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———————— 关于LB琼脂的疑问

作者 CCZ2008
来源: 小木虫 100 2 举报帖子
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试验室预购LB琼脂,想选Amresco的,但Amresco的LB Agar分两种,分别是
LB Agar, Lennox Formulation
LB Agar, Miller Formulation

请问这两种的区别是什么?应用范围? 返回小木虫查看更多

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  • xjxlovelxl

    配方不一样,后者的氯化钠含量比较高

    LB Broth, Miller contains twenty times the sodium chloride level
    of Luria Broth Base, Miller and twice the level found in LB Broth,
    Lennox.3,5,6 This allows the researcher to select the optimal salt
    concentration for a specific strain in LB Agar, Miller.

    https://www.bd.com/ds/technicalCenter/inserts/LB_Agar_Lennox.pdf
    https://www.bd.com/ds/technicalCenter/inserts/LB_Agar_Miller.pdf

    Lennox Formulation
    Approximate Formula* Per Liter
    Tryptone ...................................................................10.0 g
    Yeast Extract .............................................................. 5.0 g
    Sodium Chloride ........................................................ 5.0 g
    Agar .........................................................................15.0 g
    ——————————————————————————————

    Difco™ LB Agar, Miller
    Approximate Formula* Per Liter
    Tryptone ...................................................................10.0 g
    Yeast Extract .............................................................. 5.0 g
    Sodium Chloride ...................................................... 10.0 g
    Agar .........................................................................15.0 g,

  • xjxlovelxl

    引用回帖:
    Originally posted by CCZ2008 at 2010-09-16 11:32:00:
    试验室预购LB琼脂,想选Amresco的,但Amresco的LB Agar分两种,分别是
    LB Agar, Lennox Formulation
    LB Agar, Miller Formulation

    请问这两种的区别是什么?应用范围?

    您找的是哪家代理,我们实验室也想购买amersco的化学试剂

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