大修 审稿意见求助
There is no indication that the xx gene transcription levels were normalized to a housekeeping gene wholes transcription does not change in this population. The Methods state that the "housekeeping gene GAPDH was used as an internal control to confirm success of the RT reaction". Typically the HK gene is used to normalized quantity of sample used for the gene of interest as well as a QC measure.
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我这英语水平啊
大侠在吗？
The authors do not described why they consider a 1 % change （研究组3.68%, NC组 4.7%）in DNA methylation levels in the xx gene promoter is considered a biologically relevant change. What is the biological mechanism responsible for how this minor change in DNA methylation could lead to the detected changes in expression，
reviewer好像认为nadph不是HK（house keeping)基因