24小时热门版块排行榜

返回列表

本帖产生 2 个 BioEPI ，点击这里进行查看

当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖

林夕梦

木虫 (文坛精英)

珊瑚岛碧落堂主~~

应助: 4 (幼儿园)
贵宾: 0.293
金币: 3185
散金: 12492
红花: 87
沙发: 401
帖子: 10053
在线: 1564.4小时
虫号: 837704
注册: 2009-08-31
专业: 代谢性疾病药物药理

[求助] 求助F-actin和β-actin的区别已有1人参与

RT，只知道两个都是肌动蛋白，好像也是一样大小？
咨询抗体公司，居然声称是一个蛋白？！
我自己也讲不出区别，都是细胞骨架微丝的组成部分？f-actin好像集中在突起的边缘，伪足一样的…β-actin常作为内参，普遍表达……
纠结的很，也查不到具体详细的比较，求专业的虫友指导！
不胜感激！

回复此楼

» 猜你喜欢

许多奇迹，我们相信，才会实现……

1楼 2012-12-04 10:16:19

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

myprayer

专家顾问 (著名写手)

BioEPI: 4
应助: 81 (初中生)
贵宾: 2.822
金币: 32407.7
散金: 1696
红花: 158
沙发: 3
帖子: 2275
在线: 822.5小时
虫号: 1759711
注册: 2012-04-16
专业: 生物力学与生物流变学
管辖: 生物医药区

【答案】应助回帖

★ ★ ★
感谢参与，应助指数 +1
wizardfan: 金币+3, BioEPI+1, 看上去是篇摘要，能把文章链接贴出就更好了，还有最好能高亮你的回答。最后活动期间给个EPI 奖励，以后多应助哈 2013-04-22 07:49:12

Background: Cells resident in certain hollow organs are subjected routinely to large transient stretches, including every
adherent cell resident in lungs, heart, great vessels, gut, and bladder. We have shown recently that in response to a transient
stretch the adherent eukaryotic cell promptly fluidizes and then gradually resolidifies, but mechanism is not yet understood.
Principal Findings: In the isolated human bladder smooth muscle cell, here we applied a 10% transient stretch while
measuring cell traction forces, elastic modulus, F-actin imaging and the F-actin/G-actin ratio. Immediately after a transient
stretch, F-actin levels and cell stiffness were lower by about 50%, and traction forces were lower by about 70%, both indicative
of prompt fluidization. Within 5min, F-actin levels recovered completely, cell stiffness recovered by about 90%, and traction
forces recovered by about 60%, all indicative of resolidification. The extent of the fluidization response was uninfluenced by a
variety of signaling inhibitors, and, surprisingly, was localized to the unstretch phase of the stretch-unstretch maneuver in a
manner suggestive of cytoskeletal catch bonds. When we applied an ‘‘unstretch-restretch’’ (transient compression), rather than
a ‘‘stretch-unstretch’’ (transient stretch), the cell did not fluidize and the actin network did not depolymerize.
Conclusions: Taken together, these results implicate extremely rapid actin disassembly in the fluidization response, and slow actin
reassembly in the resolidification response. In the bladder smooth muscle cell, the fluidization response to transient stretch occurs
not through signaling pathways, but rather through release of increased tensile forces that drive acute disassociation of actin.
β-actin作为一个亚基结构存在。前面有人说的有一点点道理，都是cytoskeletal里面的结构。