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[热点] 各位基础老师们，看完中央《从严治党》的决定后，对学术界更加有信心了吧 iaeyuan 2026-11-05 刚刚
[PCR相关] [已完结]请大神帮分析下测序结果拜托了 (1/458) 19910204 2015-01-27 2015-01-27 10:18:19 by 781055707
[PCR相关] 合成的单链DNA作为扩增子，产物有非目的条带 (4/1138) wodejiapple 2015-01-24 2015-01-27 10:13:04 by 18761615793
[PCR相关] [已完结]如何鉴定一种DNA样本中的不同样本类型所占百分比    ( 1 2 ) (评阅+1) (17/2661) linzshit 2014-07-28 2015-01-27 06:15:58 by maweisimple
[PCR相关] [已完结]q-PCR连续实验，发现同一样品、同一基因ct值呈等差数列下降 (评阅+1) (5/1061) sujiyan 2015-01-24 2015-01-27 00:15:56 by sujiyan
[PCR相关] [已完结]求 蛋白转录组 测序相关的文献。 (2/484) wlf284173066 2014-12-10 2015-01-26 20:38:11 by 飞约疯人院
[PCR相关] [已完结]RT-PCR相关问题，新手遇到一些问题，比较纠结，前来求教？ (2/658) 踏雪无极限 2014-11-24 2015-01-26 18:47:11 by 陆朝军
[PCR相关] [已完结]qpcr溶解曲线问题 (1/1862) 1291115202 2015-01-26 2015-01-26 17:28:07 by 飞约疯人院
[PCR相关] [已完结]说我遇到了罕见事件，大家帮我分析分析 (4/775) msw0103 2015-01-26 2015-01-26 17:21:50 by zshw_001
[PCR相关] [已完结]筛选阳性克隆    ( 1 2 3 ) (20/2925) yuman0709 2015-01-23 2015-01-26 12:22:05 by zhangtao110
[PCR相关] 关于融合PCR (评阅+1) (7/2034) 兰舍难分 2015-01-19 2015-01-26 10:50:42 by 18761615793
[PCR相关] 荧光定量PCR可以直接用提取的基因组DNA作为模板吗？ (5/6702) sophia_p 2015-01-13 2015-01-25 20:30:51 by 清雅风
[PCR相关] [已完结]重组质粒转到BL21（DE3）中用T7检测没条带，用特异引物检测有条带，怎么回事    ( 1 2 ) (评阅+1) (15/2585) ginnyzhuzhu 2015-01-21 2015-01-25 11:41:22 by ginnyzhuzhu
[PCR相关] 关于分子诊断试剂盒常遇到的概率问题    ( 1 2 3 ) (24/2097) huangchj03 2015-01-24 2015-01-25 09:34:12 by dragon1971
[PCR相关] [已完结]请问可以设计转录因子和DNA的结合位点设计引物做ChIP呢？ (评阅+1) (0/1426) xiaofengyyy 2015-01-24 2015-01-24 19:33:41 by xiaofengyyy
[PCR相关] [已完结]2.3kb 大片段pcr 求赐教    ( 1 2 3 4 ) (32/2792) hailey 2014-06-02 2015-01-24 17:34:37 by 艰苦温度
[PCR相关] 有谁用过Thermo scientifc 公司的RevertAid™第一链cDNA Synthesis试剂盒？ (评阅+1) (0/472) 名白纸号白纸 2015-01-24 2015-01-24 17:21:38 by 名白纸号白纸
[PCR相关] [已完结]植物单细胞pcr (2/460) jz1176986189 2015-01-23 2015-01-24 10:02:19 by jz1176986189
[PCR相关] 长片段DNA扩增后电泳挂孔问题 (评阅+1) (8/4393) 李袁硕 2015-01-21 2015-01-23 23:44:59 by 稻稻
[PCR相关] [已完结]用LATaq酶PCR的产物电泳条带拖带，能切胶回收做TA克隆吗？ (评阅+1) (5/2991) xiangqin90 2015-01-22 2015-01-23 10:09:55 by 18761615793
[PCR相关] [已完结]多重PCR鉴定大肠杆菌用哪个基因？ (0/1678) jingmi 2015-01-22 2015-01-22 15:37:43 by jingmi
[PCR相关] [已完结]半定量RT-RNA中怎么克隆内参基因18S rRNA (评阅+1) (2/1804) lu12306 2014-12-08 2015-01-22 15:12:44 by lu12306
[PCR相关] 空载体双酶切后就没有了 (评阅+1) (4/1310) musegongxi 2015-01-21 2015-01-22 14:42:18 by musegongxi
[PCR相关] HRM可以使用兼并引物吗 (评阅+1) (2/623) fairmoon 2015-01-22 2015-01-22 12:21:16 by titus0805
