Znn3bq.jpeg
²é¿´: 1592  |  »Ø¸´: 7
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

lilirong2009

Í­³æ (СÓÐÃûÆø)


[½»Á÷] ¡¾ÇóÖú/½»Á÷¡¿ÒýÎïµÄPCR TmÖµ Çë½Ì

ÎÒÉè¼ÆÁ˼¸ÖÖÒýÎ³¤¶È¶¼Îª18bp£¬Óù«Ê½ Tm=4(G+C)+2(A+T)-5¡æ  ¼ÆËãµÃTmֵΪ43¡æ×óÓÒ£¬ÄÇPCRʱ°´Ëã·¨ Tm-5¡æ ÉèÍË»ðζÈʱ£¬ÍË»ðζȾÍÖ»ÓÐÈýÊ®¼¸ÉãÊ϶ÈÁË£¬ÕâÑùÉèµÄ»°ÍË»ðζÈÊDz»ÊÇÌ«µÍ£¿ºÏÀíÂð£¿ÔõôÉèÖÃÍË»ðζȱȽϺÏÀí°¡£¿
ÎÒÆäÖÐÒ»¶ÔÒýÎïΪ 5¡®-AAGACTCCTATCACTATG-3¡¯  Tm 45¡æ
                             5¡®-TAAAAGATTTGTGGCATA-3¡¯  Tm 41¡æ

       ллָ½Ì!

[ Last edited by lilirong2009 on 2010-11-17 at 17:27 ]
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

» ÇÀ½ð±ÒÀ²£¡»ØÌû¾Í¿ÉÒԵõ½:

²é¿´È«²¿É¢½ðÌù

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Îҵľ­Ñ鹫ʽ£º50mM [Na+]µÄTmÖµ¼ÓÉÏ5~10¶È£¬×î½ü×öÁËÒ»´Î£¬¼Óµ½15¶È¶¼ÄÜÀ©³öÀ´
8Â¥2010-11-18 00:07:27
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 8 ¸ö»Ø´ð

lij2

½ð³æ (СÓÐÃûÆø)


ËãµÄʱºò²»Óüõ5£¬Êµ¼ÊÓõÄʱºò¼õ
2Â¥2010-11-17 18:53:32
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

zjzz

ľ³æ (ÕýʽдÊÖ)


3Ä©¶Ë×îºÃ²»ÒªÊÇA°É
3Â¥2010-11-17 21:17:33
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

anik

Òø³æ (ÕýʽдÊÖ)


Ϊʲô²»ÓÃOligo6Èí¼þÄØ£¿
4Â¥2010-11-17 21:49:26
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 282£¬µçÆø¹¤³Ìרҵ£¬Çóµ÷¼Á£¬²»Ìôרҵ +5 jggshjkkm 2026-04-10 5/250 2026-04-10 10:37 by cal0306
[¿¼ÑÐ] Ò»Ö¾Ô¸ÖÐÄÏ´óѧÎïÀíѧ£¬Ó¢Ò»66£¬Çóµ÷¼Á +4 ³¤ÑÌì½ì» 2026-04-08 5/250 2026-04-10 10:31 by Ó±¹û¶ù
[¿¼ÑÐ] ¿¼ÑжþÂÖµ÷¼Á +8 ¹ÊÈË?? 2026-04-09 8/400 2026-04-10 09:44 by Çà÷duoduo
[¿¼ÑÐ] 278Çóµ÷¼Á +27 ·¶æÃÄÈ 2026-04-07 31/1550 2026-04-09 20:49 by zhouxiaoyu
[¿¼ÑÐ] 297Çóµ÷¼Á +22 ljy20040718£¡ 2026-04-03 24/1200 2026-04-09 20:48 by yanenwang
[¿¼ÑÐ] »¯¹¤Çóµ÷¼Á£¡ +34 RichLi_ 2026-04-06 34/1700 2026-04-09 20:39 by zhouxiaoyu
[¿¼ÑÐ] µ÷¼Á +19 2261744733 2026-04-08 19/950 2026-04-09 19:11 by vgtyfty
[¿¼ÑÐ] 291·Öµ÷¼Á +3 Éϰ¶Ð¡Ó¨¼ÓÓÍ 2026-04-09 4/200 2026-04-09 17:28 by 1753564080
[¿¼ÑÐ] 0860004 Çóµ÷¼Á 309·Ö +6 Yin DY 2026-04-09 6/300 2026-04-09 10:19 by °¡Àî999
[¿¼ÑÐ] »¯Ñ§308·ÖÇóµ÷¼Á +21 ÄãºÃÃ÷ÌìÄãºÃ 2026-04-07 23/1150 2026-04-08 22:32 by ¿­¿­Òª±ä˧
[¿¼ÑÐ] ²ÄÁÏ¿ÆÑ§Ó빤³Ì320Çóµ÷¼Á£¬080500 +12 »Æ¹ÏζÊíÆ¬ 2026-04-06 12/600 2026-04-08 16:26 by luoyongfeng
[¿¼ÑÐ] 0703µ÷¼Á£¬Ò»Ö¾Ô¸Ìì½ò´óѧ319·Ö +23 haaaabcd 2026-04-05 26/1300 2026-04-08 16:19 by luoyongfeng
[¿¼ÑÐ] 288»·¾³×¨Ë¶,Çóµ÷²ÄÁÏ·½Ïò +35 lllllos 2026-04-04 39/1950 2026-04-07 23:24 by Ò»Ö»ºÃ¹û×Ó?
[¿¼ÑÐ] 301Çóµ÷¼Á +18 121. 2026-04-04 18/900 2026-04-07 17:49 by À¶ÔÆË¼Óê
[¿¼ÑÐ] ÇóÖú +3 ¿¨¿¨¶«88 2026-04-06 4/200 2026-04-06 15:28 by going home
[¿¼ÑÐ] ÉúÎïѧ308·ÖÇóµ÷¼Á£¨Ò»Ö¾Ô¸»ª¶«Ê¦´ó£© +8 ÏàÐűػá¹ââÍòÕ 2026-04-05 10/500 2026-04-05 12:19 by Hdyxbekcb
[¿¼ÑÐ] ÊýÒ»Ó¢Ò»274»úеµ÷¼Á +5 ÐÇÔÉÁ÷ϼ 2026-04-04 6/300 2026-04-05 11:38 by arrow8852
[¿¼ÑÐ] 357Çóµ÷¼Á +13 1050389037 2026-04-03 13/650 2026-04-03 22:27 by Î޼ʵIJÝÔ­
[¿¼ÑÐ] 322Çóµ÷¼Á +4 FZAC123 2026-04-03 4/200 2026-04-03 20:55 by zhq0425
[¿¼ÑÐ] 372·Ö²ÄÁÏÓ뻯¹¤£¨085600£©Ò»Ö¾Ô¸ºþÄÏ´óѧÇóµ÷¼Á +3 À¶¼ãƬ 2026-04-03 4/200 2026-04-03 17:58 by Jimmyandyou
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û