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amisking: ½ð±Ò+1, ¹ÄÀø·¢Ìû½»Á÷£¡ 2013-10-22 18:26:53
ø 1ul
buffer 2ul
Ä£°å 1ul
ÒýÎï ¸÷3ul
ddH2O 10ul
dNTP 5ul
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mrchen8903(amisking´ú·¢): ½ð±Ò+2, ¸Ðл·¢ÌûÓ¦Öú£¬°ïÖú³æ×Ó 2013-10-22 18:26:46
using Takara Ex Taq,  follow their recommended protocol.

Takara Ex Taq™ DNA Polymerase (5 U/¦ÌL)                        0.125 ¦ÌL
10X Buffer (20 mM Mg2+ plus)                        2.5 ¦ÌL
dNTP Mix (2.5 mM each)                        2.0 ¦ÌL
DNA template                                1 ¦ÌL (2-200 ng)
Primer 1 (5 µM stock)                                1 ¦ÌL
Primer 2 (5 µM stock)                                1 ¦ÌL
Sterile ddH20                                                  17.375 ¦ÌL
Total volume                                                                   25 ¦ÌL

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