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[热点] 售SCI一区文章，我:8.O.55.1.O.54,科目齐全,可伽急 ncTVBM6AOJHF 2026-11-05 刚刚
[PCR相关] [已完结]GCbuffer (2/2194) berry94 2014-06-07 2014-06-07 18:59:57 by berry94
[PCR相关] [已完结]大家做定量PCR时都用多大的稀释倍数啊？ (4/5818) lm334723460 2014-06-06 2014-06-07 16:51:50 by chqu
[PCR相关] [已完结]7kb质粒定点突变PCR检测无产物还要继续做下去吗 (3/1371) 艰苦温度 2014-06-06 2014-06-07 09:13:02 by huangrui1988
[PCR相关] [已完结]为什么我用primer设计的引物评分都这么低呀 (评阅+1) (3/3947) isaiah2012 2014-06-05 2014-06-06 17:26:45 by Neo2000
[PCR相关] [已完结]RT-PCR过程中内参基因CT值问题 (6/8167) 秋隐vs蚯蚓 2014-06-04 2014-06-06 16:23:54 by ybQuan
[PCR相关] [已完结]请问一下ABI7500Fast的仪器必须要用ABI的八连管吗？ (评阅+1) (2/4095) leicandy 2014-06-05 2014-06-05 23:30:11 by zhuangkai520
[PCR相关] [已完结]pCDNA3.1载体中Kozak序列的问题 (1/4664) KEVIN_LOVE 2014-06-05 2014-06-05 15:11:06 by yipan
[PCR相关] [已完结]求助关于pcr拖带的问题 (评阅+1) (3/1456) Dylan苏木樨 2014-06-05 2014-06-05 14:42:03 by grase
[PCR相关] [已完结]跑电泳，凝胶上什么都没有，连溴酚蓝都没有 (评阅+1) (4/2002) 斯小乔 2014-06-04 2014-06-05 11:26:27 by hutil1543
[PCR相关] [已完结]RAPD分子标记 (4/1012) 心月之星 2014-05-27 2014-06-05 11:09:09 by 心月之星
[PCR相关] [已完结]提取的细菌DNA，准备做PCR，怎么确定提取的DNA菌浓度呢？ (评阅+1) (1/1646) qureen 2014-06-04 2014-06-05 05:43:17 by meng_xu
[PCR相关] 跟大家交流一下关于荧光定量PCR实验的问题 (评阅+1) (3/961) leicandy 2014-06-03 2014-06-04 16:57:36 by 成功源于想像
[PCR相关] 类免疫球蛋白基因的3'-UTR最长大概有多长？ (评阅+1) (0/1622) kkxly 2014-06-04 2014-06-04 16:09:16 by kkxly
[PCR相关] [已完结]做过反向PCR的筒子进来看一下吧    ( 1 2 ) (评阅+1) (14/2000) shellyxss 2014-06-01 2014-06-04 08:56:02 by shellyxss
[PCR相关] [已完结]RT-PCR 疑难杂症求助！ (评阅+1) (1/1102) baobaozhu 2014-06-02 2014-06-04 08:23:41 by baobaozhu
[PCR相关] [已完结]DGGE中用条带亮度不能代表菌株是否是优势菌吗？生物侠们来瞅瞅~ (8/1190) jinzhan991 2014-05-30 2014-06-03 14:48:13 by jinzhan991
[PCR相关] [已完结]荧光定量Pcr问题求助 (7/2496) 好好后生 2014-05-31 2014-06-03 08:43:03 by jurkat.1640
[PCR相关] [已完结]重金求助啊！谁能给俺设计几个引物，并预测一下反应条件？    ( 1 2 3 ) (23/2556) dswjhs 2014-05-20 2014-06-03 07:43:48 by jelly1106
[PCR相关] [已完结]染色体步移相关问题 (0/291) 潇潇杨雨125 2014-06-03 2014-06-03 00:12:27 by 潇潇杨雨125
[PCR相关] [已完结]为什么我150ul的体系，分成3管50ul PCR，有一管出来目的条带    ( 1 2 ) (14/2107) cao云中歌 2014-05-27 2014-06-01 21:32:44 by sarah-miao
[PCR相关] [已完结]HNF4a的引物及退火温度？？？人源和鼠源 (评阅+1) (0/496) loulou许 2014-05-31 2014-05-31 12:15:33 by loulou许
[PCR相关] [已完结]PCR结果不稳定 (4/2099) karuirui 2014-05-29 2014-05-30 14:48:05 by karuirui
[PCR相关] [已完结]测序完之后，怎么看测序结果？请指教 (评阅+1) (6/4483) wanglixia815 2014-05-27 2014-05-30 14:43:38 by wanglixia815
