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Double Digestion with BamHI, NdeI
We recommend:
Buffer 2X Tango?
2-fold excess of BamHI
2-fold excess of NdeI
Incubate at 37¡ãC
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xuqingchun33(½ð±Ò+1): 2011-07-03 12:50:03
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Î÷¹Ï(½ð±Ò+5, EPI+1): Good!½²µÃºÜÇå³þÁË£¡ 2011-07-02 16:48:36
xuqingchun33(½ð±Ò+1): 2011-07-03 12:49:56
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Originally posted by xuqingchun33 at 2011-07-02 11:26:42:
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Double Digestion with BamHI, NdeI
We recommend:
Buffer 2X Tango?
2-fold excess of BamHI
2-fold excess of NdeI
Incubate at  ...

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Double Digestion with BamHI, NdeI¾ÍÊÇÑ¡ÔñµÄøÊÇBamHI, NdeI¡£
We recommend:
Buffer 2X Tango¸Ã¹«Ë¾ÍƼöʹÓõÄøÇлº³åÒºÊÇ2X Tango£¬¾ÍÊǼÓøÇÐÌåϵ×ÜÌå»ýµÄ1/5µÄ10X Tango¡£
2-fold  excess of BamHI
2-fold excess of NdeIÒòΪÕâÁ½¸öøÔÚ2X TangoÌõ¼þÏ»îÐÔ¶¼Ö»ÓÐ50-100%£¬²»ÊÇ100%£¬ÎªÁ˱£Ö¤ÇиîЧÂÊ£¬ËùÒÔ¼ÓÈëµÄøÁ¿ÊÇ2±¶¡£
Incubate at 37¡ãC Õâ¸ö¾ÍÊÇøÇÐζÈÁË¡£
4Â¥2011-07-02 16:09:34
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