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———————— 关于LB琼脂的疑问
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试验室预购LB琼脂,想选Amresco的,但Amresco的LB Agar分两种,分别是 LB Agar, Lennox Formulation LB Agar, Miller Formulation 请问这两种的区别是什么?应用范围? |
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CCZ2008(金币+9, 博学EPI+1):多谢! 2010-09-16 14:25:11
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配方不一样,后者的氯化钠含量比较高 LB Broth, Miller contains twenty times the sodium chloride level of Luria Broth Base, Miller and twice the level found in LB Broth, Lennox.3,5,6 This allows the researcher to select the optimal salt concentration for a specific strain in LB Agar, Miller. https://www.bd.com/ds/technicalCenter/inserts/LB_Agar_Lennox.pdf https://www.bd.com/ds/technicalCenter/inserts/LB_Agar_Miller.pdf Lennox Formulation Approximate Formula* Per Liter Tryptone ...................................................................10.0 g Yeast Extract .............................................................. 5.0 g Sodium Chloride ........................................................ 5.0 g Agar .........................................................................15.0 g —————————————————————————————— Difco™ LB Agar, Miller Approximate Formula* Per Liter Tryptone ...................................................................10.0 g Yeast Extract .............................................................. 5.0 g Sodium Chloride ...................................................... 10.0 g Agar .........................................................................15.0 g |

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