±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 1628  |  »Ø¸´: 6
±¾Ìû²úÉú 2 ¸ö MolEPI £¬µã»÷ÕâÀï½øÐв鿴

dingjunrong

ľ³æ (СÓÐÃûÆø)

[ÇóÖú] PCR×ÜÊÇÎÞ·¨ÕýÈ·À©ÔöËùÐèÆ¬¶Î£¬ÎÊÌâÔÚÄÄ£¿

ÎÒÒªÀ©ÔöµÄÊÇÒ»¸öÊôµÄÌØÒìÐÔÆ¬¶Î1900bp,±¾À´ÒÔΪÒýÎïÓÐÎÊÌ⣬ÓÖºÏÁËÒ»±é£¬»¹ÊÇÀ©²»³ö£¬ÓôÃÆ°¡~
20ulÌåϵ£ºddH2O 14.1ul    10¡ÁPCR Buffer 2ul    dNTP 2ul  ÒýÎï¸÷0.5ul   Ä£°å0.5ul
TaqE 0.4 ul
PCR·´Ó¦³ÌÐò£º95¶È 5min 95¶È 1min   55¶È 1min  72¶È  3min  72¶È  10min
30¸öÑ­»·
Çó¸ßÈË·ÖÎö
µçӾͼÈçÏ£º´Ó×óÏòÓÒ£ºmarker10000,ÑôÐÔÑùÆ·£¬¿Õ°×¶ÔÕÕ¡£markerÌõ´øÓÉСÖÁ´ó£º250bp,500bp,1000bp,2000bp,.......
»Ø¸´´ËÂ¥

» ÊÕ¼±¾ÌûµÄÌÔÌûר¼­ÍƼö

¡¾·Ö×ÓÉúÎï¡¿EPIÌû

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

ÈËÉúÈçÏ·~Ï·ÈçÈËÉú
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

fhh819

Òø³æ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

1949stone(EPI+1): ¹ÄÀø 2011-05-11 12:41:36
dingjunrong(½ð±Ò+2): 2011-11-07 09:54:42
ÎÒ¿´ÁËÄãµÄ·´Ó¦ÌåϵµÄ³É·Öºó£¬¾õµÃ£º
1¡¢ÄãµÄÄ£°åÌ«ÉÙ£»ÊµÔÚ²»ÐÐÄ£°åÁ¿ÓÃ4ul¶¼¿ÉÒÔ¡£
2¡¢dNTPµÄŨ¶ÈÈç¹ûÊÇ100mMµÄ»°£¬ÓÃÁ¿¾ÍÖ»Òª0.4ul ¡£
3¡¢ÏàÐÅÍË»ðζȵÄÈ·¶¨£¬²»ÓÃ˵Á˰ɡ£²»¹ý¸öÈËÈÏΪÍË»ðζÈÓ°Ïì²»ÊǺܴó¡£
4¡¢Èç¹ûʵÔÚ²»ÐУ¬¾ÍÔÙ´ÎÉè¼ÆÒýÎï°É£¿¾¡Á¿ÕÒ±£ÊØÇøÉè¼ÆÒýÎï¡£
ÄãÊÔÒ»ÊÔÕâ¸ö·´Ó¦Ìåϵ°É£¿
20ulÌåϵ£º
ddH2O            14.4ul   
10¡ÁPCR Buffer      2ul   
dNTP                  0.4ul  
ÒýÎï                 ¸÷0.4ul   
Ä£°å                    2ul
TaqE                 0.4 ul
¾ã»³ÒÝÐË׳˼·É£¬ÓûÉÏÇàÌìÀ¿Ã÷Ô£¡
2Â¥2011-05-11 11:05:04
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

