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关于肝微粒体代谢的四个问题,谢谢!

作者 shixianbao
来源: 小木虫 600 12 举报帖子
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问题1:You studied specific isoforms of CYP. Are you sure that these CYP isoforms are specifics to these biological activities?

问题2:- On the page 5, you precised that you added acetonitrile to stop the reaction. Why did you not use an acid to stop the reaction?

问题3:-Why UGT substrates were pre-incubated before inhibition with psoralidin

问题4:-Similarly, was there any pre-incubation of substrates or inhibitors for UGTs and CYPs? How long? And why?. Needs to be clarified in the text.

reviewer的4个问题,谢谢! 返回小木虫查看更多

今日热帖
  • 精华评论
  • 卢靖珊

    1.看指导文件

  • 卢靖珊

    2.有机溶剂可以破坏肝微粒体酶停止反应

  • 卢靖珊

    3.预孵育5min 再加入启动反应的液体,这样才是真正的反正时间

  • 卢靖珊

    哈哈 我个人见解啦~

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