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章信来

木虫 (正式写手)

[求助] 可变剪切体RT-PCR检测的技术问题

各位虫友:
我有个基因存在不止一种类型可变剪切情况,当共同点是基因中间片段缺失,我想设计两端保守位的引物,采用RT-PCR的方法检测不同组织间的分布规律,如果都存在的话会同时扩增出几条大小不等的目的条带,RT-PCR结果则完全不是那么回事,所有组织只能扩增出一条最小的可变剪切体,其他的,尤其是未发生可变剪切的原始基因都扩增不出来,请各位虫友帮忙分析下原因和解决办法(PCR条件优化很多次了,不见任何改观),谢谢各位!
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aiguoss0902

新虫 (初入文坛)

引用回帖:
4楼: Originally posted by 章信来 at 2013-04-03 19:19:38
in fact, i haven't  solve this question yet, i want to do northern-blot, your suggestion maybe work, i will try it, thanks!
the big problem of AS is how to study the function, especially the differ ...

could you have solve the problem with northern-blot?
9楼2015-01-27 09:29:52
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cnb1987

银虫 (小有名气)

你好,我想问问,对于基因的不同剪切体如何鉴定?
2楼2013-04-02 16:19:22
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robustli

银虫 (小有名气)

【答案】应助回帖

How do you optimize your PCR condition? I have met the same thing. I use KOD to amplify my target gene. I only got the small fragment. Later I increased the denature and primer annealing time (from 20s to 25s). Interestingly, the bigger band appeared.
immunologyparasite
3楼2013-04-02 21:11:06
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章信来

木虫 (正式写手)

引用回帖:
3楼: Originally posted by robustli at 2013-04-02 21:11:06
How do you optimize your PCR condition? I have met the same thing. I use KOD to amplify my target gene. I only got the small fragment. Later I increased the denature and primer annealing time (from 2 ...

in fact, i haven't  solve this question yet, i want to do northern-blot, your suggestion maybe work, i will try it, thanks!
the big problem of AS is how to study the function, especially the different function to wild type gene, do you have any good idea?
4楼2013-04-03 19:19:38
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