lly198784

ľ³æ (ÕýʽдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï ¡ï ¡ï
1949stone(½ð±Ò+4): Ö±½ÓʹÓÃÊÔ¼ÁºÐ 2011-05-11 12:41:59
dingjunrong(½ð±Ò+3): 2011-05-12 09:35:24
ÎÒÒ²Êǿˡһ¸ö3000¶àbpµÄ»ùÒò£¬ÓõÄÊǸùÁö¾ú»ùÒò×éÄ£°å£¬PCR³öÀ´ºÜ¶à·ÇÌØÒìÐÔÌõ´ø¡£ÎÒ¾õµÃ²»ÊÇÌåϵµÄÎÊÌ⣬²»¹ýÎÒÓõÄøÊÇpyrobest¾ÛºÏø£¬ÒÔǰPCR¶¼ÊÇÓõÄÕâ¸öø£¬ÒÔǰµÄÄ£°åÊǴ󳦸˾ú»ùÒò×飬Õâ´ÎµÄ²»ÖªµÀÔõô»ØÊ¡£Òª²»Äã»»¸öøÊÔÊÔ¡£
3Â¥2011-05-11 11:21:45
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

lly198784

ľ³æ (ÕýʽдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï
1949stone(½ð±Ò+2): 1·ÖÖÓÒ²¿ÉÒÔ 2011-05-11 12:42:29
dingjunrong(½ð±Ò+3): лл 2011-05-12 09:35:37
ÄãµÄÑÓÉìʱ¼äÌ«³¤ÁË£¬2min¾Í¹»ÁË£¬ÍË»ðζÈÊʵ±Ìá¸ß¡£
4Â¥2011-05-11 11:24:18
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ÌìʹÍÐ

ר¼Ò¹ËÎÊ (Ö°Òµ×÷¼Ò)

T.Shen

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï ¡ï
dhd997(½ð±Ò+3, EPI+1): 2011-05-11 17:03:38
dingjunrong(½ð±Ò+2): 2011-11-07 09:54:59
1£ºÌåϵÖÐDNTP¼ÓµÄÓеã¶àÁË£¬50ulÌåϵÖвÅÌí¼Ó1ul£»
2£ºÓÐûÓÐÉèÒ»¸öζÈÌݶȣ¬Èç¹ûûÓУ¬¿ÉÒÔÉèÖÃÒ»¸öζÈÌݶȣ¬¸ù¾ÝÄãÁ½ÌõÒýÎïµÄµÄTMÖµ£¬ºÍ³ýÒÔ5£¬È»ºóÉÏϼӼõ¼¸¶ÈÊÔÊÔ£¬¿´¿´ÄÄÒ»¸öζÈÏÂP³öÀ´µÄ×îÁÁ¡£
3£ºÑÓÉìʱ¼ä3minÓе㳤°É£¿ÄãµÄÄ¿µÄƬ¶Î1.9kb£¬×î¶à2min¾Í¿ÉÒԸ㶨¡£¡£¡£
Whenever,Wherever,Anyway!MustRemember:Fight!KeepFighting!NeverGiveUp!
5Â¥2011-05-11 16:53:21
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

syn1985

ľ³æ (ÕýʽдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

ÑôÐÔÑùÆ·¾ÍÊÇÄã˵µÄPCR²úÎï°É ÔÚPCR·´Ó¦ÌåϵÀïÃæ¼ÓµãDMSO ÍË»ðζÈÔÚÌá¸ßµã¾Í¿ÉÒÔÁË DMSO»áÃ÷ÏÔ¼õÉÙ·ÇÌØÒìÐÔÀ©ÔöÌõ´øµÄ
6Â¥2011-05-17 17:55:14
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

