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饿的神啊

新虫 (著名写手)

[求助] 急求!核苷酸序列翻译成氨基酸后,怎么回到对应的核苷酸序列? 已有2人参与

下载了一段核苷酸序列,通过NCBI的ORF finder找了ORF,后来又比对找到了保守域,现在知道这个保守域的氨基酸序列,我想找到对应的最初的核苷酸序列应该怎么办呢?附上序列
最初的核苷酸序列
>Cs8g12000.3 transcript(len:3478) -964.74
CAGAAATGCATAGACATTTTAAAGAGATTAGACAGCCGCTAACTGTTTGATAATAATCTCTTTTTCTCCATGCAAATATATATCACTCAA
AATTAGTAACATACGAGTAGTCTTTCAATTGACTGAAAATTCTTCATATTCATTCATTCATTCATTCAATTTCATGAAAAAAATGAGAAG
CGAGGAGGGCAGAGGAGGCTATATTGAAAACAATTCAGATACTGAAAGTATTGGCAGTGACAAAAGCGCATTCAGCGGACCGCTTGCCAA
TAAACGAGCTTCCAAGAAAAGCGCCCGGTTCAATCTTCCGCCAGAAGCAGCAGTCACAAGCAGCAGTAACAATAACGATGATGGTGGTTA
TGTAGAGATCACACTGGACATACGTGATGACTCTGTCGCAGTTCACAGCGTTCAGGGCGCACTTCATCATGAAGATCCTGAGTTGTCACT
GTTGGCCAAGAAGACTTTGGAGATTAATAATAAATCTTCATCATTTCGGTCGTCTTTGTTCAGAAACACTTCCTCGCATATCAAGCAGGT
CTCTCAAGAGCTCAAACGCTTTGCTTCCTTGACACGGCGACCCAGCAGCAGGCGTTTTGATAGGACTAAATCCGCTGCAGCTCATGCTCT
TAAAGGACTCAAGTTCATCACCACCAAGACGGGAGCTGCCGGAAATGGCTGGCCTGCCGTCGAGAAGCGGTATAACGAGCTCGCCATCAC
GACCTCCGACGGCCTTCTTCATTGCTCGATGTTCGGCGAATGCATAGGGATGAACAAGGAGTCTAAAGAATTTGCGGGTGAGCTATTCCG
AGGACTTTGTAGAAAGCATAATATAAGTGGTGATTCTATCAACAAAGCTCAGCTCAAGGAGTTTTGGGACCAGATTTCCGACGAGAGCTT
TGACTCCAGGCTCCAAACTTTCTTTGACATGGTCGACACAGACGCTGATGGAAGAATCACTGAAGAAGAAGTCAAAGAGATTATTAGTCT
GAGTGCTTCTGCAAACAAGCTCTCCAACATTCAGAAACAAGCAGAGGAATATGCAGCCATGATTATGGAAGAATTAGACCCTGACAATGC
CGGATACATCATGATTCATAACCTGGAAACATTGTTGTTGCAAGCTCCAAATCAATCTGTAAGAGTAGGAGACAGTCGAATTCTGAGTCA
AATGTTGAGCCAGAAGCTGAAGCCAACCCAAGAAAACAACCCATTACGAAAATGTTATCAGAAGATTAAGTACTTTTTGATGGATAATTG
GCAAAGGGTTTGGATTATGCTGTTGTGGCTTGGAATTGTTGGGGGTTTATTTGCCTACAAGTTTGTGCAGTACCGAAACAAGGCTGCTTA
TGATGTGATGGGCTATTGCGTTTGCATTGCTAAAGGAGGAGCAGAGACACTTAAATTCAACATGGCCTTGATATTATTACCAGTTTGCCG
AAACACCATCACATGGCTCAGAAACAAAACTAAATTAGGACTTGTTGTCCCTTTCGATGACAACTTAAATTTCCATAAGGTGATCGCGGT
TGGAATTGGGATTGGAGTTATATTACACGGCGGAGCTCATCTAACCTGTGATTTCCCGCGACTCCTCCACGCGACTGAAGAAGAATACGA
GCCAATGAAGCCTTACTTTGGGGACGAGCAACCGGATAATTACTGGTGGTTTGTAAAGGGAGTTGAAGGAGTAACAGGGATCATAATGGT
