±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 9811  |  »Ø¸´: 25
±¾Ìû²úÉú 2 ¸ö MolEPI £¬µã»÷ÕâÀï½øÐв鿴
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

[½»Á÷] ·¢ÏÖÁËbeta actin×÷ΪÄڲεÄÒ»¸ö©¶´

ÖÚËùÖÜÖª£¬¦Â-actin×÷Ϊ»ùÒò¶¨Á¿»ò¶¨ÐÔµÄÄڲΣ¬ÔÚPCRʵÑéÖÐÓÐ׏㷺ӦÓᣵ«ÊÇС³æÔÚ¶ÔÈ˦Â-actin£¨gene ID:60£¬ÒÔϼò³ÆACTB£©½øÐÐÒýÎïÉè¼ÆµÄʱºò£¬ÀûÓÃprimer-blast¶ÔÒýÎï·ÖÎöµÄʱºò·¢ÏÖÒýÎï³ýÁËÄÜÀ©Ôö³öACTBÍ⣬»¹ÄÜÀ©Ôö³öÒ»¸ö±àÂëPOTEêµ°°×Óò¼Ò×壨gene ID£º445582£©µÄ»ùÒò£¬ÓÚÊÇС³æ¶ÔÁ©»ùÒò½øÐÐÁËblast£¬½á¹û·¢ÏÖACTB cDNAÈ«ÐòÁÐÓëPOTEêµ°°×¼Ò×å»ùÒòCDNA²¿·ÖÐòÁÐÓÐמªÈ˵ÄÏàËÆ£¡ÏÂÃæÊÇÏêϸ½á¹û£º
    1.Ê×ÏÈ£¬ACTBºÍPOTEêµ°°×»ùÒòID²»Í¬£¬Ö¤Ã÷ÕâÍêÈ«ÊÇÁ½¸ö²»Í¬µÄ»ùÒò

ÕâÊÇACTBµÄID



ÕâÊÇPOTEµÄID
    2.Æä´Î£¬Á©»ùÒòblast½á¹ûÏÔʾACTBµÄÈ«CDNAÐòÁÐÓëPOTE²¿·ÖCDNAÐòÁÐÓÐמªÈ˵ÄÏàËÆ¶È

Á©»ùÒòµÄblast½á¹û
   3.ÔٴΣ¬ÕýÒòΪÁ©»ùÒòµÄÏàËÆ£¬ËùÒÔÔÚ¶ÔÈËACTB½øÐÐÒýÎïÉè¼ÆµÄʱºò×ÜÊÇÄÜÀ©Ôö³öPOTEµÄ²¿·ÖƬ¶Î£¬ËäÈ»À©Ôö²úÎï´óСÏàͬ½á¹¹Ò²¼«ÏàËÆ£¬µ«ÊÇÓÉÓÚËûÃÇÊÇÁ½¸ö²»Í¬µÄ»ùÒò£¬ËùÒÔÔÚÒÑACTB×÷ΪÄڲεÄʱºòʵ¼ÊÉÏÔö¼ÓÁ˳õʼģ°åÁ¿£¬Õâ¾Í²»ÊǶԶ¨Á¿²úÉú´íÎóÂð£¿

¶ÔÎÒÉè¼ÆµÄÈËACTBÒýÎï½øÐÐprimer-blast½á¹û£¬ÒòΪÆÈÓÚ±£ÃÜ£¬ËùÒÔδÉÏ´«ÎÒµÄÒýÎïÐòÁÐ


×îºó£¬»ùÓÚÎÒÅöµ½µÄÎÊÌ⣬ÓгæÓÑÄܽâÊÍÒ»ÏÂÂð£¿Í¬Ê±ÒòΪÕâ¸öÎÊÌâÈç¹ûµÃ²»µ½½âÊÍ»á¶ÔºÜ¶àÈ˵ĿÆÑÐÇé¿ö²úÉúÓ°Ï죬ËùÒÔ½¨Òé°æÖ÷½«´ËÌûÇ¿µ÷һϣ¬Ð»Ð»

[ Last edited by ËïÆôÁÁ on 2012-3-3 at 21:37 ]
»Ø¸´´ËÂ¥

» ÊÕ¼±¾ÌûµÄÌÔÌûר¼­ÍƼö

¡¾·Ö×ÓÉúÎï¡¿EPIÌû ÊÕ²Ø ÔÓÏî »ùÒò¿Î±í

» ±¾ÌûÒÑ»ñµÃµÄºì»¨£¨×îÐÂ10¶ä£©

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

» ÇÀ½ð±ÒÀ²£¡»ØÌû¾Í¿ÉÒԵõ½:

²é¿´È«²¿É¢½ðÌù

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

tianping

ľ³æ (СÓÐÃûÆø)


¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
Сľ³æ(½ð±Ò+0.5): ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
Î÷¹Ï: »ØÌûÖö¥ 2012-03-31 16:56:49
Î÷¹Ï: ½ð±Ò+5, MolEPI+1, ѧϰÁË~ 2012-03-31 16:57:14
³£Óÿ´¼Ò»ùÒò£¬±ÈÈç18s,gapdh, beta-actinµÈ£¬²¢²»Ò»¶¨ÊʺÏÓÃÀ´×ö¶¨Á¿£¬ÒѾ­ÓÐÐí¶àÎÄÕÂÌÖÂÛÕâ¸öÎÊÌâÁË¡£ÔÚ2003Äê×óÓÒоƬ¼¼ÊõÕýºìµÄʱºò£¬ÒѾ­ÓÐÎÄÕÂ̽ÌÖÈçºÎ´ÓоƬÊý¾ÝÖÐÍÚ¾ò¸ü¶àµÄ¿´¼Ò»ùÒò£¬Õâ·½ÃæµÄÎÄÕÂÐí¶à·¢±íÔÚbmc molecular biologyÉÏ£¬²¢ÇÒ¿ª·¢³öÁËgeNorm,NormFinder, BestKeeperµÈÈí¼þÀ´ÆÀ¹À×îÎȶ¨×îÊʺÏÓÃÀ´×öQPCR referenceµÄ»ùÒò¡£µ±È»£¬ÄÇЩÎÄÕµijö·¢µãÊdz£Óÿ´¼Ò»ùÒòÔÚijЩÌõ¼þϲ¢²»Îȶ¨£¬»òÕß´æÔÚparalog»áµ¼Ö²»ÊʺÏ×öreference¡£ÄãÕâ¶ùµÄ·¢ÏÖ¿ÉÄÜÓë»ùÒò´æÔÚparalogµÄÇé¿ö²î²»¶à£¬Èç¹ûactinÓëpoteÏàËÆÐÔÄÇô¸ß£¬ÓпÉÄÜÊÇhomolog£¬ÕâÖÖÇé¿öactin¿ÉÄܲ¢²»ÊʺÏ×ö¶¨Á¿µÄ¶ÔÕÕ»ùÒò¡£
16Â¥2012-03-14 23:57:54
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 26 ¸ö»Ø´ð
ÒýÓûØÌû:
Â¥: Originally posted by cindybrella at 2012-03-03 21:39:59:
°¡°¡°¡£¿

ÒÔÉÏÓÐÎÒÌṩµÄÁ©»ùÒòID£¬ÓÐÐËȤµÄ»°¿ÉÒÔ×Ô¼º²Ù×÷һϣ¬ÆÚÍûÓëÄãÉî¶È½»Á÷
3Â¥2012-03-03 21:41:53
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

xsm918

Ìú³æ (СÓÐÃûÆø)


¡ï
ËïÆôÁÁ(½ð±Ò+1):лл²ÎÓë
ÄãÉè¼ÆµÄÒýÎﻹ²»¹»ÌØÒ죡
4Â¥2012-03-04 01:58:11
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

exon2002

Ìú¸Ëľ³æ (ÖøÃûдÊÖ)


¡ï
ËïÆôÁÁ(½ð±Ò+1):лл²ÎÓë
¶ÔÂ¥Ö÷µÄϸÐıíʾºÜ¾´Å壡ÕâȷʵÊÇÒ»¸öÎÊÌâÁË¡£ÎҵĿ´·¨Óжþ£¬ÆäÒ»ÊÇÒª²éÒ»ÏÂPOTEÓëb-actinµÄ¹ØÏµ£¬¶þÕßÊÇ·ñÓÐÉúÎïѧͬԴÐÔ¡£À´×ÔÓÚͬһ¸ö»ùÒò»ò½ø»¯ÉϵIJ¿·Ö±£ÁôµÄÇé¿ö³£¼û¡£ÉõÖÁÔÚ·­Òëºó×öÁËijÖÖÐÞÊξͳÉÁËÁíÍâÒ»ÖÖµ°°×È¥ÐÐʹ²»Í¬»òÏàËÆµÄ¹¦ÄܵÄÇé¿öÒ²³£¼û¡£Æä¶þ£¬¼´Ê¹Á½¸öµ°°×µÄÐòÁм°ÆäÏàËÆ£¬Ê¹µÃÎÒÃǵÄÄڲβ»ÈçÒÔǰÏëÏóµÄÄÇôÇåÎú£¬µ«ÊÇÒÔ×ÜÌå×÷ΪÄÚ²ÎÒ²ÓÐЧ£¬Ö»ÊÇÆäÕæÕýµÄÀ´Ô´²»Ã÷ÀʶøÒÑ¡£Õâ¸öÒÉÎÊ¿ÉÒÔͨ¹ý»ùÒò±í´ïµ÷²é¶ø³ÎÇå¡£ËùÒÔÓÃb-actin×öÄڲεÄͬÈʲ»±Ø¾ª»Å¡£
5Â¥2012-03-04 05:39:31
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
¼òµ¥»Ø¸´
dongfang129Â¥
2012-03-04 14:52   »Ø¸´  
ËïÆôÁÁ(½ð±Ò+1):лл²ÎÓë
ËïÆôÁÁ12Â¥
2012-03-06 09:32   »Ø¸´  
ÒýÓûØÌû:
11Â¥: Originally posted by ˹¶à¸ñ¸ñ at 2012-03-06 09:15:44: Õâ¸öδÃâǣǿÁ˰ɣ¿

