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As mentioned in Section 6.3, 1-MCP treatment of apples, cantaloupes, and other climacteric fruits strongly suppresses biosynthesis of aroma volatiles, particularly esters, at the level of gene transcription and translation (Defilippi et al., 2005b; El-Sharkawy et al., 2005). Kondo et al. (2005) found that production of volatile alcohols and esters remained very low in 1-MCP-treated ‘Delicious’ and ‘Golden Delicious’ apples stored for up to 28 days at 20 °C. A low-dose 1-MCP treatment (0.2 μL L–1) reduced synthesis of acetate esters in ‘Packham’s Triumph’ pears ripened five days at 20 °C after two months of RA storage, but the ester biosynthetic capacity was recovered after four to six months of storage, and a consumer panel preferred 1-MCP-treated over untreated fruit stored for the longer durations (Moya-LeÌn et al., 2006). Similar results were obtained with multiple very low-dose 1-MCP treatments (50 nL L–1) of ‘Conference’ pears (Rizzolo et al., 2005). Most notably, optimal sensory quality was reached after longer storage times and production of butanol and ethyl butanoate were suppressed. Mattheis et al. (2005) evaluated the effects of both 1-MCP and CA, in combination or separately, on the production of various classes of aroma volatiles by ‘Gala’ apples after up to 28 weeks in 1 °C storage plus one week at 20 °C. With or without 1-MCP treatment, ester synthesis declined continuously in CA-stored fruit, whereas apples treated with 1-MCP and stored in air began to produce all types of volatiles after 20–28 weeks. Exposure to ethylene for one week at 20 °C after storage for 12 or 28 weeks stimulated production of mainly esters and alcohols in both CA-stored and 1-MCP treated fruit, but did not increase the levels of aldehydes or acetic acid. Treatment of apples and pears with 1-MCP also inhibited production of the sesquiterpene α-farnesene during cold storage by blocking expression of the α- farnesene synthase gene AFS1 (apple) or PcAFS1 (pear) (Pechous et al., 2005; Gapper et al., 2006). |
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