Znn3bq.jpeg
²é¿´: 3138  |  »Ø¸´: 12

chunqizzm

Òø³æ (СÓÐÃûÆø)


[½»Á÷] SDS-PAGEÑùƷŨËõµÄ·½·¨

SDS-PAGEÑùÆ·ÐèҪŨËõ´ó¼ÒÓõÄÊÇʲôÊÔ¼Á£¨ÀýÈ磺TCA£©£¿¾ßÌå²½Ö裿

[ Last edited by chunqizzm on 2012-3-25 at 23:36 ]
»Ø¸´´ËÂ¥

» ÊÕ¼±¾ÌûµÄÌÔÌûר¼­ÍƼö

µ°°×ÖÊʵÑé¼¼Êõ

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹ØÉ̼ÒÍÆ¼ö: (ÎÒÒ²ÒªÔÚÕâÀïÍÆ¹ã)

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

» ÇÀ½ð±ÒÀ²£¡»ØÌû¾Í¿ÉÒԵõ½:

²é¿´È«²¿É¢½ðÌù

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
»ØÌûÖ§³Ö ( ÏÔʾ֧³Ö¶È×î¸ßµÄǰ 50 Ãû )

zxc6884

Ìú¸Ëľ³æ (ÖøÃûдÊÖ)


¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
chunqizzm: ½ð±Ò+5, ÓаïÖú£¡Ð»Ð»£¡£¡²»ÖªµÀÄãÓмÆËã¹ýÕâÖÖ·½·¨µÄÑùÆ·»ØÊÕÂÊûÓУ¿ 2012-03-26 21:40:40
chunqizzm: »ØÌûÖö¥ 2012-03-26 21:40:44
ÕâÊÇÎÒÒ»Ö±ÓõÄTCA·½·¨
Normal TCA
To eliminate TCA soluble interferences and protein concentration
1) To a sample of protein solution add Trichloroacetic acid (TCA) 100% to get 13% final concentration. Mix and keep 5min ¨C20C and then 15min 4C; or longer time at 4C without the ¨C20C step for lower protein concentration. Suggestion: leave ON if the protein concentration is very low.
1) (preparation of 100% TCA: 454ml H2O/kg TCA. Maintain in dark bottle at 4C.Be careful, use gloves!!!).
2) Spin 15min 4C in microfuge at maximum speed (15000g). Carefully discharge supernatant and retain the pellet: dry tube by inversion on tissue paper (pellet may be difficult to see).
3) For PAGE-SDS, resuspend samples in a minimal volume of sample buffer. (The presence of some TCA can give a yellow color as a consequence of the acidification of the sample buffer ; titrate with 1N NaOH or 1M TrisHCl pH8.5 to obtain the normal blue sample buffer color.)
6Â¥2012-03-26 21:07:16
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

zxc6884

Ìú¸Ëľ³æ (ÖøÃûдÊÖ)


¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
chunqizzm: ½ð±Ò+5 2012-03-27 07:21:19
chunqizzm: »ØÌûÖö¥ 2012-03-27 07:21:45
ÒýÓûØÌû:
6Â¥: Originally posted by zxc6884 at 2012-03-26 21:07:16:
ÕâÊÇÎÒÒ»Ö±ÓõÄTCA·½·¨
Normal TCA
To eliminate TCA soluble interferences and protein concentration
1) To a sample of protein solution add Trichloroacetic acid (TCA) 100% to get 13% final concentr ...

Õâ¸öÊÇ×î³£ÓõÄTCA³Áµí·½·¨£¬Ò»°ãÐèÒªµ°°×ÔÚ5¦Ìg/mlÒÔÉϲÅÄܳÁÏÂÀ´£¬Ò²¾ÍÊÇ˵´ó¸Å»¹ÓÐ5¦Ìg/ml×óÓÒµ°°×²»ÄܳÁµíÀ´£¬ÕâÊÇÀíÂ۵ģ¬Êµ¼ÊÊÕÂÊÓëÑùÆ·ºÍ²Ù×÷Ïà¹Ø£¬Èç¹ûÄãÑùÆ·±ÈÕâŨ¶È»¹µÍµÄ»°¿ÉÒÔÊÔÏÂTCA-DOC³Áµí£¬ÄܳÁµí1¦Ìg/mlÒÔϵĵ°°×£¬È»ºóÒøÈ¾¡£

