| ²é¿´: 3245 | »Ø¸´: 28 | ||
ÏÄľ³³Ð³æ (³õÈëÎÄ̳)
|
[ÇóÖú]
PCRµÄ½á¹ûÖ»ÓÐÒýÎï¶þ¾ÛÌå³öÀ´£¬Ä¿µÄƬ¶ÎûÓС£»»¹ýºÜ¶àÌõ¼þ¡£Çó´óÉñÖ¸µ¼ ÒÑÓÐ9È˲ÎÓë
|
|
|
×öµÄÊǽðÒø»¨ITS2ÐòÁÐ Ìåϵ ×Ü25ul mix 12.5ul ÒýÎï1 1ul ÒýÎï2 1ul Ë«ÕôË® 7.5ul Ä£°å 3ul ![]() ÏÂÃæÊÇÁ½¸öÒýÎïµÄÌõ´ø¡£²»¹ý¿ÉÄÜͼµ¹ÁË |
» ±¾ÌûÒÑ»ñµÃµÄºì»¨£¨×îÐÂ10¶ä£©
» ²ÂÄãϲ»¶
²»ºÏÀíÍÜ¿ÆÑÐʵÑéÖ®¡°ÊóÌÓÈý´ÎÎÒ·¢ÈýƪSCI¡±
ÒѾÓÐ0È˻ظ´
ÄÉÃ×Á£¶È·ÖÎö
ÒѾÓÐ3È˻ظ´
»¯Ñ§¹¤³Ì¼°¹¤Òµ»¯Ñ§ÂÛÎÄÈóÉ«/·ÒëÔõôÊÕ·Ñ?
ÒѾÓÐ205È˻ظ´
ÁÖ¿ÆÔºÁÖ»¯ËùÕÐÊÕ²ÄÁÏÓ뻯¹¤×¨ÒµË¶Ê¿£¬×ø±êÄϾ©
ÒѾÓÐ0È˻ظ´
Áôѧ--²©Ê¿ÕÐÉú
ÒѾÓÐ16È˻ظ´
»¯Ñ§¹¤³Ì£¬±¾211£¬Çóµ÷¼Á£¬abÇø²»ÏÞ
ÒѾÓÐ4È˻ظ´
ºþ±±Ê¦·¶´óѧÕе÷¼ÁÀ²
ÒѾÓÐ6È˻ظ´
ÉÛÑôѧԺʳƷÓ뻯ѧ¹¤³ÌѧԺ˶ʿµ÷¼Á
ÒѾÓÐ0È˻ظ´
Ìì½òÉÌÒµ´óѧ ÉúÎïÓëÒ½Ò©¿ÎÌâ×éÕÐÉú
ÒѾÓÐ0È˻ظ´
ÉúÎﻯ¹¤Ñ§Ë¶ÕÐÊÕµ÷¼Á
ÒѾÓÐ0È˻ظ´
» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:
¼±¼±¼±£¡£¡£¡¹ØÓÚpcrÒýÎïÉè¼Æ
ÒѾÓÐ14È˻ظ´
Ò»ÖÖDNAÒ»ÖÖÒýÎïPCRÖ®ºóÊDz»ÊÇÖ»ÓÃÒ»¸öÌõ´ø£¿
ÒѾÓÐ7È˻ظ´
PCRÀ©ÔöûÓнá¹û£¬Çó½ÌÊÇÒýÎïTmÖµÏà²îÌ«´ó£¬»¹ÊÇÄ£°åGCº¬Á¿Ì«¸ß£¿
ÒѾÓÐ15È˻ظ´
ÓÃÆ¬¶ÎÉϵÄÒýÎï¾úÒºPCR£¬£¬³öÀ´Ìõ´øµÄ´óС²»¶Ô~~
ÒѾÓÐ2È˻ظ´
¹¹½¨ÔØÌåPCR½á¹ûºÍ²âÐò½á¹û²»Ò»Ñù
ÒѾÓÐ4È˻ظ´
PCR½á¹ûÖ»ÓÐÒýÎï¶þ¾ÛÌåµÄÌõ´ø£¬Ã»ÓвúÎï
ÒѾÓÐ6È˻ظ´
µ¥ÒýÎïÀ©Ôö
ÒѾÓÐ5È˻ظ´
¾úÂäPCR
ÒѾÓÐ11È˻ظ´
ÓйØPCRɸÒýÎïµÄÎÊÌâ~~
ÒѾÓÐ3È˻ظ´
СĿµÄƬ¶ÎµÄ²âÐò¼°Éè¼ÆÒýÎïµÄÒÉÎÊ
ÒѾÓÐ2È˻ظ´
ÖØµþPCRÒýÎÁ½¸öƬ¶Î·Ö±ð»ØÊÕºó´æÔÚÔÓ´øÊÇÔõô»ØÊÂ
ÒѾÓÐ4È˻ظ´
ÖØÐºϳɵÄÄÚ²ÎÒýÎïÓÐÒýÎï¶þ¾ÛÌå¸ÃÕ¦°ì£¿
ÒѾÓÐ2È˻ظ´
RT¡ªPCRÒýÎï¡¢Ìõ´øÎÊÌ⣬ÐÂÊÖÇóÖú£¬ÇóÒâ¼û£¬Çó½¨Ò飡
ÒѾÓÐ9È˻ظ´
»ùÒò×éPCR ÎÞ½á¹û
ÒѾÓÐ4È˻ظ´
RT-PCR½á¹û·ÖÎöÇóÖú
ÒѾÓÐ5È˻ظ´
¾úÒºPCR½á¹û²»ÕýÈ·
ÒѾÓÐ4È˻ظ´
PCRºó²âÐò£¬blast×î¸ßÆ¥Åä¶ÈÖ»ÓÐ32%
ÒѾÓÐ3È˻ظ´
PCRûÓнá¹û
ÒѾÓÐ5È˻ظ´
