²é¿´: 4370  |  »Ø¸´: 21

czcpudai

Í­³æ (ÕýʽдÊÖ)

[ÇóÖú] Çë½ÌPCR¸ßÊÖ£¬Ê¹ÓÃSYBR Green I·¨½øÐÐÓ«¹â¶¨Á¿PCR¶ÔÓÚ²úÎï´óСÓÐʲôҪÇó£¿ ÒÑÓÐ3È˲ÎÓë

±¾ÈË´òËã×öÓ«¹â¶¨Á¿PCR£¬ÏÖÔÚ×öÁËÆÕͨµÄrt-pcr£¬ÈýÖÖ»ùÒòµÄ´óСΪ352bp£¬362bp£¬228bp£¬½á¹û¶¼À©Ôö³öÀ´ÁË£¬ÏÂÒ»²½´òËã×öÓ«¹â¶¨Á¿RT-PCR£¬Ó«¹âȾÁÏΪSYBR£¬ÇëÎÊÊDz»ÊÇ¿ÉÒÔ½Ó×ÅʹÓÃÆÕͨRt-PCRÖÐËùʹÓõÄÒýÎïÄØ£¬»¹ÊÇÖØÐÂÉè¼ÆÒýÎʹÆä²úÎï´óСÔÚ50-150bpÖ®¼ä£¿£¿£¿Çó½â´ð£¿£¿
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

mutou1025

Í­³æ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
Сµ¤Ä¾Ä¾: ½ð±Ò+1, ¹ÄÀø½»Á÷ 2012-08-22 15:16:25
Ò»°ãÔÚ100bp×óÓÒ£¬ÖØÐÂÉè¼ÆÒýÎï¡£ÄãµÄ300¶àbpÌ«´óÁË¡£SYBRÎҼǵóÌÐò±äÐÎÍË»ðʱ¼ä ºÃÏñ±ÈÆÕͨPCRʱ¼ä¶Ì¡£Ì«´óÁ˻᲻»áûÓÐÍêÈ«P³öÀ´°¡
2Â¥2012-08-22 13:25:33
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ywding0628

гæ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
Сµ¤Ä¾Ä¾: ½ð±Ò+1, ¹ÄÀø½»Á÷ 2012-08-22 15:16:34
Ò»°ã¶¼ÔÚ80-150bpÖ®¼ä£¬½¨ÒéÖØÐÂÉè¼ÆÒýÎï¡£Èç¹ûÊÇSOSO FAST£¨BIORAD£©µÄmix£¬ ÍË»ðÑÓÉìÒ»¹²Ö»ÓÐ5s¡£
ÐÔ¸ñÂñÏÂÁËÈËÉúµÄ·ü±Ê~
3Â¥2012-08-22 14:31:18
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

chenguestc

ľ³æ (Ö°Òµ×÷¼Ò)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
Сµ¤Ä¾Ä¾: ½ð±Ò+2, ¹ÄÀøÓ¦Öú£¬ÆßϦ¿ìÀÖ 2012-08-23 15:55:31
228¿ÉÒÔÊÔÊÔ¡£
ÎÒ×ö¹ý80-400bpµÄ£¬200¶àµãÓеÄÒýÎﻹÊÇ¿ÉÒԵ쬵«ÊÇÆ¬¶ÎÌ«³¤PCRЧÂʾÍÓеãµÍ£¬²¢ÇÒÓзÇÌØÒìPEAK, ÎÒÒ»°ãÓÃ60¶ÈÍ˻𣬵«ÊÇÌá¸ßζȵ½65¶È£¬¿ÉÒÔ¼õÉÙ»òÕßÏû³ý·ÇÌØÒìPEAK.
¾ßÌåÌõ¼þ²»ÄÜÍêÈ«¸ù¾Ý±¨µÀ£¬¾ßÌåÇé¿ö¾ßÌå·ÖÎö£¬²»¹ý¸öÈ˾õµÃ£¬100-200×óÓҵľͺܺá£Èç¹ûÄãµÄ228Ч¹û²»ºÃ£¬¿ÉÒÔÊÔÊÔÖØÐÂÉè¼ÆÒýÎï°ÑÄ¿±êƬ¶Î¶¨ÓÚ150bp×óÓÒ¡£
4Â¥2012-08-22 20:18:01
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

