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tutufei

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mafang68

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lstt09nk(½ð±Ò+1): ¸Ðл²ÎÓë 2011-03-16 09:47:27
tutufei(½ð±Ò+1): лÁË 2011-03-21 21:22:37
RNAÖÊÁ¿¿ÉÒÔ²âOD260£¨ºÃÏñÊÇ£¬¼Ç²»ÇåÁË£©Öµ£¬ÔÚ1.8-2.0¼äºÃ
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2Â¥2011-03-16 09:38:26
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tangchaoxi

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tutufei(½ð±Ò+1): ²»ÊÇ£¬ÊÖÌáŶ 2011-03-21 21:23:28
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Originally posted by tutufei at 2011-03-16 09:24:02:
1.RNAµçÓ¾ÐèҪעÒâÄÄЩ£¬ÐèҪʲôÄÚ²ÎÂð£¬ÎÒÏëÒª¿´ÏÂRNAÌáÈ¡µÄÖÊÁ¿Çé¿ö
2.ÈçºÎÈ¥³ýRNAµÄDNAÎÛÈ¾ÄØ£¬ÎÒÒª½øÒ»²½×öRT-PCR,È»ºóÀ©ÔöÄ¿µÄÌõ´ø£¬Èç¹ûÓÐDNAÎÛȾ£¬¾Í¶¼ÄÜÀ©³öÀ´ÁË

RNAµçÓ¾ÒªÓÃеĽººÍеĵçÓ¾»º³åÒº£¬µçÓ¾²ÛʲôµÄÏ´¸É¾»¾Í¿ÉÒÔÁË£¬RNAµÄÖÊÁ¿Ö÷Òª»¹ÊÇRNAÌáÈ¡¹ý³Ì¾ö¶¨µÄ¡£ÏñÂ¥ÉÏ˵µÄÄÇÑù¾ÍÐÐÀ²¡£ÄãÊÇÓÃÊÔ¼ÁºÐÂð£¿
3Â¥2011-03-16 09:41:55
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nininini

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°¿Óî(½ð±Ò+1): ²»´í£¬Ð»Ð»½»Á÷£¬DNAµÄÏû»¯Ó¦¸Ã»¹ÓÐÒ»²½³éÌᣬ²¹¼ÓDEPCË®µ½400ul£¬ÒÔÂȷ³éÌá¾ÍÐÐÁË 2011-03-16 23:57:21
tutufei(½ð±Ò+8): лл 2011-03-21 21:35:36
µçÓ¾ÈçÂ¥ÉÏËù˵Ӧ¸ÃûÎÊÌ⣬µ±È»ÓãģţУô¦Àí¹ýµÄË®ÖÆ½º¼°»º³åÒºÓ¦¸Ã»á¸üºÃ¡«¡«£Ò£Î£ÁÖÊÁ¿¿ÉÒÔͨ¹ýOD260/280£¬±ÈÀýÔÚ1.8~2.ÔÙ¾ÍÊǵçÓ¾¿´Í¼ÁË£¬28s/18s»ò23s/16s´óԼΪ2:1,
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4Â¥2011-03-16 10:18:26
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tutufei

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Originally posted by tangchaoxi at 2011-03-16 09:41:55:
RNAµçÓ¾ÒªÓÃеĽººÍеĵçÓ¾»º³åÒº£¬µçÓ¾²ÛʲôµÄÏ´¸É¾»¾Í¿ÉÒÔÁË£¬RNAµÄÖÊÁ¿Ö÷Òª»¹ÊÇRNAÌáÈ¡¹ý³Ì¾ö¶¨µÄ¡£ÏñÂ¥ÉÏ˵µÄÄÇÑù¾ÍÐÐÀ²¡£ÄãÊÇÓÃÊÔ¼ÁºÐÂð£¿

ÊÖÌáµÄ£¬ÓñðÈËʵÑéÊÒµÄÊÔ¼Á£¨º¹£¡£©£¬È˼ÒÓÃbiozolÊÔ¼Á´¦ÀíÖ²Î²»ÖªµÀÄܲ»ÄÜÓÃÀ´´¦Àíϸ¾ú£¬ÌýËµÕæºËµÄmRNA±ÈÔ­ºËµÄÎȶ¨ÊÇÂð£¬ÎÄÏ×ÉÏÓÐ˵ϸ¾úmRNA°ëË¥ÆÚ¾Í¼¸·ÖÖÓ£¬¿´È˼ÒÌáÖ²ÎïRNAºÜ´Öáî°¡£¬¸úÌáÒ»°ãµÄDNAûÁ½Ñù£¬ÅܵçÓ¾Ò²ÊǸúÒ»°ãµÄÒ»Ñù£¬°¥£¬ºÃ²»·ÅÐÄ£¬»¹ÓÐÓÐÊÔ¼ÁºÐ˵Ã÷Êé±êÃ÷ϸ¾úÓÃÁ¿1*107--5*108´Î·½¸öÊÇʲô¸ÅÄºÃ¶àÎÊÌâ°¡£¬Âé·³´ó¼ÒÀ²
5Â¥2011-03-16 23:51:05
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zhangby2002

