24小时热门版块排行榜

返回列表

饿的神啊

新虫 (著名写手)

应助: 1 (幼儿园)
金币: 5982.7
散金: 122
帖子: 1004
在线: 66.3小时
虫号: 2477988
注册: 2013-05-23
性别: MM
专业: 植物病理学

[求助] DNASTAR分析测序结果已有1人参与

今天测序的结果返回了，是拼接过的，但是片段比我的目的片段大，是不是还含有载体序列？想通过DNAstar 的seqman去除载体序列，并拼接，用的是TransGen的pEASY-T1的载体，DNAstar中不包括，我自己添加了，但是设置不来啊，求帮助。pEASY-T1全长3928bp，说明书上描述如下：
LacZfragment: bases 1-547
M13 reverse priming site: bases 205-221
Multiple cloning site: bases 234-357
T7 promoter/priming site: bases 363-380
M13 Forward (-20) priming site: bases 388-403
f1 origin: bases 545-982
Kanamycin resistance ORF: bases 1316-2110
Ampicillin resistance ORF: bases 2128-2988
pUC origin: bases 3133-3806

以下是DNAstar里的帮助，我没太看懂啊
Follow the steps below to modify a vector definition in the Vector Catalog:
1 From the Project Menu, select Vector Catalog.
2 Use the mouse to select the name of the vector you wish to edit.
3 Click Edit to open the Vector Definition window.  Use the Tab key or the mouse to navigate between fields.
4 In the Vector field, edit the vector name.
5 Set a range for the vector sequence in the 5' and 3' coordinate boxes.  You may input negative numbers.  For instance, if the clone site were in position 75, you could enter -925 into the 5' box and 1075 into the 3' box to leave 1000 base pairs on either side without knowing the length of the vector. （我知道载体片段全长3928bp的话，那是应该设置-3928和3928吗？）

6 In In the Clone Site box, enter the coordinates of the clone site and a range in the  box.  If you know the precise clone site, enter the position and use a range of  1.  If you want a single definition for a number of sites in a polylinker, enter the midpoint position of the range of sites.  If you use multiple sites in the same vector for different projects, you may define a separate vector entry for each clone site, provided you give each definition a different vector name. （载体说明书上写的多克隆位点234-357，那这里应该怎么设置呢？）

7 Click OK to accept the modified definition.
求帮忙，纠结很久了，万分感谢！

vector.png

回复此楼