»÷ÖÐÔÂÁÁ

гæ (³õÈëÎÄ̳)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï ¡ï
Î÷¹Ï(½ð±Ò+3): Good£¡ 2011-05-18 18:16:59
Ä£°åŨ¶ÈÒ²»áÓ°ÏìPCR ½á¹ûµÄ£¡Ó¦¸ÃÊÔ×ÅÃþË÷Ò»ÏÂ×îÊʵÄÄ£°åŨ¶È¡£
7Â¥2011-05-18 16:15:33
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ dingjunrong µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 0854Çóµ÷¼Á +4 assdll 2026-04-05 4/200 2026-04-06 12:29 by ÖзÉÔº¿Õ¹ÜѧԺÑ
[Óлú½»Á÷] 3-äåßÁà¤-4-¼×È©ºÏ³É 10+3 Ñ©¸âÒ²Äܱ¬Õ¨ 2026-03-31 9/450 2026-04-06 00:24 by nBuï®
[¿¼ÑÐ] 308Çóµ÷¼Á +4 maverick^_^ 2026-04-03 4/200 2026-04-05 19:08 by À¶ÔÆË¼Óê
[¿¼ÑÐ] 323Çóµ÷¼Á +8 Àî¼ÑÀÖ1 2026-04-04 8/400 2026-04-04 22:26 by hemengdong
[¿¼ÑÐ] 341Çóµ÷¼Á +3 Âå¶àÂÞ 2026-04-02 4/200 2026-04-04 21:36 by ÖÇÄÜÖÇ»Û
[¿¼ÑÐ] 11408 Ò»Ö¾Ô¸Î÷µç£¬277·ÖÇóµ÷¼Á +4 zhouzhen654 2026-04-03 4/200 2026-04-04 18:10 by Öí»á·É
[¿¼ÑÐ] 22408Çóµ÷¼Á 354·Ö ¿É¿çרҵ +3 hannnnnnn 2026-04-04 3/150 2026-04-04 14:35 by ÍÁľ˶ʿÕÐÉú
[¿¼ÑÐ] 268Çóµ÷¼Á +8 ÄãºÃtg 2026-04-03 9/450 2026-04-04 05:08 by gswylq
[¿¼ÑÐ] ѧ˶288µ÷¼Á!!! +3 СÍõxw123 2026-04-03 3/150 2026-04-03 21:20 by à£à£à£0119
[¿¼ÑÐ] Ò»Ö¾Ô¸Äϲý´óѧ324Çóµ÷¼Á +13 hanamiko 2026-04-01 13/650 2026-04-03 18:30 by lsÁõ˧
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤µ÷¼ÁÒ»Ö¾Ô¸´óÁ¬º£ÊÂ085600£¬349 +11 ³ÔµÄ²»ÉÙ 2026-03-30 11/550 2026-04-03 18:05 by Jimmyandyou
[¿¼ÑÐ] 338Çóµ÷¼Á +4 zzz£¬£¬r 2026-04-03 4/200 2026-04-03 16:39 by lijunpoly
[¿¼ÑÐ] 274Çóµ÷¼Á +9 ˳Àí³ÉÕÅ 2026-04-03 10/500 2026-04-03 15:10 by °¡¿¡£¡
[¿¼ÑÐ] ±§Ç¸ +5 ÌïºéÓÐ 2026-03-30 5/250 2026-04-03 10:24 by linyelide
[¿¼ÑÐ] ½»Í¨ÔËÊ俼ÊÔ264·ÖÇ󹤿Ƶ÷¼Á +4 jike777 2026-04-02 4/200 2026-04-02 21:53 by zllcz
[¿¼ÑÐ] Ò»Ö¾Ô¸ÉϺ£º£Ñó´óѧ083200ʳƷѧ˶£¬Çóµ÷¼Á£¬½ÓÊÜÆäËûרҵ +6 whatÕÅ 2026-04-01 7/350 2026-04-02 16:48 by zzsw+
[¿¼ÑÐ] Ò»Ö¾Ô¸±±¾©¿Æ¼¼£¬085601×Ü·Ö305Çóµ÷¼Á +9 °ëÉú¹Ï£¡ 2026-04-01 11/550 2026-04-02 08:28 by Wang200018
[¿¼ÑÐ] Ò»Ö¾Ô¸°²»Õ´óѧ¼ÆËã»ú¿ÆÑ§Óë¼¼Êõѧ˶£¬331·ÖÇóµ÷¼Á +5 ½¯²ýÅôqtj 2026-04-01 5/250 2026-04-02 08:10 by fxue1114
[¿¼ÑÐ] 322Çóµ÷¼Á +8 ÈýË®sss 2026-04-01 8/400 2026-04-01 10:19 by ÌÆãå¶ù
[¿¼ÑÐ] Ò»Ö¾Ô¸Öк£Ñó320»¯Ñ§¹¤³ÌÓë¼¼Êõѧ˶Çóµ÷¼Á +8 ÅûÐÇºÓ 2026-03-30 8/400 2026-03-31 08:53 by lbsjt
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û