[PCR相关] [已完结]克隆基因，总是少28bp    ( 1 2 3 ) (23/3366) 一桶稀泥 2014-05-27 2014-05-30 09:22:43 by cuijinmiss
[PCR相关] [已完结]5'RACE测序结果 (评阅+1) (0/877) 魔术师杨 2014-05-30 2014-05-30 08:30:32 by 魔术师杨
[PCR相关] [已完结]RACE实验 (3/698) shiliyusly 2014-05-29 2014-05-29 20:11:37 by Neo2000
[PCR相关] [已完结]想请教一下关于荧光定量PCR的问题 (评阅+1) (2/546) leicandy 2014-05-28 2014-05-29 17:29:25 by leicandy
[PCR相关] [已完结]叶绿体trnL-trnF片段怎么扩出700多bp了？ (评阅+1) (2/635) 娅卜 2014-05-26 2014-05-29 15:05:18 by 娅卜
[PCR相关] [已完结]罗氏480没有ROX通道? (2/4059) LXD_0001 2014-05-27 2014-05-29 11:37:05 by silicare
[PCR相关] [已完结]HBV DNA检测试剂盒主要扩增的是哪段序列 (0/215) sd6372335 2014-05-29 2014-05-29 11:36:16 by sd6372335
[PCR相关] [已完结]荧光定量RT-PCR结果求分析！ (评阅+1) (4/2283) linsui1989 2014-05-26 2014-05-29 11:31:24 by vege的桌子
[PCR相关] [已完结]5’RACE结果对不上，求帮助，必有重谢!! (7/2050) 梦的彩虹 2014-05-22 2014-05-29 10:48:21 by 梦的彩虹
[PCR相关] [已完结]关于实时荧光定量PCR (1/713) 宏伟10 2014-05-28 2014-05-28 15:32:34 by 飞约疯人院
[PCR相关] [已完结]一种方便的切胶小工具 (评阅+1) (6/1399) LadyMoss 2014-05-26 2014-05-28 12:54:12 by LadyMoss
[PCR相关] [已完结]QPCR数据分析 (评阅+1) (0/708) sxzxs 2014-05-28 2014-05-28 12:19:23 by sxzxs
[PCR相关] [已完结]请问NCBI中query cover表示什么意义 (2/27263) 美好的萝卜 2014-05-27 2014-05-28 12:12:44 by 飞约疯人院
[PCR相关] 兄弟姐妹们来帮我看下根据这几个线索能否确定一个pcr产物是否为我要的片段 (评阅+1) (1/853) 新世界D共鸣 2014-05-28 2014-05-28 11:26:01 by 新世界D共鸣
[PCR相关] [已完结]RACE测序结果 (6/1417) 魔术师杨 2014-05-27 2014-05-28 09:02:43 by 魔术师杨
[PCR相关] [已完结]请教一下关于引物的保存 (3/1177) leicandy 2014-05-27 2014-05-27 21:56:24 by 小月亮1990
[PCR相关] [已完结]PCR中简并引物量的优化 (评阅+1) (2/782) cherish美丽 2014-05-27 2014-05-27 21:49:03 by 飞约疯人院
[PCR相关] [已完结]PCR酶切位点的保护碱基可以随意设置吗？ (评阅+1) (6/5332) 108391 2014-05-26 2014-05-27 20:01:44 by 108391
[PCR相关] [已完结]请大家帮忙，p不出条带 (2/684) 泡菜肉丝 2014-05-27 2014-05-27 18:49:32 by 泡菜肉丝
[PCR相关] [已完结]3’端race菌落PCR体系的问题 (3/975) 冰糖-葫芦娃 2014-05-27 2014-05-27 17:10:36 by 飞约疯人院
[PCR相关] [已完结]请问一下关于real-time PCR的问题 (评阅+1) (2/516) leicandy 2014-05-26 2014-05-27 16:51:48 by jurkat.1640
[PCR相关] [已完结]急！请教荧光定量，急，谢谢大家！ (0/467) wzc4884 2014-05-27 2014-05-27 11:01:53 by wzc4884
[PCR相关] [已完结]pcr的产物有吗？（菜鸟求教大神） (5/1588) yuliang13 2014-05-25 2014-05-26 22:42:19 by ghqiu09
[PCR相关] [已完结]请教植物激素处理下测定基因实时表达的问题 (评阅+1) (0/489) okletsgo 2014-05-26 2014-05-26 17:15:15 by okletsgo
[PCR相关] [已完结]RNA提取和RT-PCR (评阅+1) (1/438) 970402570 2014-05-26 2014-05-26 17:01:26 by Neo2000
[PCR相关] [已完结]要鉴定菌种时做PCR的褪火温度 (评阅+1) (1/488) 小石头s 2014-05-26 2014-05-26 17:00:30 by DAX1
[PCR相关] [已完结]3’端race菌落PCR体系的问题 (1/386) 冰糖-葫芦娃 2014-05-21 2014-05-26 13:36:29 by 冰糖-葫芦娃
