Znn3bq.jpeg
²é¿´: 2469  |  »Ø¸´: 11
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

duke188

гæ (³õÈëÎÄ̳)

[ÇóÖú] ÌݶÈPCRÍË»ðζÈ

ÉÏÓÎTTACAAGATGGAGCCTCACC(GCº¬Á¿50%)
Tm(1M Na+)£º68.2
Tm(50mM Na+)£º46.6
ÏÂÓÎCAAAGAATAACCCAGGACGA(GCº¬Á¿45%)
Tm(1M Na+)£º66.2
Tm(50mM Na+)£º44.6
˳±ãÎÊÏ´ó¼Ò£¬Tm(1M Na+)ºÍTm(50mM Na+)ÓÐÊ²Ã´Çø±ð°¡?
ÎÒ¸ÃÑ¡ÔñÄĸöTm°¡£¿
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

cicelyzh

Ìú¸Ëľ³æ (Ö°Òµ×÷¼Ò)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
kx444555: ½ð±Ò+1, ¹ÄÀø½»Á÷ 2013-07-22 11:49:07
ÒýÓûØÌû:
3Â¥: Originally posted by duke188 at 2013-07-21 22:20:52
ÎÒÊÇÐÂÊÖ£¬ÓÉÓÚÒª×öÌݶÈPCRÃþÍË»ðζȣ¬°´ÕÕTm(50mM Na+)--5¡æµÄ»°£¬ÍË»ðζÈÊDz»ÊÇÌ«µÍÁË...

Ò»°ãPCRʱµÄÍË»ðζȺÜÉÙÑ¡ÔÚ50¡ãCÒÔÏ¡£´ÓÄãµÄÒýÎï´ó¸ÅÊÇ20nt£¬GCº¬Á¿ÔÚ50%×óÓÒ£¬ÍË»ðζÈÒ»°ãÔÚ55-60¡ãC¡£Äã¿ÉÒÔ¸ù¾ÝÕâ¸öÉè¼ÆÄãµÄζÈÌݶȡ£
4Â¥2013-07-22 04:27:58
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 12 ¸ö»Ø´ð

shangrila911

гæ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
duke188(gyesang´ú·¢): ½ð±Ò+2, ¹ÄÀø»ØÌûÓ¦Öú£¡ 2013-07-21 23:05:54
Tm(1M Na+)ºÍTm(50mM Na+)  À¨ºÅÀïÃæµÄ±íʾÑÎŨ¶È£¬ÑÎŨ¶ÈÔ½¸ß£¬TmÔ½¸ß£¬²»¹ý»¹ÊǸù¾Ý×Ô¼ºµÄʵ¼ÊPCRÇé¿ö¶ø¶¨£¬Ò»°ãPCR³£ÓÃTm(50mM Na+)×÷²Î¿¼...
Ò»Çж¼»áºÃµÄ!
2Â¥2013-07-21 22:01:11
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

duke188

гæ (³õÈëÎÄ̳)

ÒýÓûØÌû:
2Â¥: Originally posted by shangrila911 at 2013-07-21 22:01:11
Tm(1M Na+)ºÍTm(50mM Na+)  À¨ºÅÀïÃæµÄ±íʾÑÎŨ¶È£¬ÑÎŨ¶ÈÔ½¸ß£¬TmÔ½¸ß£¬²»¹ý»¹ÊǸù¾Ý×Ô¼ºµÄʵ¼ÊPCRÇé¿ö¶ø¶¨£¬Ò»°ãPCR³£ÓÃTm(50mM Na+)×÷²Î¿¼...

ÎÒÊÇÐÂÊÖ£¬ÓÉÓÚÒª×öÌݶÈPCRÃþÍË»ðζȣ¬°´ÕÕTm(50mM Na+)--5¡æµÄ»°£¬ÍË»ðζÈÊDz»ÊÇÌ«µÍÁË
3Â¥2013-07-21 22:20:52
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