chenguestc

ľ³æ (Ö°Òµ×÷¼Ò)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï
Сµ¤Ä¾Ä¾: ½ð±Ò+2, ¹ÄÀøÓ¦Öú£¬ÆßϦ¿ìÀÖ 2012-08-23 15:55:52
ÎÒÓõÄÌõ¼þÊÇ£¬
     95¶È 1·ÖÖÓ£¬
45 X  95¶È 15Ãë
     65¶È £¨¾ßÌåζÈÐèÒª×Ô¼ºµ÷Õû£¬ÎÒÓõÄÊÇ65,ËûÈË¿ÉÄÜÒ»°ãÓÃ60¶È£© 45Ãë
×îºóÊÇMELT CUVE,
        95¶È   15Ãë
     65¶È   45Ãë
     95¶È   15Ãë
5Â¥2012-08-22 20:20:35
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

czcpudai

Í­³æ (ÕýʽдÊÖ)

ÒýÓûØÌû:
2Â¥: Originally posted by mutou1025 at 2012-08-22 13:25:33
Ò»°ãÔÚ100bp×óÓÒ£¬ÖØÐÂÉè¼ÆÒýÎï¡£ÄãµÄ300¶àbpÌ«´óÁË¡£SYBRÎҼǵóÌÐò±äÐÎÍË»ðʱ¼ä ºÃÏñ±ÈÆÕͨPCRʱ¼ä¶Ì¡£Ì«´óÁ˻᲻»áûÓÐÍêÈ«P³öÀ´°¡

Ŷ лл¹þ£¡
6Â¥2012-08-23 14:29:20
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

czcpudai

Í­³æ (ÕýʽдÊÖ)

ÒýÓûØÌû:
4Â¥: Originally posted by chenguestc at 2012-08-22 20:18:01
228¿ÉÒÔÊÔÊÔ¡£
ÎÒ×ö¹ý80-400bpµÄ£¬200¶àµãÓеÄÒýÎﻹÊÇ¿ÉÒԵ쬵«ÊÇÆ¬¶ÎÌ«³¤PCRЧÂʾÍÓеãµÍ£¬²¢ÇÒÓзÇÌØÒìPEAK, ÎÒÒ»°ãÓÃ60¶ÈÍ˻𣬵«ÊÇÌá¸ßζȵ½65¶È£¬¿ÉÒÔ¼õÉÙ»òÕßÏû³ý·ÇÌØÒìPEAK.
¾ßÌåÌõ¼þ²»ÄÜÍêÈ«¸ù¾Ý±¨µÀ£¬ ...

¶÷£¬·Ç³£¸Ðл£¡ÎÒ¾õµÃÄú˵µÄͦ¶ÔµÄ£¬Ö»ÊÇÒýÎïÒª¿çÍâÏÔ×ÓÉè¼Æ£¬×Ô¼ºÉè¼ÆÁËһϵ«ÊÇûÓÐÕÒµ½ºÏÊʵģ¬ÒªÃ´ÌØÒìÐԵͣ¬ÒªÃ´TmÖµÏà²î³¬¹ý2¶È£¬»¹ÊÇ×ÐϸÔÙÕÒÕÒ°É
7Â¥2012-08-23 14:36:59
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

czcpudai

Í­³æ (ÕýʽдÊÖ)

ÒýÓûØÌû:
5Â¥: Originally posted by chenguestc at 2012-08-22 20:20:35
ÎÒÓõÄÌõ¼þÊÇ£¬
     95¶È 1·ÖÖÓ£¬
45 X  95¶È 15Ãë
     65¶È £¨¾ßÌåζÈÐèÒª×Ô¼ºµ÷Õû£¬ÎÒÓõÄÊÇ65,ËûÈË¿ÉÄÜÒ»°ãÓÃ60¶È£© 45Ãë
×îºóÊÇMELT CUVE,
        95¶È   15Ãë
     65¶È   45Ãë
     95¶È   15Ãë

½è¼øÁË£¡
8Â¥2012-08-23 14:37:27
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

°¢Ý¼

гæ (СÓÐÃûÆø)

±¾ÌûÄÚÈݱ»ÆÁ±Î

9Â¥2012-11-01 12:01:43
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

dayulee

ľ³æ (ÕýʽдÊÖ)

