4楼: Originally posted by 章信来 at 2013-04-03 19:19:38
in fact, i haven't  solve this question yet, i want to do northern-blot, your suggestion maybe work, i will try it, thanks!
the big problem of AS is how to study the function, especially the differ ...

3楼: Originally posted by robustli at 2013-04-02 21:11:06
How do you optimize your PCR condition? I have met the same thing. I use KOD to amplify my target gene. I only got the small fragment. Later I increased the denature and primer annealing time (from 2 ...