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wizardfan: ½ð±Ò+2, ¸ÐлӦÖú 2014-12-18 10:47:30
I am trying to comment on your problem from a amateur's point of view.
the molecular weight markers are indicating the molecular weight in a proximate manner, not at all accurate, nor are they linear.
the markers from different companies behave differently. For me, fermentas and bio-rad markers are better. but you cannot expect very accurate MW indication. Usually, we are trying to get positive control loading to confirm that the band you see is what you want.
good luck.
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one more comment:
if you really want to predict the molecular weight in this way, you should do with a molecular weight marker with more broader spectrum. that is to say, the largest molecular weight indicator should be over 280kDa and you can still see the linear regression. In your case, the largest marker is 250kDa and your target band is out of the range. You cannot do linear regression outside your standard since you never know if that part is still linear.
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3Â¥2014-12-17 07:13:35
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