TCA-DOC
For precipitation of very low protein concentration
1) To one volume of protein solution, add 1/100 vol. of 2% DOC (Na deoxycholate, detergent).
2) Vortex and let sit for 30min at 4C.
3) Add 1/10 of Trichloroacetic acid (TCA) 100% vortex and let sit ON at 4C (preparation of 100% TCA: 454ml H2O/kg TCA. Maintain in dark bottle at 4C.Be careful, use gloves!!!).
4) Spin 15min 4C in microfuge at maximum speed (15000g). Carefully discharge supernatant and retain the pellet: dry tube by inversion on tissue paper (pellet may be difficult to see). [OPTION: Wash pellet twice with one volume of cold acetone (acetone keep at ¨C20C). Vortex and re-pellet samples 5min at full speed between washes].
5) Dry samples under vacuum (speed vac) or dry air. For PAGE-SDS, resuspend samples in a minimal volume of sample buffer. (The presence of some TCA can give a yellow colour as a consequence of the acidification of the sample buffer; titrate with 1N NaOH or 1M TrisHCl pH8.5 to obtain the normal blue sample buffer color.)

» ±¾ÌûÒÑ»ñµÃµÄºì»¨£¨×îÐÂ10¶ä£©

9Â¥2012-03-26 22:04:27
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
ÆÕͨ»ØÌû

zlu520

ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)


Öù²ãÎö ͸Îö
2Â¥2012-03-25 23:17:06
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

chunqizzm

Òø³æ (СÓÐÃûÆø)


ÒýÓûØÌû:
2Â¥: Originally posted by zlu520 at 2012-03-25 23:17:06:
Öù²ãÎö ͸Îö

»á²»»áºÜ·Ñʰ¡£¿ÄÜŨËõ50»ò100±¶Âð£¿
3Â¥2012-03-25 23:43:09
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

mingxie

ľ³æ (СÓÐÃûÆø)


¡ï
Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
ÓÐŨËõÆ÷µÄ»°³¬Â˰ɣ¬±È½Ï¼ò±ã
4Â¥2012-03-26 09:13:32
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

xingnian0925

½ð³æ (СÓÐÃûÆø)


¿ÉÒÔÊÔÊÔ³¬Â˹ܡ£
5Â¥2012-03-26 09:16:16
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

chunqizzm

Òø³æ (СÓÐÃûÆø)


ÒýÓûØÌû:
4Â¥: Originally posted by mingxie at 2012-03-26 09:13:32:
ÓÐŨËõÆ÷µÄ»°³¬Â˰ɣ¬±È½Ï¼ò±ã

ŨËõÆ÷£¿Äܲ»Äܸø¸öÏà¹Ø½éÉܵÄÁ´½Ó°¡£¡ÊDz»ÊÇGEÂôµÄÄÇÖÖ°¡£¿
7Â¥2012-03-26 21:42:52
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

chunqizzm

Òø³æ (СÓÐÃûÆø)


ÒýÓûØÌû:
5Â¥: Originally posted by xingnian0925 at 2012-03-26 09:16:16:
¿ÉÒÔÊÔÊÔ³¬Â˹ܡ£

лл£¡SDS-PAGEµçÓ¾ÑùÆ·±¾À´¾ÍÉٵϰ£¬Óó¬Â˹ܿÉÄܾÍÐв»Í¨ÁË¡£
8Â¥2012-03-26 21:46:56
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

chunqizzm

Òø³æ (СÓÐÃûÆø)


