关于肝微粒体代谢的四个问题,谢谢!
问题1:You studied specific isoforms of CYP. Are you sure that these CYP isoforms are specifics to these biological activities?
问题2:- On the page 5, you precised that you added acetonitrile to stop the reaction. Why did you not use an acid to stop the reaction?
问题3:-Why UGT substrates were pre-incubated before inhibition with psoralidin
问题4:-Similarly, was there any pre-incubation of substrates or inhibitors for UGTs and CYPs? How long? And why?. Needs to be clarified in the text.
reviewer的4个问题,谢谢!
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1.看指导文件
2.有机溶剂可以破坏肝微粒体酶停止反应
3.预孵育5min 再加入启动反应的液体,这样才是真正的反正时间
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哈哈 我个人见解啦~