¡¾ÇóÖú/½»Á÷¡¿ÒýÎïÌØÒìÐÔPCR£¨SSP-PCR£©¼ì²âSNP£¬ÊDz»ÊÇÈÝÒ׳öÏÖÔÓ´øÑ½
ÒѾÓÐ11È˻ظ´
¡¾ÇóÖú/½»Á÷¡¿pcrÎÞ½á¹û
ÒѾÓÐ8È˻ظ´
¡¾ÇóÖú/½»Á÷¡¿PCRÒýÎï¶þ¾ÛÌåÎÊÌâ
ÒѾÓÐ11È˻ظ´
¡¾ÇóÖú/½»Á÷¡¿ÒýÎïTmÖµºÜ¸ß£¬PCRÇóÖú
ÒѾÓÐ17È˻ظ´
¡¾·ÖÏí¡¿realtime pcr ¸öÈ˾Ñé·ÖÏí
ÒѾÓÐ41È˻ظ´
·´Ó¦Á´
гæ (ÕýʽдÊÖ)
- Ó¦Öú: 6 (Ó×¶ùÔ°)
- ½ð±Ò: 397.3
- É¢½ð: 339
- ºì»¨: 16
- Ìû×Ó: 358
- ÔÚÏß: 183.1Сʱ
- ³æºÅ: 3334128
- ×¢²á: 2014-07-22
- ÐÔ±ð: GG
- רҵ: ÃâÒßѧ
¡ï
Î÷ÃÅ´µÑ©170: ½ð±Ò+1, ¹ÄÀø»ØÌû½»Á÷ 2016-01-13 22:51:07
Î÷ÃÅ´µÑ©170: ½ð±Ò+1, ¹ÄÀø»ØÌû½»Á÷ 2016-01-13 22:51:07
|
I think you should use a positive control, such as amplification of housekeeping gene (Actin, etc.), which really does much help for troubleshooting. Therefore, you should amplify a housekeeping gene using your DNA samples tomorrow. |
5Â¥2016-01-13 20:02:14
ÏÄľ³³
гæ (³õÈëÎÄ̳)
- Ó¦Öú: 1 (Ó×¶ùÔ°)
- ½ð±Ò: 139.4
- ºì»¨: 1
- Ìû×Ó: 26
- ÔÚÏß: 10.2Сʱ
- ³æºÅ: 3735011
- ×¢²á: 2015-03-13
- רҵ: Ö²ÎïÉúÀíÓëÉú»¯
|
³ýÁË58cÍË»ðÒÔÍâ¡£56 59 60 ¶¼×öÁË¡£Ð§¹û²î²»¶à¡£ Ìåϵģ°å2ul 3ul 4ul 5ul ¶¼×öÁË¡£½á¹ûûÓÐʲô±ä»¯ |
3Â¥2016-01-13 19:45:34
chiemo
гæ (³õÈëÎÄ̳)
- Ó¦Öú: 1 (Ó×¶ùÔ°)
- ½ð±Ò: 13
- Ìû×Ó: 2
- ÔÚÏß: 2.3Сʱ
- ³æºÅ: 3947257
- ×¢²á: 2015-06-30
- רҵ: ʳƷ¿ÆÑ§»ù´¡
¡¾´ð°¸¡¿Ó¦Öú»ØÌû
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
| ÄãÔõôÅж¨ÊÇÒýÎï¶þ¾ÛÌåµÄ£¿ÊÇ·ñÒòΪºÏ³ÉµÄÒýÎï²»´¿£¿¿ÉÒÔÊÔÊÔtouchdown PCR£¬ÎÒ֮ǰÅܳöÀ´ÓÐÔÓ´ø£¬touchdownºó¾ÍÖ»ÓÐÄ¿±êÐòÁÐÁË |
6Â¥2016-01-14 10:48:41
Ciruela
Ìú³æ (³õÈëÎÄ̳)
- Ó¦Öú: 4 (Ó×¶ùÔ°)
- ½ð±Ò: 42
- Ìû×Ó: 6
- ÔÚÏß: 5.5Сʱ
- ³æºÅ: 4340178
- ×¢²á: 2016-01-07
