Detoxification of gold nanorods by treatment with polystyrenesulfonate
Authors: Alexei P Leonov, Jiwen Zheng, Jeffrey D Clogston, Stephan T Stern, Anil K Patri, Alexander Wei
Journal: ACS Nano
We address an outstanding issue associated with the biocompatibility of gold nanorods (GNRs), a promising agent for biomedical imaging and theragnostics. GNRs are typically prepared in the presence of cetyltrimethylammonium bromide (CTAB), a cationic surfactant whose rigorous removal is necessary due to its cytotoxicity and membrane-compromising properties. CTAB-stabilized GNRs can be partially purified by treatment with polystyrenesulfonate (PSS), an anionic polyelectrolyte often used as a surrogate peptizing agent, followed by chloroform extraction and ultrafiltration with minimal loss of dispersion stability. However, in vitro cytotoxicity assays of PSS-coated GNRs revealed IC(50) values in the low to submicromolar range, with subsequent studies indicating the source of toxicity to be associated with a persistent PSS-CTAB complex. Further exchange of CTAB-laden PSS with fresh polyelectrolyte greatly improves biocompatibility, to the extent that 85 microg/mL of "CTAB-free" GNRs (the highest level evaluated) has comparable toxicity to a standard phosphate buffer solution. Ironically, PSS is not effective by itself at stabilizing GNRs in CTAB-depleted suspensions: while useful as a detergent for GNR detoxification, it should be replaced by more robust coatings for long-term stability under physiological conditions ,
CTAB毒性很大,能不用就不用
CAS:57-09-0
分子式:C19H42BrN
分子质量:364.45
熔点:250-237℃
中文名称:十六烷基三甲基溴化铵
N,N,N-三甲基-1-十六烷基溴化铵
鲸熔三甲基溴化铵
阳性皂
英文名称:Hexadecyltrimethyl ammonium bromide
Cetyltrimethylammonium bromide
hexadecyltrimethyl-ammoniu bromide
(1-hexadecyl)trimethylammonium bromide
n,n,n-trimethyl-1-hexadecanaminiu bromide
acetoquat ctab
性状描述:白色微晶形粉末。熔点>230℃(分解)。溶于水,微溶于醇,不溶于醚。水溶液呈碱性,产生大量气泡,具刺鼻臭味。
生产方法:由十六醇经溴素溴化后与三甲胺反应生成季铵盐。
用途:用于有机合成,也可作为阳离子表面活性剂,用作杀菌剂,配制含漱剂、消毒剂,肠内葡萄糖吸收抑制剂。该品也是常用的胶束增溶分光光度法试剂。
楼上的瞎侃,怎么会很大毒呢?!
同意三楼,CTAB是很常用的表面活性剂,而且效果很不错
2楼在不懂装懂吧?
我经常用
各位做一下mtt就知道了,
你没做过毒性吧
Detoxification of gold nanorods by treatment with polystyrenesulfonate
Authors: Alexei P Leonov, Jiwen Zheng, Jeffrey D Clogston, Stephan T Stern, Anil K Patri, Alexander Wei
Journal: ACS Nano
We address an outstanding issue associated with the biocompatibility of gold nanorods (GNRs), a promising agent for biomedical imaging and theragnostics. GNRs are typically prepared in the presence of cetyltrimethylammonium bromide (CTAB), a cationic surfactant whose rigorous removal is necessary due to its cytotoxicity and membrane-compromising properties. CTAB-stabilized GNRs can be partially purified by treatment with polystyrenesulfonate (PSS), an anionic polyelectrolyte often used as a surrogate peptizing agent, followed by chloroform extraction and ultrafiltration with minimal loss of dispersion stability. However, in vitro cytotoxicity assays of PSS-coated GNRs revealed IC(50) values in the low to submicromolar range, with subsequent studies indicating the source of toxicity to be associated with a persistent PSS-CTAB complex. Further exchange of CTAB-laden PSS with fresh polyelectrolyte greatly improves biocompatibility, to the extent that 85 microg/mL of "CTAB-free" GNRs (the highest level evaluated) has comparable toxicity to a standard phosphate buffer solution. Ironically, PSS is not effective by itself at stabilizing GNRs in CTAB-depleted suspensions: while useful as a detergent for GNR detoxification, it should be replaced by more robust coatings for long-term stability under physiological conditions
,