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[½»Á÷] ¡¾ÇóÖú/½»Á÷¡¿RNAÖÐDNAÎÛȾÎïµÄÈ¥³ý FermentasµÄDNase I

fermentas µÄDNASE1 RNA×îºÃÓÃDNase´¦Àíһϣ¬ÓÚÊǶ©¹ºÁËFermentasµÄDNase I£¬£¨ÒòÎªÄæ×ªÂ¼ºÐ×ÓÒ²ÊÇFermentas£©¿ÉÒ»¿´ËµÃ÷ÓеãɵÑÛ£¬±¾À´ÊÇÏëÕÒµã½Ý¾¶£¬¼ò»¯DNaseÏû»¯£¬¿ÉÊÇÌåϵÊÇÕâÑù½¨ÒéµÄ£º
Add to an RNase-free tube:
RNA 1¦Ìg
10X reaction buffer with MgCl2 1¦Ìl
DEPC-treated Water (#R0601) to 9¦Ìl
Deoxyribonuclease I (DNase I), RNase-free (1u/¦Ìl) 1¦Ìl (1u)
Note
If larger amounts of DNA-free RNA are required, the reaction
mixture should be scaled up.
Ribonuclease inhibitor (typically 1u/¦Ìl) can also be included in
the reaction mixture to prevent RNA degradation.
Incubate at 37¡ãC for 30 minutes.
Add 1¦Ìl 25mM EDTA and incubate at 65¡ãC for 10 minutes. RNA hydrolyzes during heating in the absence of a chelating agent (4).
Use the prepared RNA as a template for reverse
transcriptase.
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Template RNA total RNA 0.1-5 ¦Ìg
oligo (dT)18 primer 1 ¦Ìl
DEPC-treated water to 12 ¦Ìl
Total volume 12 ¦Ìl
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