- ÐÔ±ð: MM
7Â¥2016-01-14 13:25:34
ÄäÃû
Óû§×¢Ïú (СÓÐÃûÆø)
- Ó¦Öú: 0 (Ó×¶ùÔ°)
- ½ð±Ò: 275.1
- ºì»¨: 1
- Ìû×Ó: 84
- ÔÚÏß: 9.5Сʱ
- ³æºÅ: 0
- ×¢²á: 2015-12-13
- ÐÔ±ð: GG
- רҵ: ÉúÎﻯѧ
8Â¥2016-01-14 13:35:14
·Ö×Ó2010
Òø³æ (СÓÐÃûÆø)
- Ó¦Öú: 3 (Ó×¶ùÔ°)
- ½ð±Ò: 423.2
- É¢½ð: 35
- ºì»¨: 2
- Ìû×Ó: 67
- ÔÚÏß: 30.2Сʱ
- ³æºÅ: 2341854
- ×¢²á: 2013-03-12
- רҵ: Ö²ÎïÉúÀíÓëÉú»¯
¡¾´ð°¸¡¿Ó¦Öú»ØÌû
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
| ÓÃactin¼ì²âÄ£°å£¬¿´Ä£°åÊDz»ÊÇÓÐÎÊÌâ¡£Èç¹ûûÓÐÎÊÌâ¾Í½µµÍÄ£°åµÄÁ¿£¬1-2ul£¬56¶ÈÔÚÊÔÊÔ¡£Èç¹û»¹ÊDz»ÐоÍÊÇÒýÎïµÄÎÊÌâ |

10Â¥2016-01-14 14:41:36
ss2032
ľ³æ (ÕýʽдÊÖ)
ÏÌÓã
- Ó¦Öú: 7 (Ó×¶ùÔ°)
- ½ð±Ò: 3166.3
- ºì»¨: 3
- Ìû×Ó: 577
- ÔÚÏß: 70.8Сʱ
- ³æºÅ: 3590774
- ×¢²á: 2014-12-12
- ÐÔ±ð: MM
- רҵ: ϸ°ûÔöÖ³¡¢Éú³¤Óë·Ö»¯

11Â¥2016-01-14 14:52:09
ÏÄľ³³
гæ (³õÈëÎÄ̳)
- Ó¦Öú: 1 (Ó×¶ùÔ°)
- ½ð±Ò: 139.4
- ºì»¨: 1
- Ìû×Ó: 26
- ÔÚÏß: 10.2Сʱ
- ³æºÅ: 3735011
- ×¢²á: 2015-03-13
- רҵ: Ö²ÎïÉúÀíÓëÉú»¯
2Â¥2016-01-13 19:42:41
½ðľÑÐôôßÕ
гæ (СÓÐÃûÆø)
- Ó¦Öú: 1 (Ó×¶ùÔ°)
- ½ð±Ò: 187.1
- É¢½ð: 65
- ºì»¨: 8
- Ìû×Ó: 162
- ÔÚÏß: 13.2Сʱ
- ³æºÅ: 4354794
- ×¢²á: 2016-01-13
- רҵ: Ë®²úÑøÖ³Ñ§
¡¾´ð°¸¡¿Ó¦Öú»ØÌû
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
|
ûÓÐÌõ´øµÄ»°¾Í½µÎ£¬ÊÔÊÔ56£¬55£¬54£¬53£¬52 ·¢×ÔСľ³æAndroid¿Í»§¶Ë |
4Â¥2016-01-13 19:51:12
±ßľéä
ľ³æ (СÓÐÃûÆø)
- Ó¦Öú: 7 (Ó×¶ùÔ°)
- ½ð±Ò: 2802.2
- É¢½ð: 10
- ºì»¨: 4
- Ìû×Ó: 126
- ÔÚÏß: 109.7Сʱ
- ³æºÅ: 2659652
- ×¢²á: 2013-09-16
- ÐÔ±ð: GG
- רҵ: ¿¹Ì幤³Ìѧ
9Â¥2016-01-14 14:17:18
















»Ø¸´´ËÂ¥
ÏÄľ³³
