]相关引物 (0/232) wm阳光 2014-04-21 2014-04-21 20:38:11 by wm阳光
[PCR相关] [已完结]已经快PCR折磨疯了。。救命呀    ( 1 2 3 4 5 ) (评阅+1) (41/2477) wanglixia815 2014-04-09 2014-04-21 17:30:07 by lsg-ll
[PCR相关] [已完结]麻烦各位看一下我溶解曲线的结果 (1/1373) longyuming 2014-04-20 2014-04-21 15:50:14 by zhangyunjing
[PCR相关] [已完结]扩增质粒的技术方法 (1/825) 发酵与酶工程 2014-04-20 2014-04-20 23:49:31 by huangrui1988
[PCR相关] 连接、转化以及酶切问题 (评阅+1) (0/579) 蜜桃椰果 2014-04-20 2014-04-20 18:35:39 by 蜜桃椰果
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