求助!LC-ESI-MS自上而下法定量蛋白,不经过酶解是如何打出高价离子的?
我实验室的质谱仪是AB SCIEX 5500QTRAP,尝试过用纯蛋白,按文献中前处理的步骤和溶液比例进行检测,未果。文献中的质谱仪是Waters XEVO TQ-S triple quadrupole
请问各位大佬,是不是因为仪器是硬伤,还是因为其他一些参数呢?如何打出文献图中的高价母离子呢,对应的子离子是可以预测的吗?
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我实验室的质谱仪是AB SCIEX 5500QTRAP,尝试过用纯蛋白,按文献中前处理的步骤和溶液比例进行检测,未果。文献中的质谱仪是Waters XEVO TQ-S triple quadrupole
请问各位大佬,是不是因为仪器是硬伤,还是因为其他一些参数呢?如何打出文献图中的高价母离子呢,对应的子离子是可以预测的吗?
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文献中图


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图片2.png
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similar instrument sensitivity (Waters Xevo TQ-S sometimes maybe better, depend), You should see the protein different charge state envelop. fragment ions should be same.
vent and clean your instrument if sensitivity is low.
similar instrument sensitivity (Waters Xevo TQ-S sometimes maybe better, depend), You should see the protein different charge state envelop. fragment ions should be same.
vent and clean your instrument if sensitivity is low.