ËÍÏÊ»¨Ò»¶ä
ÒýÓûØÌû:
9Â¥: Originally posted by zxc6884 at 2012-03-26 22:04:27:
Õâ¸öÊÇ×î³£ÓõÄTCA³Áµí·½·¨£¬Ò»°ãÐèÒªµ°°×ÔÚ5¦Ìg/mlÒÔÉϲÅÄܳÁÏÂÀ´£¬Ò²¾ÍÊÇ˵´ó¸Å»¹ÓÐ5¦Ìg/ml×óÓÒµ°°×²»ÄܳÁµíÀ´£¬ÕâÊÇÀíÂ۵ģ¬Êµ¼ÊÊÕÂÊÓëÑùÆ·ºÍ²Ù×÷Ïà¹Ø£¬Èç¹ûÄãÑùÆ·±ÈÕâŨ¶È»¹µÍµÄ»°¿ÉÒÔÊÔÏÂTCA-DOC³Áµí£¬ÄܳÁ ...

ºÜÓаïÖú£¡£¡Äܲ»ÄÜ·¢Ò»ÏÂÕâÖÖ·½·¨µÄ³ö´¦°¡£¿£¨ÎÄÏ×»ò×ÊÁÏ£©chunqizzm@126.com  »¹ÓоÍÊÇ£¬TCA-DOC·½·¨ÖÐDOC£¨ÍÑÑõµ¨ËáÄÆ£©Æðʲô×÷ÓÃÖªµÀÂ𣿣¿µÚ4²½ÖÐOPTIONµÄÒªÓõ½±ûͪÊDz»ÊDZØÐëÒªÕâÒ»²½°¡£¿
±¾À´ÊÇÏëÔÙËÍJBµÄ¿ÉÊÇÒ»¸öÈËÖ»ÄÜËÍ10¸ö£¡
10Â¥2012-03-27 07:32:48
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

wen1023tao

Í­³æ (СÓÐÃûÆø)


µÍÎÂÀä¶³¸ÉÔï
11Â¥2012-03-27 11:36:06
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

liveinºÏ·Ê

Ìú¸Ëľ³æ (ÕýʽдÊÖ)


¡ï
Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
ÒýÓûØÌû:
2Â¥: Originally posted by zlu520 at 2012-03-25 23:17:06:
Öù²ãÎö ͸Îö

½è´Ë±¦µØÇë½ÌÒ»¸öÎÊÌ⣺Ïë°ÑÏËÎ¬ËØÃ¸½øÐд¿»¯£¬Ó¦¸ÃÔõô×öÄØ£¿Ëù·ÖÀëµÄø¶ÔÆÏ¾ÛÌÇÄý½ºÓзֽâÄÜÁ¦£¬ÄÜÓÃÇíÖ¬ÌÇÄý½º´úÌæÂð£¿
12Â¥2012-03-27 16:14:18
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

shashaɳ

Í­³æ (СÓÐÃûÆø)


