求助审稿意见回复,谢谢大家!!!
谢谢大家!!!
审稿人说我在introduction部分的讨论有失公允,因为我指出HPLC等方法仪器昂贵,操作复杂,而我使用的荧光检测法的仪器造价更贵,约25000$。
随后我在网上查了一下价格发现,国内的荧光计价格在几万—几十万不等,并没有审稿人说的那么昂贵,我应该如何解释呢?因为不知道审稿人是否为中国人,外国的荧光计普遍是更贵的。另外,我在大量文献中也看到有关HPLC方法仪器昂贵,操作复杂的描述,我可以怎么回复审稿人呢?直接贴出这些文献吗?
这是审稿人的评价:
Introduction: The authors write that other methods are expensive, complex, tedious, time-consuming, and require complex sample pretreatment or need expensive equipment. I do not agree. The other methods (HPLC or chemiluminescence) are simple and inexpensive. These methods are not "sophisticated" but can be routinely performed by technicians. Also, they do not require a complex nanomaterial to be prepared. Fluorometry is particularly expensive, in contrast. Fluorometers do not come for less than 25,000 $ and require substantial operational skills. The authors should discuss existing methods in a more balanced way.
这是我的论文:
Many detection methods are sensitive and specific, like high performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS),ultra-fast liquid chromatography and photodiode array detection ,and chemiluminescence (CL) . But they are expensive, time-consuming, and demand well-trained operators and complex pretreatment. All these negative characters limit their application. Therefore, a fast, low-cost and approachable method is vital for food quality and market surveillance.
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你的写法有问题。液相等方法也很实用,使用也很简单,在医药方面使用最为广泛。你用荧光检测器检测,还得借助液相,再说荧光的检测的条件必须是有荧光的物质。荧光检测器用的少,确实比紫外更贵。相当于一台液相的价格。
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多看看文献的比较。根据你检测的材料体现方法的优越性。
hplc是极其常规的方法且便宜