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bigbangrex

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huangwensh

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bigbangrex(½ð±Ò+5): 2010-11-29 10:04:50
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bigbangrex

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Originally posted by huangwensh at 2010-11-28 21:08:57:
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huangwensh

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bigbangrex(½ð±Ò+1): 2011-04-26 10:22:36
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398279Â¥: Originally posted by huangwensh at 2010-11-30 09:28:32
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huangwensh

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1988604Â¥: Originally posted by 798250242 at 2013-03-28 18:57:42
ÇëÎÊ£¬×ªÄ¤ÒºµÄÅä·½£¬ÎÒÔÚ×öÒ»¸ö260kbµÄ´ó·Ö×Ó£¬×öÁ˼¸´Îwestern blottingû×ö³ö½á¹û£¬ÎÒÃÇʵÑéÊÒµÄתĤҺû¼Ósds£¬½ñÌì¿´µ½ÄúµÄÌù£¬ÏëѧϰÏÂÄãÃǵÄתĤҺÅä·¨£¬ÄÜ·ÖÏíÏÂÂ𣿷¢ÎÒµÄÓÊÏäÒ²ÐУ¬798250242...

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robustli

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wet transfer glycine 14.4g, trisbase 3.03g add water to 800ml the add 200ml methanol. i never transfer 7.5kd protein, but i think you need short transfer time like 25min 75v, for large protein i have done 400kd, 80v 50min. very successful
10Â¥2013-03-30 10:00:16
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