×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] ×Ü·Ö293Çóµ÷¼Á +6 ¼ÓÒ»Ò»¾Å 2026-03-25 9/450 2026-03-29 13:40 by ¼ÓÒ»Ò»¾Å
[¿¼ÑÐ] 375Çóµ÷¼Á +4 ÓêÏÄÕûÒ¹ 2026-03-29 4/200 2026-03-29 12:09 by Î޼ʵIJÝÔ­
[¿¼ÑÐ] 298Çóµ÷¼Á +3 ÖÖÊ¥´Í 2026-03-29 3/150 2026-03-29 12:06 by longlotian
[¿¼ÑÐ] 352·Ö-085602-Ò»Ö¾Ô¸985 +5 º£Äɰٴ¨Ly 2026-03-29 5/250 2026-03-29 09:57 by Sjndkwm
[¿¼ÑÐ] 0856Çóµ÷¼Á +7 —öèñ 2026-03-28 7/350 2026-03-29 08:28 by fmesaito
[¿¼ÑÐ] ѹ¹ú¼ÒÒ»ÇøÏߣ¬Çóµ¼Ê¦ÊÕÁô£¬Óж÷±ØÐ»£¡ +7 ÃÔÈ˵Ĺþ¹þ 2026-03-28 7/350 2026-03-28 16:47 by ´ß»¯´ó°×
[¿¼ÑÐ] ²ÄÁÏ277Çóµ÷¼Á +7 min3 2026-03-24 7/350 2026-03-28 11:39 by xuxiang
[¿¼ÑÐ] 085405 ¿¼µÄ11408Çó¸÷λÀÏʦ´ø×ß +3 Qiuѧing 2026-03-28 3/150 2026-03-28 09:19 by ÀֺǺǵÄ×·ÃÎÈË
[¿¼ÑÐ] ²ÄÁÏÇóµ÷¼ÁÒ»Ö¾Ô¸¹þ¹¤´ó324 +7 ãÆÐñ¶« 2026-03-28 9/450 2026-03-28 08:51 by Xu de nuo
[¿¼ÑÐ] 286Çóµ÷¼Á +4 ¶ªµôÀÁ¶è 2026-03-27 7/350 2026-03-28 08:07 by baoball
[¿¼ÑÐ] 285Çóµ÷¼Á +4 AZMK 2026-03-27 7/350 2026-03-27 20:59 by AZMK
[¿¼ÑÐ] ¿¼Ñе÷¼Á +4 Sanmu-124 2026-03-26 4/200 2026-03-27 17:49 by kiokin
[¿¼ÑÐ] 07»¯Ñ§280·ÖÇóµ÷¼Á +10 722865 2026-03-23 10/500 2026-03-27 15:51 by Plutoqq
[¿¼²©] 26É격 +3 ¼ÓÓͳ尡£¡ 2026-03-26 3/150 2026-03-27 15:38 by cls512
[¿¼ÑÐ] 292Çóµ÷¼Á +4 ÇóÇóÁËÊÕÏÂÎÒ°É£ 2026-03-26 4/200 2026-03-27 10:37 by zhshch
[˶²©¼ÒÔ°] ±±¾©ÁÖÒµ´óѧ˶µ¼ÕÐÉú¹ã¸æ +6 kongweilin 2026-03-26 8/400 2026-03-27 10:18 by FF_16
[¿¼ÑÐ] Çóµ÷¼Á£¬Ò»Ö¾Ô¸ ÄϾ©º½¿Õº½Ìì´óѧ´óѧ £¬080500²ÄÁÏ¿ÆÑ§Ó빤³Ìѧ˶ +4 @taotao 2026-03-26 5/250 2026-03-27 08:10 by hypershenger
[¿¼ÑÐ] ÉúÎïѧ 296 Çóµ÷¼Á +4 ¶ä¶ä- 2026-03-26 6/300 2026-03-26 19:01 by ²»³Ôô~µÄ؈
[¿¼ÑÐ] ²ÄÁϵ÷¼Á +3 iwinso 2026-03-23 3/150 2026-03-25 11:29 by greychen00
[¿¼ÑÐ] 300·Ö£¬²ÄÁÏ£¬Çóµ÷¼Á£¬Ó¢Ò»Êý¶þ +5 ³¬ÔÞµÄ 2026-03-24 5/250 2026-03-24 21:07 by ÐÇ¿ÕÐÇÔÂ
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û