[PCR相关] [已完结]农杆菌GV3101或者LBA4404 (1/1571) lijianfu12 2015-01-22 2015-01-22 05:45:39 by 小小cool
[PCR相关] [已完结]GLA基因引物设计问题 (评阅+1) (0/717) 977673882 2015-01-21 2015-01-21 19:25:59 by 977673882
[PCR相关] [已完结]QPCR引物设计 (评阅+1) (5/955) 1291115202 2015-01-21 2015-01-21 17:34:52 by zshw_001
[PCR相关] [已完结]定量PCR    ( 1 2 ) (评阅+1) (17/1751) 悠悠飘杨 2015-01-15 2015-01-21 16:29:44 by txing
[PCR相关] [已完结]taqman探针的荧光基团和淬灭基团的问题 (评阅+1) (2/7134) huangchj03 2015-01-21 2015-01-21 12:09:39 by huangchj03
[PCR相关] [已完结]引物设计求助 (1/664) hanbing7525 2015-01-20 2015-01-21 09:37:00 by chenguestc
[PCR相关] [已完结]大家帮我分析一下qpcr结果 (4/2299) 舟之行 2014-09-22 2015-01-21 08:49:11 by huangchj03
[PCR相关] [已完结]求助，NCBI设计的引物怎么看好不好？急呀。。。。。 (5/19226) 雪花爱上梅花 2014-03-13 2015-01-21 07:06:15 by lvbobo823
[PCR相关] 自产KOD酶pcr (12/1905) zhoubin0823 2015-01-20 2015-01-20 19:26:02 by 小小cool
[PCR相关] [已完结]5"race 技术识别转录起始位点的原理是什么？ (评阅+1) (4/3908) 绿筱一夏 2015-01-19 2015-01-20 15:40:42 by titus0805
[PCR相关] [已完结]我的cDNA.你在哪，怎么千呼万唤不出来    ( 1 2 3 4 ) (31/2552) 涅槃之曦 2015-01-15 2015-01-20 15:30:56 by 疯狂毒理学
[PCR相关] [已完结]重组PCR 扩不出来需要的条带 (3/1062) jjsh310 2014-11-25 2015-01-20 11:13:37 by 100421
[PCR相关] [已完结]ISSR-PCR分析时每一种不同材料都得进行体系优化吗，到底和哪些因素有关？    ( 1 2 3 4 ) (38/2491) 867575969 2014-04-25 2015-01-20 07:59:12 by 123lp456
[PCR相关] [已完结]凝胶电泳的拖尾现象 (评阅+1) (6/2001) liruiyun 2015-01-19 2015-01-19 20:41:21 by 18761615793
[PCR相关] [已完结]常规PCR条带很亮，荧光定量pcr无扩增曲线求解！ (评阅+1) (2/1411) 东方亦雪 2015-01-19 2015-01-19 17:59:20 by 红卫大兵
[PCR相关] [已完结][关贴]两个基因的5'和3'端序列相同长度也相同，构建载体时如何区分开二者？ (1/985) liumeng611 2015-01-19 2015-01-19 16:24:48 by liuderong
[PCR相关] 为什么没有条带 (4/839) 蓝色木槿 2015-01-16 2015-01-19 16:00:33 by 雨无论次
[PCR相关] [已完结]有人了解 two-tiered PCR method吗~求经验交流~ (评阅+1) (5/1085) 低调绅狮 2015-01-09 2015-01-19 13:09:52 by 18761615793
[PCR相关] [已完结]HLA-DQB1等位基因的分型 (1/741) zhangyunjing 2014-08-11 2015-01-19 12:06:49 by lulijun2014
[PCR相关] [已完结]如何用e-pcr检测已知的甘蓝型油菜ssr标记在甘蓝基因组是否存在 (0/260) hoajie 2015-01-19 2015-01-19 11:10:30 by hoajie
[PCR相关] 已知unigene，求帮忙设计一对荧光定量PCR引物 (评阅+1) (0/730) jiebingzh 2015-01-18 2015-01-18 20:03:04 by jiebingzh
[PCR相关] [已完结]pcr 产物在跑电泳时不出条带 (8/2736) 舟之行 2014-09-13 2015-01-18 16:31:28 by 舟之行
[PCR相关] Taq酶储藏问题 (评阅+1) (4/2921) qll8384 2015-01-16 2015-01-18 16:13:19 by qll8384
[PCR相关] [已完结]PCR反应体系改变会导致电泳不出带吗 (评阅+1) (6/1545) 小朱大宝 2014-10-20 2015-01-18 13:51:14 by yelon315
[PCR相关] [已完结]半定量一个组织PCR做不出来，求助    ( 1 2 ) (评阅+1) (13/2614) supersky3195 2015-01-15 2015-01-18 12:48:49 by lu12306