ÓÞľ³æ

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

ÄãÃÇÉè¼ÆÓ«¹âµÄÒýÎïÊÇÓÃʲôÈí¼þÉè¼Æ°¡
ºÃºÃѧϰ£¬ÌìÌìÏòÉÏ
10Â¥2012-12-03 11:34:30
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ czcpudai µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 296Çóµ÷¼Á +3 www_q 2026-03-18 6/300 2026-03-19 22:28 by zhq0425
[¿¼²©] ¶«»ªÀí¹¤´óѧ»¯²Äרҵ26½ì˶ʿ²©Ê¿ÉêÇë +8 zlingli 2026-03-13 8/400 2026-03-19 16:32 by ÇáËɲ»ÉÙËæ
[¿¼ÑÐ] ÄÜÔ´²ÄÁÏ»¯Ñ§¿ÎÌâ×éÕÐÊÕ˶ʿÑо¿Éú8-10Ãû +4 ÍÑÓ±¶ø³ö 2026-03-16 12/600 2026-03-19 16:17 by ÍÑÓ±¶ø³ö
[¿¼ÑÐ] Çóµ÷¼Á +3 Mqqqqqq 2026-03-19 3/150 2026-03-19 14:11 by peike
[¿¼ÑÐ] 346Çóµ÷¼Á[0856] +3 WayneLim327 2026-03-16 6/300 2026-03-19 11:21 by WayneLim327
[¿¼ÑÐ] ²ÄÁϹ¤³Ìר˶µ÷¼Á +5 204818@lcx 2026-03-17 6/300 2026-03-18 22:55 by 204818@lcx
[¿¼ÑÐ] 311Çóµ÷¼Á +4 ¶¬Ê®Èý 2026-03-18 4/200 2026-03-18 21:47 by ¾¡Ë´Ò¢1
[¿¼ÑÐ] 297Çóµ÷¼Á +8 Ï·¾«µ¤µ¤µ¤ 2026-03-17 8/400 2026-03-18 14:30 by laoshidan
[¿¼ÑÐ] 070300»¯Ñ§319Çóµ÷¼Á +6 ½õÀð0909 2026-03-17 6/300 2026-03-18 13:22 by Iveryant
[¿¼ÑÐ] 268Çóµ÷¼Á +6 ¼òµ¥µã0 2026-03-17 6/300 2026-03-18 09:04 by Î޼ʵIJÝÔ­
[¿¼ÑÐ] 293Çóµ÷¼Á +11 zjlµÄºÅ 2026-03-16 16/800 2026-03-18 08:10 by zhukairuo
[¿¼²©] 26É격 +4 °Ë6°Ë68 2026-03-16 4/200 2026-03-17 13:00 by ÇáËɲ»ÉÙËæ
[¿¼ÑÐ] [µ¼Ê¦ÍƼö]Î÷ÄϿƼ¼´óѧ¹ú·À/²ÄÁϵ¼Ê¦ÍƼö +3 ¼â½ÇСºÉ 2026-03-16 6/300 2026-03-16 23:21 by ¼â½ÇСºÉ
[¿¼ÑÐ] 318Çóµ÷¼Á +3 Yanyali 2026-03-15 3/150 2026-03-16 16:41 by houyaoxu
[¿¼ÑÐ] 277²ÄÁÏ¿ÆÑ§Ó빤³Ì080500Çóµ÷¼Á +3 ×ÔÓɼå±ý¹û×Ó 2026-03-16 3/150 2026-03-16 14:10 by ÔËÆøyunqi
[¿¼ÑÐ] 0703 ÎïÀí»¯Ñ§µ÷¼Á +3 ÎÒ¿ÉÒÔÉϰ¶µÄ¶Ô 2026-03-13 5/250 2026-03-16 10:50 by ÎÒ¿ÉÒÔÉϰ¶µÄ¶ÔÂ
[¿¼ÑÐ] 327Çóµ÷¼Á +6 ʰ¹âÈÎȾ 2026-03-15 11/550 2026-03-15 22:47 by ʰ¹âÈÎȾ
[¿¼ÑÐ] 22408×Ü·Ö284Çóµ÷¼Á +3 InAspic 2026-03-13 3/150 2026-03-15 11:10 by zhq0425
[¿¼ÑÐ] ÕÐÊÕ0805£¨²ÄÁÏ£©µ÷¼Á +3 18595523086 2026-03-13 3/150 2026-03-14 00:33 by 123%¡¢
[¿¼ÑÐ] 070303Ò»Ö¾Ô¸Î÷±±´óѧѧ˶310ÕÒµ÷¼Á +3 dÈçÔ¸Éϰ¶ 2026-03-13 3/150 2026-03-13 10:43 by houyaoxu
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û