[PCR相关] [已完结]基因克隆 (评阅+1) (9/1551) 红卫大兵 2014-05-24 2014-05-26 10:33:21 by 红卫大兵
[PCR相关] [已完结]PCR 克隆 问题，小弟新手，求帮助。 (评阅+1) (7/1740) zlimba 2014-04-22 2014-05-26 09:12:16 by dizang2
[PCR相关] [已完结]miRNA和lncRNA的提取 (评阅+1) (1/3270) sweetqm 2014-05-24 2014-05-25 19:23:22 by biostar2009
[PCR相关] [已完结]扩增效率 (1/1356) lanlvmaomao 2014-05-25 2014-05-25 02:07:23 by houliming
[PCR相关] [已完结]荧光定量pcr重复性问题 (评阅+1) (5/4154) lanlvmaomao 2014-05-08 2014-05-25 01:24:57 by lanlvmaomao
[PCR相关] [已完结]引物设计跨内含子 (6/6120) 061719 2014-05-15 2014-05-24 23:39:51 by Renjingyu
[PCR相关] [已完结]5‘RACE (5/636) 魔术师杨 2014-05-19 2014-05-24 21:56:48 by 梦的彩虹
[PCR相关] [已完结]测序结果引物部分跟原本应该的序列相比少个碱基，是引物的问题还是连接过程中丢失？ (3/3292) love犬夜叉 2014-05-22 2014-05-24 19:01:12 by BioChen
[PCR相关] [已完结]加酶切位点 (评阅+1) (3/999) 红卫大兵 2014-05-24 2014-05-24 13:21:49 by stone2239
[PCR相关] [已完结]巢式PCR测序结果分析 (评阅+1) (2/754) nieqiantina 2014-05-24 2014-05-24 11:33:28 by nieqiantina
[PCR相关] [已完结]荧光定量PCR实验 求助    ( 1 2 ) (12/1948) 忘记将来 2014-05-15 2014-05-23 16:43:26 by 忘记将来
[PCR相关] [已完结]miRNA 上游引物 (2/746) 明德2013 2014-04-11 2014-05-23 16:37:33 by dingxianlo
[PCR相关] [已完结]荧光定量PCR可以看到产物大小吗？ (评阅+1) (6/2774) 永远一片天 2014-05-17 2014-05-23 16:28:50 by wangweijian
[PCR相关] [已完结]请问什么是Liquid-array PCR方法？ (评阅+1) (1/538) 永远一片天 2014-05-23 2014-05-23 15:57:21 by 小四当当
[PCR相关] [已完结]荧光定量标准曲线问题！！急急急！在线等答案 (评阅+1) (3/1386) 学术问题 2014-05-11 2014-05-23 15:14:38 by 学术问题
[PCR相关] [已完结]求助鉴定突变体条带的问题 (3/786) 大懒猫很懒 2014-05-23 2014-05-23 14:29:15 by 大懒猫很懒
[PCR相关] [已完结]【求助】哪位学长学姐给分析一下这张琼脂糖凝胶电泳图的结论 (1/991) 我心永恒天行 2014-05-23 2014-05-23 13:59:23 by 我心永恒天行
[PCR相关] [已完结]PCR扩不出条带 (1/557) 博克侬浮码 2014-05-23 2014-05-23 11:14:55 by HYZ221314
[PCR相关] [已完结]扩增产物比欲扩增的少了600bp，就解释 (0/443) dai1103 2014-05-23 2014-05-23 10:38:28 by dai1103
[PCR相关] [已完结][关贴]跪求PCR大神，解决小弟问题，都一个多月了，快被折腾死了 (评阅+1) (7/2127) ?木木@ 2014-05-20 2014-05-23 10:10:16 by yuefeng506
[PCR相关] [已完结]新手做荧光定量PCR几个疑问求解答！！    ( 1 2 ) (评阅+5) (13/3164) 梦梦神 2014-05-17 2014-05-23 09:50:09 by 梦梦神
[PCR相关] [已完结]菌液pcr呈阳性，但是提取的重组质粒差不多10000bp, (1/791) 生科廊 2014-05-22 2014-05-23 05:54:39 by meng_xu
[PCR相关] [已完结]活化后的菌液-20保存一周，pcr直接用可以么 (评阅+1) (4/1371) friedy 2014-05-19 2014-05-23 05:49:02 by meng_xu
[PCR相关] [已完结]分子生物 荧光定量PCR (1/424) sunleixy 2014-05-22 2014-05-22 22:45:32 by Lau_Kimura
[PCR相关] [关贴]请教 (4/802) guminghong 2014-05-21 2014-05-22 21:59:16 by guminghong
[PCR相关] [已完结]怎样确定一个已知基因两侧的未知序列 (7/2537) 预防凯凯 2014-05-17 2014-05-22 19:50:43 by 夏天的普洱茶
