1. How was the sample size chosen to ensure adequate power to
detect a pre-specified effect size? (Give section/paragraph or page #)
For animal studies, include a statement about sample size estimate
even if no statistical methods were used.
2. Describe inclusion/exclusion criteria if samples or animals were
excluded from the analysis. Were the criteria pre-established?
(Give section/paragraph or page #)
3. If a method of randomization was used to determine how samples/
animals were allocated to experimental groups and processed,
describe it. (Give section/paragraph or page #)
For animal studies, include a statement about randomization even if no
randomization was used.
4. If the investigator was blinded to the group allocation during the
experiment and/or when assessing the outcome, state the extent of
blinding. (Give section/paragraph or page #)
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