1，Figure layout and scaling： Electronic figures should be sized to fit on single 8.5" × 11" or A4 paper, preferably at 1(3.5 in), 1.5 (5.0 in) or 2 (7.3 in) columns wide. In laying out information in a figure, the objective is to maximize the space given to presentation of the data. Avoid wasted white space and clutter.
这个要求8.5" × 11" 直接就看不懂了，关于后面尺寸的描述实在没看懂。。。求大神解答。

2，Color-mix and contrast considerations：
Avoid using red and green together. Color blind individuals will not be able read the figure
因为荧光染色有红有绿，难道都不能使用要重新拍

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