ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%,
一般肿瘤细胞多用1640
一般悬浮的细胞培养用1640;贴壁的一般用DMEM,建议楼主在ATCC中看下它们推荐使用的
我们实验室用DMEM高糖培养基养, 貌似都行
DMEM,我们一直用这个培养的,没什么问题
两种都行。随便用!
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%,
一直用DMEM+10%FBS, 细胞养得挺好的