Publisher:  Humana Press
Number Of Pages:  336
Publication Date:  2005-01-19
ISBN / ASIN:  1588292533


Book Description:

Leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for freeduan.com human health, nutrition, and environmental protection. Overview chapters on microorganisms as a source of metabolic and enzymatic diversity, and on the fast-moving field of enzyme biosensors are presented. Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.

Contents
Preface ..............................................................................................................v
Contributors ..................................................................................................... ix
1 Microbial Cells and Enzymes: A Century of Progress
Jos¨¦ L. Adrio and Arnold L. Demain ..................................................... 1
2 Enzyme Biosensors
Steven J. Setford and Jeffrey D. Newman .......................................... 29
3 Directed Evolution of Enzymes: How to Improve Nucleic Acid
Polymerases for Biocatalysis
Susanne Brakmann and Sascha Nico Stumpp ..................................... 61
4 L-Glutaminase as a Therapeutic Enzyme of Microbial Origin
Abdulhameed Sabu, K. Madhavan Nampoothiri, and Ashok Pandey ..... 75
5 Enzymatic Production of D-Amino Acids
Hemraj S. Nandanwar, Gurinder S. Hoondal, and Rakesh M. Vohra .... 91
6 Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides
Oskar Ba?uelos, Maria del Carmen Ronchel, Jos¨¦ Luis Adrio, and Javier Velasco ................ 105
7 Food-Grade Corynebacteria for Enzyme Production
Jos¨¦ A. Gil, Angelina Ramos, Sirin A. I. Adham, Noelia Valbuena, Michal Letek, and Luis M. Mateos ................... 115
8 Enzymatic Synthesis of (S)-Phenylalanine and Related (S)-Amino Acids by Phenylalanine Dehydrogenase
Yasuhisa Asano ................................................................................. 141
9 Enzymes in Modern Detergents
Susumu Ito, Tohru Kobayashi, Yuji Hatada, and Koki Horikoshi ..... 151
10 Microbial Proteases
Chandran Sandhya, K. Madhavan Nampoothiri, and Ashok Pandey ... 165
11 Proteases Produced by Halophilic Bacteria and Archaea
Encarnaci¨®n Mellado, Cristina S¨¢nchez-Porro, and Antonio Ventosa .... 181
12 Microbial Pectinases
Rupinder Tewari, Ram P. Tewari, and Gurinder S. Hoondal ........... 191
13 Expression of Microbial Phytases in Yeast Systems and Characterization of the Recombinant Enzymes
Xin Gen Lei and Taewan Kim ........................................................... 209
14 Glucose Dehydrogenase for the Regeneration of NADPH and NADH
Andrea Weckbecker and Werner Hummel ...................................... 225
15 Acetate Kinase from Methanosarcina thermophila, a Key Enzyme
for Methanogenesis
Prabha Iyer and James G. Ferry ........................................................ 239
16 Immobilization of Enzymes by Covalent Attachment
Scott J. Novick and J. David Rozzell ................................................ 247
17 Preparation of an Industrial Biocatalyst of Penicillin G Acylase
on Sepabeads: Improving the Design of Penicillin G Hydrolysis
Cesar Mateo, Valeria Grazu, Olga Abian, Manuel Fuentes,
Gloria Fern¨¢ndez-Lorente, Jos¨¦ M. Palomo,
Roberto Fern¨¢ndez-Lafuente, and Jos¨¦ M. Guis¨¢n....................... 273
18 Methods for Bioencapsulation of Enzymes and Cells
Thomas M. S. Chang ......................................................................... 289
Index ............................................................................................................
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