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[求助]
求助F-actin和β-actin的区别 已有1人参与
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RT，只知道两个都是肌动蛋白，好像也是一样大小？ 咨询抗体公司，居然声称是一个蛋白？！ 我自己也讲不出区别，都是细胞骨架微丝的组成部分？f-actin好像集中在突起的边缘，伪足一样的…β-actin常作为内参，普遍表达…… 纠结的很，也查不到具体详细的比较，求专业的虫友指导！ 不胜感激！  |
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wizardfan
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感谢参与，应助指数 +1
林夕梦: 金币+10, ★★★很有帮助, 谢谢 2012-12-05 11:13:53
silicare: 金币+10, BioEPI+1, 应助指数+1, 谢谢参与 2013-04-22 08:51:01
感谢参与，应助指数 +1
林夕梦: 金币+10, ★★★很有帮助, 谢谢 2012-12-05 11:13:53
silicare: 金币+10, BioEPI+1, 应助指数+1, 谢谢参与 2013-04-22 08:51:01
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F和beta是2种不同的分类方法，看http://en.wikipedia.org/wiki/Actin In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins, found in muscletissues, are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility Individual subunits of microfilaments are known as globular actin (G-actin). G-actin subunits assemble into long filamentous polymers called F-actin. [ Last edited by wizardfan on 2012-12-5 at 06:55 ] |
7楼2012-12-05 06:53:19
myprayer
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★ ★ ★
感谢参与，应助指数 +1
wizardfan: 金币+3, BioEPI+1, 看上去是篇摘要，能把文章链接贴出就更好了，还有最好能高亮你的回答。最后活动期间给个EPI 奖励，以后多应助哈 2013-04-22 07:49:12
感谢参与，应助指数 +1
wizardfan: 金币+3, BioEPI+1, 看上去是篇摘要，能把文章链接贴出就更好了，还有最好能高亮你的回答。最后活动期间给个EPI 奖励，以后多应助哈 2013-04-22 07:49:12
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Background: Cells resident in certain hollow organs are subjected routinely to large transient stretches, including every adherent cell resident in lungs, heart, great vessels, gut, and bladder. We have shown recently that in response to a transient stretch the adherent eukaryotic cell promptly fluidizes and then gradually resolidifies, but mechanism is not yet understood. Principal Findings: In the isolated human bladder smooth muscle cell, here we applied a 10% transient stretch while measuring cell traction forces, elastic modulus, F-actin imaging and the F-actin/G-actin ratio. Immediately after a transient stretch, F-actin levels and cell stiffness were lower by about 50%, and traction forces were lower by about 70%, both indicative of prompt fluidization. Within 5min, F-actin levels recovered completely, cell stiffness recovered by about 90%, and traction forces recovered by about 60%, all indicative of resolidification. The extent of the fluidization response was uninfluenced by a variety of signaling inhibitors, and, surprisingly, was localized to the unstretch phase of the stretch-unstretch maneuver in a manner suggestive of cytoskeletal catch bonds. When we applied an ‘‘unstretch-restretch’’ (transient compression), rather than a ‘‘stretch-unstretch’’ (transient stretch), the cell did not fluidize and the actin network did not depolymerize. Conclusions: Taken together, these results implicate extremely rapid actin disassembly in the fluidization response, and slow actin reassembly in the resolidification response. In the bladder smooth muscle cell, the fluidization response to transient stretch occurs not through signaling pathways, but rather through release of increased tensile forces that drive acute disassociation of actin. β-actin作为一个亚基结构存在。前面有人说的有一点点道理，都是cytoskeletal里面的结构。 |
12楼2012-12-06 22:14:45
暮色风车
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2楼2012-12-04 11:39:07
3楼2012-12-04 12:33:18
hongqifei
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4楼2012-12-04 17:04:48
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