[PCR相关] [已完结][关贴]求好心人帮帮我啊！ (0/339) laozake 2014-05-22 2014-05-22 19:36:32 by laozake
[PCR相关] [已完结]请问我全长基因跑出来之后为什么要小100bp左右呢？ (9/2587) louisjackson 2014-05-08 2014-05-22 17:39:19 by mehongyu
[PCR相关] [已完结]荧光定量引物设计问题 (3/844) xiehuiling 2014-05-21 2014-05-22 15:07:24 by xiehuiling
[PCR相关] [已完结]pcr之后跑琼脂糖 优化，最后要跑聚丙烯酰胺的，希望大家给个优化方向，谢谢！    ( 1 2 3 ) (21/1309) 吉部落zy 2014-05-17 2014-05-22 11:05:08 by 吉部落zy
[PCR相关] [已完结]这样一段基因该如何调取，请指教，谢谢 (3/783) q0cheng 2014-05-21 2014-05-22 10:38:02 by 夏天的普洱茶
[PCR相关] [已完结]载体构建，测序结果显示目的基因全突变，克隆入T载体，菌液PCR全是假阳性    ( 1 2 ) (评阅+1) (11/4613) liuwhlong 2014-04-30 2014-05-21 22:47:55 by liuwhlong
[PCR相关] [已完结]简并引物ΔG的绝对值怎么算？？？ (评阅+1) (1/1175) sertying 2014-05-21 2014-05-21 21:12:58 by wpwupingwp
[PCR相关] [已完结]设计引物时正向引物和反向引物的3’端后面的四个碱基相同这样可以吗，求大神指导~ (2/3475) 梦的彩虹 2014-05-21 2014-05-21 20:33:11 by yuren2009
[PCR相关] [已完结]半定量PCR (评阅+1) (1/629) 80后的末班车 2014-05-21 2014-05-21 16:56:42 by 冷雁孤旅
[PCR相关] [已完结]构建系统发育树 (0/566) a1996630wo 2014-05-21 2014-05-21 16:09:23 by a1996630wo
[PCR相关] [已完结]关于热启动的修饰方法的比较 (0/838) zhangyunjing 2014-05-20 2014-05-20 15:04:56 by zhangyunjing
[PCR相关] 大家看看我提的质粒，最前面是不是RNA污染啊，该怎么避免啊？ (3/5162) guminghong 2014-05-17 2014-05-20 09:43:48 by 刘芬玲
[PCR相关] [关贴]请教 (3/375) pissor 2014-05-20 2014-05-20 02:12:21 by yuguiyan
[PCR相关] [已完结]荧光定量PCR产物 (0/527) kekebing 2014-05-19 2014-05-19 16:05:38 by kekebing
[PCR相关] [已完结]菜鸟求教，melt curve stage是什么意思啊 (4/7530) qinmengnan 2014-05-15 2014-05-19 13:45:22 by 眼泪笑了-rui
[PCR相关] [已完结]请教各位关于structure软件的问题，不胜感激! (评阅+1) (1/848) yedeleng 2014-04-28 2014-05-19 13:17:58 by yedeleng
[PCR相关] [已完结]关于biowest agarose 用哪个厂家分装的好？ (1/472) liqian901117 2014-05-19 2014-05-19 12:27:12 by junboliang
[PCR相关] [已完结]PCR相关 (评阅+1) (0/261) jintao22058 2014-05-19 2014-05-19 11:23:33 by jintao22058
[PCR相关] [已完结]荧光定量 (评阅+1) (0/336) tuhua 2014-05-17 2014-05-17 16:37:49 by tuhua
[PCR相关] [已完结]PBI121表达载体替换35s后测序的问题 (0/730) lucq5577 2014-05-17 2014-05-17 12:48:26 by lucq5577
[PCR相关] [已完结]RACE实验批出全长，用高保真酶验证序列， (0/496) 自由的淅沥 2014-05-17 2014-05-17 09:49:25 by 自由的淅沥
[PCR相关] [已完结]问问各位大哥大姐，我用正交试验设计做的一个PCR条件优化，该如何挑选最优的组合？ (6/1602) mojx 2014-05-08 2014-05-16 19:13:25 by mojx
[PCR相关] 有没有用pESC相关的质粒的，贵不贵啊？ (0/282) xutianhuizi 2014-05-16 2014-05-16 17:01:48 by xutianhuizi
[PCR相关] [已完结]克隆5KB左右的基因，两个多月了还没进展，求帮忙啊！！！！    ( 1 2 3 ) (21/2373) 米粒儿308 2014-05-13 2014-05-16 16:50:35 by yuefeng506
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