Òø³æ (СÓÐÃûÆø)


tutufei(½ð±Ò+1): лÁË 2011-04-24 18:50:29
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Originally posted by tutufei at 2011-03-16 09:24:02:
1.RNAµçÓ¾ÐèҪעÒâÄÄЩ£¬ÐèҪʲôÄÚ²ÎÂð£¬ÎÒÏëÒª¿´ÏÂRNAÌáÈ¡µÄÖÊÁ¿Çé¿ö
2.ÈçºÎÈ¥³ýRNAµÄDNAÎÛÈ¾ÄØ£¬ÎÒÒª½øÒ»²½×öRT-PCR,È»ºóÀ©ÔöÄ¿µÄÌõ´ø£¬Èç¹ûÓÐDNAÎÛȾ£¬¾Í¶¼ÄÜÀ©³öÀ´ÁË

Ê×ÏÈRNAÌáÈ¡Ê±ÌØ±ð×¢ÒâÒª·ÀÖ¹ÎÛȾ£¬Äã¿ÉÒÔÓúËË㶨Á¿ÒÇ¿´¿´ODֵѽ¡£Ò»°ã2×óÓÒ£¨+¡ª0.1£©£¬¿ÉÒÔµçÓ¾¼ì²âһϣ¬Ö÷Òª¿´ÓдøÂ𣿲»ÐèÒªÄڲΰɣ¬Äã¿ÉÒÔÓÃB-actin²ÎÕÕһϡ£µçÓ¾½á¹ûÄã¿ÉÒÔ¿´³ö£¬Ò»Ìõ´ø±È½Ï¸É¾»¡£×ö·´×ªÂ¼ÊÔÑéʱ×öºÃÒª×öÈ¥³ýRNAÖÐDNAµÄÎÛȾÕâ²½£¬¼ÓDNAø1£¬DNAbuffer,DEPC,È»ºóÔÙ¼ÓEDTA.
6Â¥2011-03-19 17:06:28
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wangleisdnu

½ð³æ (ÕýʽдÊÖ)


tutufei(½ð±Ò+5): thank you very much! 2011-03-21 21:32:42
ÒýÓûØÌû:
Originally posted by tutufei at 2011-03-16 23:51:05:
ÊÖÌáµÄ£¬ÓñðÈËʵÑéÊÒµÄÊÔ¼Á£¨º¹£¡£©£¬È˼ÒÓÃbiozolÊÔ¼Á´¦ÀíÖ²Î²»ÖªµÀÄܲ»ÄÜÓÃÀ´´¦Àíϸ¾ú£¬ÌýËµÕæºËµÄmRNA±ÈÔ­ºËµÄÎȶ¨ÊÇÂð£¬ÎÄÏ×ÉÏÓÐ˵ϸ¾úmRNA°ëË¥ÆÚ¾Í¼¸·ÖÖÓ£¬¿´È˼ÒÌáÖ²ÎïRNAºÜ´Öáî°¡£¬¸úÌáÒ»°ãµÄDNAûÁ½ ...

Trizol can be used to isolate RNA from E coli  107-8 means od600 ---1-10
normaly 1 ml od600 =1 means you have 107 cells
everything should be new and freshly prepared,
I have more experience in RNA, if you have more questions just contact me
7Â¥2011-03-21 19:03:47
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wuw579

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1¡¢×îºÃÓñäÐÔ½º  ¿ÉÒÔÉÏÍø²é
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8Â¥2011-03-21 20:36:43
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tutufei

Í­³æ (СÓÐÃûÆø)


ÒýÓûØÌû:
Originally posted by nininini at 2011-03-16 10:18:26:
µçÓ¾ÈçÂ¥ÉÏËù˵Ӧ¸ÃûÎÊÌ⣬µ±È»ÓãģţУô¦Àí¹ýµÄË®ÖÆ½º¼°»º³åÒºÓ¦¸Ã»á¸üºÃ¡«¡«£Ò£Î£ÁÖÊÁ¿¿ÉÒÔͨ¹ýOD260/280£¬±ÈÀýÔÚ1.8~2.ÔÙ¾ÍÊǵçÓ¾¿´Í¼ÁË£¬28s/18s»ò23s/16s´óԼΪ2:1,
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9Â¥2011-03-21 21:30:51
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ls2046

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lxjwishes

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11Â¥2011-03-22 09:49:16
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Eva2009

½û³æ (³õÈëÎÄ̳)

tutufei(½ð±Ò+1): лÁË 2011-04-24 18:48:40
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12Â¥2011-03-22 11:17:48
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nininini

Òø³æ (СÓÐÃûÆø)


tutufei(½ð±Ò+1): лл 2011-04-24 18:48:30
ÒýÓûØÌû:
Originally posted by tutufei at 2011-03-21 21:30:51:
£Ò£Î£ÁOD260/280ÊÇÓÃÆÕͨµÄ×ÏÍâ·Ö¹â¹â¶È¼Æ¼ì²âÂð£¿ÊµÑéÊҵıÈÉ«Ãó×îÉÙÈÝÁ¿ÊÇ1ml£¬¶øÌáÈ¡µÄRNAÁ¿ºÜÉٵİ¡

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13Â¥2011-03-23 10:01:51
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szylnl

Òø³æ (СÓÐÃûÆø)


tutufei(½ð±Ò+2): лл 2011-04-24 18:47:26
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