GGTGTTAATGGTGATAGCATTCACTTTGGCAACGCCTTGGTTCAGGCGCAACAAGCTCAATCTGCCAAAACCACTTAAAAGACTCACTGG
ATTCAATGCCTTTTGGTACTCACATCATCTTTTCATCATTGTCTATACCCTTCTCATTGTCCATGGAATTAAACTCTATCTCACCAAGAA
ATGGTATCAGAAGACGACATGGATGTATCTGGCTGTCCCGATGGTTCTTTATGCTTGTGAGAGATTGACTAGAGCTTTGAGATCAAGCAT
CGAGCCTGTCAAGATTCTCAAGGTTGCTGTTTATCCTGGAAATGTCTTGGCATTACACATGTCAAAACCACAAGGCTTCAAATACAAGAG
CGGACAGTACATGTTTGTAAATTGCGCTGCCGTCTCACCATTCGAATGGCATCCGTTTTCCATCACATCATCACCAGGAGATGACTACCT
CAGCGTTCACATCCGAACTTTGGGCGATTGGACTAGGCAGCTTAAGACAGTTTTCTCTGAGGTTTGCCAGCCTGCACCTGCTGGGAAGAG
CGGTCTTCTCAGAGCAGAAAGGGAAAATAACTCTCGAGGGTATGTATTATTTGTTTTTTCCTTTTTAATAATTCATCAATGAAATTAATG
TAAAATATTTGACAGAATGTTGACATAGATAAGATAAGACAAATACCAATTTAATTACTTAACTAAAAAATAAAGTAAATCTCAACTAAT
TTCGCTTATATTTCTGTTACCGTTCTATCAAGATTCTGTTTGTGTCTTTCTCCTTTTTTGAATTAAAATTTATATCTTGTGATGTAGCAG
GTTTCCAAAGATATTAATAGACGGACCATATGGAGCACCAGCTCAAGACTATAAAAAATATGATGTAGTGCTACTAGTGGGGCTTGGAAT
TGGGGCTACTCCCATGATCAGCATAGTCAAAGACATCATCAACAACATGAAATCAGAAGACAACAATTTAGAGTCAGGATTAACAGTAAA
CAACAACAACAAGAATTCATCATTCAATACAAGAAAAGCATACTTCTATTGGGTGACAAGGGAACAAGGTTCATTCGAGTGGTTCAAAGG
CATAATGAATGAGGTGGCGGAGATGGATGAGAAGAGGGTAATAGAATTACACAATTATTGCACAAGTGTGTACGAAGAAGGAGATGCCAG
GTCAGCGCTTATAGCTATGCTTCAATCACTCCATCATGCAAAGAATGGTGTTGATGTTGTTTCTGGGACACGTGTCAAATCTCACTTTGC
TAAGCCAAATTGGAGACAAGTTTACAAGAGGATTGCTCTTCATCATCCTGATTCTAGAATTGGAGTATTCTATTGCGGTGCACCCGCACT
AACAAAGGAGCTAAGACAACTAGCCTCGGATTTCTCCCACAAGACTTCCACTAAGTTTGAATTTCATAAAGAAAATTTTTAAAATTAATT
AATTTATAGCTTCATCAATTGATTAATAATATTATTCTTGAACTTGGAAAGTTTTTTCTTTCTTTAGAAAGGAAGGTCCTCGGACATTAG
TAGCCACTTGCAAATAGAAAATACGTACACTCAAATGGTCTGACTTTTCTTTTCTCTTTTTTTAATCACCCTTTTAAAAAATAAAGAGAG
TGATGCCCCTTCTAGTCTTCTAGAAGAGTTGTTCAGTTTTATAGATATTCCAAAGTCCAATCTTACAGCTGTTCAACGTATTGTGTAATT
CAATTCTTTCTTAATTGTTGACTGAATTGAATATGTAATGTAAACATATTCAGAGCTC
这个是ORF
>Cs8g12000.3   722aa  NADPH-OX superfamily; EFh superfamily; EF-hand-7; Ferric-reduct superfamily; FNR-like superfamily
MKKMRSEEGRGGYIENNSDTESIGSDKSAFSGPLANKRASKKSA