[PCR相关] 求助，凝胶成像照片gsd格式问题 (评阅+1) (2/852) beibei518 2015-01-17 2015-01-18 10:14:16 by beibei518
[PCR相关] [已完结]求助关于番茄TY3标记（P6-25）PCR检测所遇到的奇怪问题！！！ (评阅+1) (6/1405) 昺昺 2015-01-16 2015-01-17 22:03:28 by 昺昺
[PCR相关] [已完结]谁知道我这个PCR怎么是这样的啊？该如何调整？    ( 1 2 ) (评阅+1) (10/1868) tianti_121 2014-12-25 2015-01-17 12:54:28 by fry000
[PCR相关] [已完结]PCR为什么第二次就不出条带 (5/1699) 蓝色木槿 2015-01-15 2015-01-17 09:32:02 by 我是钢镚
[PCR相关] [已完结]求助：pcr 10微升体系跑出条带很亮，50微升体系跑不出东西 (4/3784) 守护13的爱恋 2014-04-23 2015-01-16 17:50:30 by 1009238055
[PCR相关] [已完结]BLAST 一对引物，怎么搜到一对引物都配对的？ (评阅+1) (4/2178) jingmi 2015-01-15 2015-01-16 11:09:01 by ptttmt
[PCR相关] PCR问题 (8/1064) qll8384 2015-01-13 2015-01-16 10:15:48 by qll8384
[PCR相关] [已完结]高GC含量目的基因的克隆    ( 1 2 3 ) (评阅+1) (23/3918) berry94 2015-01-04 2015-01-16 10:04:01 by 快乐农夫
[PCR相关] [已完结]转化子送去测序失败？？？    ( 1 2 ) (评阅+1) (10/1848) 小比目鲨鱼 2015-01-14 2015-01-16 09:57:36 by ptttmt
[PCR相关] [已完结]荧光定量PCR (评阅+1) (2/720) disapire 2015-01-15 2015-01-16 09:22:34 by lt822000
[PCR相关] [已完结]定量PCR (评阅+1) (2/1032) 悠悠飘杨 2015-01-15 2015-01-16 08:52:19 by 悠悠飘杨
[PCR相关] [已完结]Msp I 和Hha I 双酶切后200bp下的挑带没有跑开 (5/760) moqigang 2015-01-14 2015-01-15 23:21:05 by cicelyzh
[PCR相关] [已完结]做PCR-RFLP，用什么方法可以把电泳图谱分类OTU (评阅+1) (0/238) moqigang 2015-01-15 2015-01-15 19:56:28 by moqigang
[PCR相关] [已完结]PRIMER5.0软件求助 (评阅+1) (1/983) mtyx 2015-01-05 2015-01-15 14:37:08 by Gin—红与黑
[PCR相关] [已完结]大分子量蛋白怎样分段表达 (2/2380) 学员SYcRJB 2015-01-13 2015-01-15 12:47:42 by ptttmt
[PCR相关] [已完结]求一段不含ATG的DNA片段 400bp以上均可 (3/717) 逆天打酱油 2015-01-13 2015-01-15 12:22:59 by ptttmt
[PCR相关] 荧光PCR内参 (评阅+1) (0/925) Donnawang 2015-01-15 2015-01-15 10:52:22 by Donnawang
[PCR相关] [已完结]GUS瞬时表达 (评阅+1) (0/1141) 小朱大宝 2015-01-14 2015-01-14 15:02:00 by 小朱大宝
[PCR相关] [已完结]PCR条带淡，怎样优化？（如图）    ( 1 2 3 ) (24/3699) tianti_121 2014-12-28 2015-01-14 13:46:47 by molizerd
[PCR相关] 提取土壤总DNA跑胶没条带 求助 (8/2409) 黄太帝 2015-01-12 2015-01-13 22:43:25 by coolale
[PCR相关] [已完结]做小麦优质亚基Dx5，PCR后，检测不出来不知为什么？ (评阅+1) (4/1170) waiting_over 2015-01-11 2015-01-13 22:18:18 by waiting_over
[PCR相关] 多重PCR产物纯化回收后可以直接拿去测序吗？ (评阅+1) (3/1703) sophia_p 2015-01-07 2015-01-13 19:23:16 by sophia_p
[PCR相关] [已完结]核心序列为单核苷酸的SSR标记为啥一般不用 (9/2112) mungbean3881 2015-01-04 2015-01-13 09:33:35 by mungbean3881
[PCR相关] [已完结]怎样找取人CYP3A4上游12kb的启动序列 (评阅+5) (3/557) 风生竹动 2015-01-12 2015-01-13 09:16:03 by 风生竹动
