配制一定数量的梯度浓度，进液相，一般检测限峰高是基线噪音峰高的3倍，定量限是基线噪音峰高的10倍

可以参考《GB/T 5009.1-2003 食品卫生检验方法 理化部分 总则》 的附录A部分。

计算出不同浓度标样作出的标准曲线的标准偏差Sy/x，并将其假定为空白标准偏差，其3倍及10倍值被假定为估计的LOD和LOQ

这里的LOD和LOQ也可认为是仪器的LOD和LOQ，即IDL和IQL

ICH:
QL = 10 σ  /S
    DL=3.3σ  /S
where  σ  = the standard deviation of the response
          S = the slope of the calibration curve
The slope S may be estimated from the calibration curve of the analyte. The estimate of σ  may be carried out in a variety of ways for example

LOQetermination of the signal-to-noise ratio is performed by comparing measured signals from samples with known low concentrations of analyte with those of blank samples and by establishing the minimum concentration at which the analyte can be reliably quantified. A typical signal-to-noise ratio is 10:1.
LODetermination of the signal-to-noise ratio is performed by comparing measured signals from samples with known low concentrations of analyte with those of blank samples and establishing the minimum concentration at which the analyte can be eliably detected. A signal-to-noise ratio between 3 or 2:1 is generally considered acceptable for estimating the detection limit，