24小时热门版块排行榜

返回列表

liusha80167

木虫 (正式写手)

应助: 0 (幼儿园)
金币: 3708.8
散金: 435
红花: 1
帖子: 608
在线: 71.6小时
虫号: 461664
注册: 2007-11-18
性别: MM
专业: 基因组学

[交流] 【求助/交流】求生物信息高手指教

今天做了个blastn 部分结果很奇怪，以前从来没遇到过，还请高手帮忙解决：
output文件：
BLASTN 2.2.18 [Mar-02-2008]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 1452_1848_129,28,+;3609_x1
      (28 letters)

Database: Publicknow_TE.gff.seq
         431,743 sequences; 90,834,805 total letters

Searching..................................................done

                                                               Score E
Sequences producing significant alignments:                   (bits) Value

scaffold1883_TransposonPrediction BGIBMKNOWNTEA00399324  Target ... 32 0.092
nscaf3026_TransposonPrediction BGIBMKNOWNTEA00303428  Target "L1... 32 0.092
nscaf2865_TransposonPrediction BGIBMKNOWNTEA00182017  Target "gy... 32 0.092
nscaf2948_TransposonPrediction BGIBMKNOWNTEA00242753  Target "L1... 30 0.28
                                    ...................................

>scaffold1060_TransposonPrediction BGIBMKNOWNTEA00389796  target "TRAS3-2" 4324 1  class "LINE-BM"_1_-
      Length = 1557

Score = 28.5 bits (17), Expect = 0.83
Identities = 4/19 (21%)
Strand = Plus / Plus

Query: 3    cattttaaggaaataattt 21
                              | | |       |
Sbjct: 458 tcggaaccagaatggggct 476

>nscaf2948_TransposonPrediction BGIBMKNOWNTEA00242753  Target "L1Bm-2" 193 298  class          "LINE-BM"_261_+
      Length = 108

Score = 30.1 bits (18), Expect = 0.28
Identities = 0/28 (0%)
Strand = Plus / Plus

Query: 1 tacattttaaggaaataattttaactag 28

Sbjct: 194                                           221

命令如下：nohup blastall -p blastn -i inputfile -o outputfile -d ./database -W 7 -q -1 -F F -I T &

所有文件均为fasta格式

回复此楼