¡ï
Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
ÎÒ²ÉÓÃTCA-DOCµÄ·½·¨Å¨ËõÁËһϰûÍâÉÏÇåÒºÖеĵ°°×£¬¿ÉÒÔ¿´¼ûÃ÷ÏԵİ×É«³Áµí£¬µ«ÊǼÓÈëloading bufferÖó·ÐÎå·ÖÖӺ󣬳ÁµíûÓÐÈܽ⣬Ӧ¸ÃÔõô°ì£¿
13Â¥2013-03-29 15:10:30
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ chunqizzm µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼²©] ºþÄÏ´óѧÁõÇÉÁá¿ÎÌâ×é2026ÄêµÚ¶þÅú´Î²©Ê¿Ñо¿ÉúÕÐÉúÐÅÏ¢ +3 ÄÏ·ç¹Û»ð 2026-04-18 3/150 2026-04-19 21:44 by µ­ÑÅÈËÉú27
[¿¼ÑÐ] 304Çóµ÷¼Á +8 castLight 2026-04-16 8/400 2026-04-19 17:14 by ÖÐÔ¥ÄÐ
[¿¼ÑÐ] Çóµ÷¼ÁÍÆ¼ö +9 СÄô°®Ñ§Ï° 2026-04-14 9/450 2026-04-19 17:03 by ÖÐÔ¥ÄÐ
[¿¼ÑÐ] Çóµ÷¼Á +10 СÄô°®Ñ§Ï° 2026-04-16 12/600 2026-04-19 16:51 by ÖÐÔ¥ÄÐ
[¿¼ÑÐ] 085404 22408 309·ÖÇóµ÷¼Á +10 lzmk 2026-04-14 11/550 2026-04-19 16:42 by ÖÐÔ¥ÄÐ
[ÂÛÎÄͶ¸å] ÓÐûÓнÓÊձȽϿìµÄsciÆÚ¿¯Ñ½£¬×îºÃÔÚÒ»¸öÔÂÖ®Äڵģ¬ÑÐÈýº¢×ÓÇó±ÏÒµ 20+4 Ö®»¤×Å 2026-04-16 6/300 2026-04-19 13:00 by Aaron_zyn
[¿¼ÑÐ] 0854Çóµ÷¼Á +23 ÃÅ·ÃþÃþ 2026-04-15 27/1350 2026-04-19 01:59 by ÑÌÓêÁ÷ÑÄ
[¿¼ÑÐ] Ò»Ö¾Ô¸»¦9£¬326ÇóÉúÎïѧµ÷¼Á +12 Áõīī 2026-04-13 12/600 2026-04-18 23:31 by ·²¡Çé
[¿¼ÑÐ] ÉúÎïѧµ÷¼Á +10 Ö½ÉÈzhishan 2026-04-13 10/500 2026-04-18 21:19 by zqndavala
[¿¼ÑÐ] 297£¬¹¤¿Æµ÷¼Á? +5 ºÓÄÏũҵ´óѧ-ÄÜ 2026-04-14 5/250 2026-04-18 15:17 by Equinoxhua
[¿¼ÑÐ] ÊÕµ½¸´ÊÔµ÷¼Áµ«ÊÇÈ¥²»ÁË +8 СÎÏÅ£* 2026-04-16 8/400 2026-04-18 11:15 by zixin2025
[¿¼ÑÐ] 260Çóµ÷¼Á +4 Zyt1314520.. 2026-04-17 5/250 2026-04-18 08:28 by babysonlkd
[¿¼²©] É격/¿¼²© +3 ¿ÐÃæ°üµÄСÊ鳿 2026-04-17 4/200 2026-04-17 23:54 by ÑôÑôÑô^_^
[¿¼ÑÐ] Ò»Ö¾Ô¸»ªÖÐũҵ071010£¬320Çóµ÷¼Á +17 À§À§À§À§À¤À¤ 2026-04-14 19/950 2026-04-17 20:08 by ¹ØÒ»ÕµµÆcd
[»ù½ðÉêÇë] RY£ºÖйú²ú³öµÄ¿ÆÑ§À¬»øÂÛÎÄ£¬¾ø¶ÔÊýÁ¿ºÍ±ÈÀý¶¼ÊÀ½çµÚÒ» +7 zju2000 2026-04-14 18/900 2026-04-16 11:36 by »¶ÀÖËÌÒ¶Ýè
[¿¼ÑÐ] Ò»Ö¾Ô¸AÇø211£¬22408 321Çóµ÷¼Á +6 ËæÐÄËùÓû¡î 2026-04-15 7/350 2026-04-15 21:45 by lbsjt
[¿¼ÑÐ] 297¹¤¿Æµ÷¼Á? +14 ºÓÄÏũҵ´óѧ-ÄÜ 2026-04-13 15/750 2026-04-15 13:25 by ºÚ¿Æ¼¼¿óÒµ
[¿¼ÑÐ] ¿¼Ñе÷¼Á +13 ³¤¹­°Á 2026-04-13 14/700 2026-04-14 14:44 by zs92450
[¿¼ÑÐ] 085408¹âµçÐÅÏ¢¹¤³Ìר˶355Ò»Ö¾Ô¸³¤´º¹â»úËùµ÷¼Á +6 Íõymaa 2026-04-13 13/650 2026-04-14 11:33 by Íõymaa
[¿¼ÑÐ] 245Çóµ÷¼Á +6 ±ùÌÇéÙ?ÆûË® 2026-04-13 10/500 2026-04-14 10:49 by jyl0317
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û