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5Â¥: Originally posted by ÕÂÐÅÀ´ at 2013-04-03 19:27:12
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robustli

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How do you optimize your PCR condition? I have met the same thing. I use KOD to amplify my target gene. I only got the small fragment. Later I increased the denature and primer annealing time (from 20s to 25s). Interestingly, the bigger band appeared.
immunologyparasite
3Â¥2013-04-02 21:11:06
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3Â¥: Originally posted by robustli at 2013-04-02 21:11:06
How do you optimize your PCR condition? I have met the same thing. I use KOD to amplify my target gene. I only got the small fragment. Later I increased the denature and primer annealing time (from 2 ...

in fact, i haven't  solve this question yet, i want to do northern-blot, your suggestion maybe work, i will try it, thanks!
the big problem of AS is how to study the function, especially the different function to wild type gene, do you have any good idea?
4Â¥2013-04-03 19:19:38
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