FN        Thomson Reuters Web of Science™
VR        1.0
PT        J
AN        25553973
DT        Journal Article
TI        Enhancement of l-phenylalanine production by engineered Escherichia coli using phased exponential l-tyrosine feeding combined with nitrogen source optimization.
AU        Yuan, Peipei
Cao, Weijia
Wang, Zhen
Chen, Kequan
Li, Yan
Ouyang, Pingkai
SO        Journal of bioscience and bioengineering
VL        120
IS        1
PS        36-40
PY        2015
PD        2015 Jul (Epub 2014 Dec 29)
LA        English
AB        Nitrogen source optimization combined with phased exponential l-tyrosine feeding was employed to enhance l-phenylalanine production by a tyrosine-auxotroph strain, Escherichia coli YP1617. The absence of (NH4)2SO4, the use of corn steep powder and yeast extract as composite organic nitrogen source were more suitable for cell growth and l-phenylalanine production. Moreover, the optimal initial l-tyrosine level was 0.3gL(-1) and exponential l-tyrosine feeding slightly improved l-phenylalanine production. Nerveless, l-phenylalanine production was greatly enhanced by a strategy of phased exponential l-tyrosine feeding, where exponential feeding was started at the set specific growth rate of 0.08, 0.05, and 0.02h(-1) after 12, 32, and 52h, respectively. Compared with exponential l-tyrosine feeding at the set specific growth rate of 0.08h(-1), the developed strategy obtained a 15.33% increase in l-phenylalanine production (l-phenylalanine of 56.20gL(-1)) and a 45.28% decrease in l-tyrosine supplementation. Copyright 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
C1        State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, People's Republic of China.
SS        Index Medicus
ID        Engineered Escherichia coli; Fed-batch; Nitrogen source; l-Phenylalanine; l-Tyrosine feeding
SN        1347-4421
JC        100888800
PA        Japan
SA        In-Data-Review
RC        05 Jun 2015
PE        29 Dec 2014
DI        10.1016/j.jbiosc.2014.12.002
UT        MEDLINE:25553973
ER       
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