[PCR相关] [已完结]PremixTaq(ExTaqVersion2.0 plus dye)与PremixTaq(TaKaRaTaqVersion2.0 plus dye) (评阅+5) (1/6926) 邊緣人灬 2015-01-11 2015-01-12 20:27:19 by 晞朔
[PCR相关] [已完结]土壤DNA提取后做功能基因PCR，试过好多酶都不理想 (2/779) 爱上太阳 2014-10-26 2015-01-12 14:30:03 by aesar9983
[PCR相关] [已完结]什么是假阳性 (3/2836) qaz139687 2015-01-11 2015-01-12 13:04:57 by lzpbar
[PCR相关] [关贴]SEFA-PCR原理 (0/665) 一粒儿糖 2015-01-12 2015-01-12 11:28:42 by 一粒儿糖
[PCR相关] [已完结]求大神帮我看看这电泳图，问题出在哪儿啊 (7/2061) mengzhiyishe 2015-01-08 2015-01-12 09:45:01 by 素锦流年wang
[PCR相关] [已完结]正在扩增李斯特菌的hly基因，但是换了三对引物都扩不出来~ (2/1150) 永远一片天 2015-01-11 2015-01-12 09:10:45 by 18761615793
[PCR相关] [已完结]cDNA降解 (评阅+1) (3/2181) 970402570 2015-01-11 2015-01-11 19:37:08 by 红卫大兵
[PCR相关] [已完结]SSR-PCR (评阅+5) (2/592) liushupeng 2015-01-10 2015-01-10 22:48:57 by zshw_001
[PCR相关] [已完结]左右海马各做PCR、WB可行吗？ (2/874) weiss814 2014-04-10 2015-01-10 18:52:24 by weiss814
[PCR相关] [已完结]用于FAM通道RT-PCR的探针3‘端要怎么设计 (1/1883) chenyanzhen 2014-06-20 2015-01-10 12:14:06 by Swift1204
[PCR相关] [已完结]相对定量Actin曲线怪异 (评阅+1) (1/498) 寒心季 2014-12-25 2015-01-10 06:22:52 by JNVBNC
[PCR相关] [已完结]qPCR结果异常 (5/1581) eskimo_0_0 2015-01-07 2015-01-09 23:37:41 by xygcdmr
[PCR相关] 关于内参 (0/240) ws8601 2015-01-09 2015-01-09 21:13:11 by ws8601
[PCR相关] [已完结]求问怎样查找比较偏门的菌类的基因相应的cDNA并在此基础上RT-PCR设计引物，多谢 (评阅+1) (6/2127) gumimimi 2015-01-07 2015-01-09 17:57:30 by gumimimi
[PCR相关] [已完结]BLAST使用 (评阅+1) (3/769) jingmi 2015-01-09 2015-01-09 15:12:22 by jingmi
[PCR相关] [已完结]请教未知噬菌体鉴定!!! (评阅+1) (1/1778) swgcpy 2014-11-27 2015-01-09 13:38:25 by 濯蝶雨518785
[PCR相关] dNTP (18/943) shenyunlong 2014-03-07 2015-01-09 12:50:22 by shenyunlong
[PCR相关] [已完结]Taq 酶扩增的片段能连接平末端载体吗 (3/3091) 颖颖贝 2015-01-08 2015-01-09 11:59:33 by jonew
[PCR相关] [已完结]EasyTaq酶能P出条带而用LATaq酶却只有弥散性条带 (评阅+1) (4/1950) xiangqin90 2015-01-07 2015-01-09 11:45:21 by 18761615793
[PCR相关] 关于一段基因内部的部分高度重复的DNA序列问题 (评阅+1) (1/808) cainiao9405 2015-01-08 2015-01-09 00:39:42 by titus0805
[PCR相关] [已完结]PCR 熔解曲线问题 (1/935) linxuan8889 2015-01-07 2015-01-08 21:56:31 by linxuan8889
[PCR相关] [已完结]多重PCR的酶 (7/4403) 凝霜枫叶 2015-01-04 2015-01-08 20:09:44 by 鳯凰小白
[PCR相关] [已完结]测序结果图 (评阅+1) (2/530) yghboy168 2014-12-14 2015-01-08 17:41:57 by 翼在天
[PCR相关] [已完结]PCR操作中的问题 (评阅+1) (7/1335) 王雪松_ 2015-01-04 2015-01-08 10:17:00 by 肚糠稀同学
[PCR相关] [已完结]质粒环P (2/5625) why025 2015-01-05 2015-01-07 16:44:43 by why025
[PCR相关] RT-PCR引物设计 (评阅+1) (0/316) shizhi1989 2015-01-07 2015-01-07 16:29:57 by shizhi1989
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