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An ideal cellular probe for practical application should minimally perturb living systems at the concentrations employed. Accordingly, the cytotoxicity of solvent INR1 and INR2 were determined using the MTT assay. In general, at a compound concentration of 5 ¦ÌM, cellular viability was estimated as greater than 90% after incubation for 2 h (Fig. S3), and higher concentration (10¦ÌM) can result in decreased cell survival, 76% for INR1 and 85% for INR2. The results indicate that the probes generally present low toxicity for living cell imaging under the conditions applied.

[ Last edited by zhtear99 on 2013-5-13 at 20:08 ]
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The ideal cellular probe which is used in practice is suggested to interfere with living systems at a certain concentration. Therefore we measured the quantities of cytotoxicity factor £¬namely INR1 and INR2£¬ by using MTT assay. After incubates 2h£¬ the cellular viability is usually more than 90%  at 5 ¦ÌM. Whereas cell survival will reduce to 76% and 85% for INR1 and INR2£¬respectively £¬while the concentration is increase to 10 ¦ÌM. The result show that the probe possesses low toxicity for living cell under the condition just mentioned above.
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An ideal  cellular probe which used in practice should minimize the disturbance to the concentration of biological systems, therefore, we applied MTT to measure cytotoxicity as well as solvent INR1 and INR2. In general, at 5 ¦ÌM concentration of a  compound , cellular viability was estimated as greater than 90%, however, it will decreased to 76% to 85% for solvent INR1 and INR2 because of the high concentration(10¦ÌM) after 2h incubation (fig.3).  The result indicates that the probes generally present under the condition of low toxicity for living cell imaging.
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