                     RFNLPPEAAVTSSSNNNDDGGYVEITLDIRDDSVAVHSVQGALHHEDPELSLLAKKTL
                     EINNKSSSFRSSLFRNTSSHIKQVSQELKRFASLTRRPSSRRFDRTKSAAAHALKGLK
                     FITTKTGAAGNGWPAVEKRYNELAITTSDGLLHCSMFGECIGMNKESKEFAGELFRGL
                     CRKHNISGDSINKAQLKEFWDQISDESFDSRLQTFFDMVDTDADGRITEEEVKEIISL
                     SASANKLSNIQKQAEEYAAMIMEELDPDNAGYIMIHNLETLLLQAPNQSVRVGDSRIL
                     SQMLSQKLKPTQENNPLRKCYQKIKYFLMDNWQRVWIMLLWLGIVGGLFAYKFVQYRN
                     KAAYDVMGYCVCIAKGGAETLKFNMALILLPVCRNTITWLRNKTKLGLVVPFDDNLNF
                     HKVIAVGIGIGVILHGGAHLTCDFPRLLHATEEEYEPMKPYFGDEQPDNYWWFVKGVE
                     GVTGIIMVVLMVIAFTLATPWFRRNKLNLPKPLKRLTGFNAFWYSHHLFIIVYTLLIV
                     HGIKLYLTKKWYQKTTWMYLAVPMVLYACERLTRALRSSIEPVKILKVAVYPGNVLAL
                     HMSKPQGFKYKSGQYMFVNCAAVSPFEWHPFSITSSPGDDYLSVHIRTLGDWTRQLKT
                     VFSEVCQPAPAGKSGLLRAERENNSRGYVLFVFSFLIIHQ
这个是我找到的保守域的核苷酸:148-248 aa  TKSAAAHALKGLKFITTKTGAAGNGWPAVEKRY NELAITTSDGLLHCSMFGECIGMNKESKEFAGELFRGLCRKHNISGDSINKAQLKEFWDQ ISDESFDS:
611-685 aa   ILKVAVYPGNVLALHMSKPQGFKYKSGQYMFVNCAAVSPFEWHPFSITSSPGDDYLSVHI RTLGDWTRQLKTVFS
我想找到这个保守域氨基酸对应的原核苷酸中的位置,好设计引物!
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liuderong

铁杆木虫 (正式写手)

【答案】应助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感谢参与,应助指数 +1
饿的神啊: 金币+20, ★★★★★最佳答案, 十分感谢! 2015-07-03 14:53:39
用NCBI的blastX,http://blast.ncbi.nlm.nih.gov/Bl ... p;LINK_LOC=blasttab。query那个框(上面那个)粘贴所有的DNA序列,另一个粘贴蛋白,比对一下就知道了。
3楼2015-07-03 11:31:37
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stone2239

铁杆木虫 (著名写手)

【答案】应助回帖

感谢参与,应助指数 +1
两个保守域在你核酸序列中的对应区段分别是:605-907,1994-2218

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2楼2015-07-03 11:30:27
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饿的神啊

新虫 (著名写手)

送红花一朵
引用回帖:
2楼: Originally posted by stone2239 at 2015-07-03 11:30:27
两个保守域在你核酸序列中的对应区段分别是:605-907,1994-2218

金币送人了,给你送朵花吧,谢谢你的帮助!
5楼2015-07-03 14:54:37
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