Sthunder

ľ³æ (ÕýʽдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
kx444555: ½ð±Ò+1, ¹ÄÀø½»Á÷ 2013-07-22 11:49:12
¸ù¾ÝÄãµÄÐòÁУ¬¸Ð¾õÄãÕýÏòÒýÎï3¶ËGCº¬Á¿Óеã¸ß£¬²»¹ýÓ¦¸ÃûÓжà´ó¹ØÏµ£¬Ö±½Ó×ö¸ö50£¬ 48,46µÄTD pcr¾ÍûÎÊÌ⣡Good luck£¡
»¥Öú»¥Àø£¬¹²·Ü¹²½ø£¡£¡
5Â¥2013-07-22 05:31:19
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] »¯Ñ§¹¤³Ìµ÷¼Á289 +41 yangæÃ 2026-04-07 47/2350 2026-04-09 23:23 by wolf97
[¿¼ÑÐ] 297Çóµ÷¼Á +27 GENJIOW 2026-04-07 30/1500 2026-04-09 23:20 by wolf97
[¿¼ÑÐ] ±¾¿Æ211 ¹¤¿Æ085400 280·ÖÇóµ÷¼Á ¿É¿çרҵ +3 LZH£¨µÈ´ýµ÷¼ÁÖÐ 2026-04-09 3/150 2026-04-09 21:29 by wutongshun
[¿¼ÑÐ] 283Çóµ÷¼Á£¬¹¤¿Æ£¡ +8 ËÕ´òË®7777 2026-04-08 8/400 2026-04-09 20:50 by xujun0624
[¿¼ÑÐ] µç×ÓÐÅÏ¢270Çóµ÷¼Á +11 terminal469 2026-04-07 11/550 2026-04-09 18:28 by hy861222
[¿¼ÑÐ] 296Çóµ÷¼Á +5 Íô£¡£¿£¡ 2026-04-09 5/250 2026-04-09 17:47 by ÄûÃʲ»Ëázy
[¿¼ÑÐ] 085404£¬334·Ö£¬Çóµ÷¼Á +5 sunjie8888 2026-04-08 8/400 2026-04-09 07:26 by sunjie8888
[¿¼ÑÐ] ²ÄÁÏ¿¼ÑÐÇóµ÷¼Á×Ü·Ö280 +30 mkjlz1 2026-04-06 35/1750 2026-04-08 21:25 by cyh¡ª315
[¿¼ÑÐ] 270Çóµ÷¼Á +3 031127 2026-04-06 4/200 2026-04-08 21:00 by ÄæË®³Ë·ç
[¿¼ÑÐ] ÉúÎïѧ308·ÖÇóµ÷¼Á£¨Ò»Ö¾Ô¸»ª¶«Ê¦´ó£©×ö¹ý·Ö×ÓʵÑé +6 ÏàÐűػá¹ââÍòÕ 2026-04-07 7/350 2026-04-08 16:49 by tjzhao
[¿¼ÑÐ] 0854µç×ÓÐÅÏ¢319Çóµ÷¼Á£¨½ÓÊÜ¿çרҵµ÷¼Á£© +5 ÐÇÐDz»Õ£ÑÛà¶ 2026-04-05 6/300 2026-04-07 22:16 by hemengdong
[¿¼ÑÐ] ÐŹ¤Ëù11408 340·Ö ±¾¿ÆÎ÷°²½»´ó×Ô¶¯»¯ +3 moontrek 2026-04-06 3/150 2026-04-07 09:56 by chongya
[¿¼ÑÐ] 292Çóµ÷¼Á +4 lilllllxccc 2026-04-05 5/250 2026-04-07 09:29 by ·Ä´óÑîÀÏʦ
[¿¼ÑÐ] Çóµ÷¼Á +4 wos666 2026-04-03 5/250 2026-04-06 15:22 by wos666
[¿¼ÑÐ] ²ÄÁϵ÷¼Á +13 Ò»ÑùYWY 2026-04-03 14/700 2026-04-05 18:20 by À¶ÔÆË¼Óê
[¿¼ÑÐ] ¹¤¿Æ277·ÖÇóµ÷¼Á²ÄÁÏ +8 ÉÏÁËÉÏÁËÉÏŶ 2026-04-05 9/450 2026-04-05 13:05 by wwytracy
[¿¼ÑÐ] µ÷¼Á +8 ÐܶþÏëÉϰ¶ 2026-04-04 8/400 2026-04-05 05:27 by houyaoxu
[¿¼ÑÐ] 292Çóµ÷¼Á +11 2022080213 2026-04-04 13/650 2026-04-04 18:38 by macy2011
[¿¼ÑÐ] 295Çóµ÷¼Á +3 ÉÐټѽ 2026-04-03 4/200 2026-04-03 21:23 by zhq0425
[¿¼ÑÐ] 081200-11408-276ѧ˶Çóµ÷¼Á +5 ´Þwj 2026-04-03 5/250 2026-04-03 15:06 by arrow8852
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û