15楼: Originally posted by pengc201 at 2016-01-17 01:03:56
这个有太多的可能了。。
1. 模版浓度太低或者质量不好。(do a housekeeping gene positive control)
2. annealing temperature is not optimized, do a gradient PCR
3. are you sure the extension time is en ...

10?: Originally posted by ????2010 at 2016-01-14 14:41:36
??actin?????壬??????????????????????????????????????1-2ul??56??????????????????о????????????

4?: Originally posted by ????????? at 2016-01-13 19:51:12
????????????????????56??55??54??53??52

22?: Originally posted by ????? at 2016-01-21 12:02:30
总之。多谢建议，我会好好加油的...

23?: Originally posted by ????? at 2016-01-21 12:06:56
??过了，没有什么用，???能也有其他方???问题。我??看看...

11楼: Originally posted by ss2032 at 2016-01-14 14:52:09
一般不是做30个循环么？？？...

6楼: Originally posted by chiemo at 2016-01-14 10:48:41
你怎么判定是引物二聚体的？是否因为合成的引物不纯？可以试试touchdown PCR，我之前跑出来有杂带，touchdown后就只有目标序列了