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1) Reviewer 1 placed some interesting questions regarding the filtration/spiking of the samples and the matrix effect. If I agree with the authors regarding to the filtration, that is a standard procedure. Independently of the PAHs adsortion to particulate matter, normally what is measured is the soluble phase. Another type of methodology should be employed considering particulate matter or other phase, what is not the focus of the present manuscript. However, Reviewer 1 has a point regarding matrix effect. It is very typical in new methodologies to show that there is no significant difference between an external calibration or a standard addition method. In other cases, and very usual for PAHs analysis, internal standards may also be employed to overcome this matrix effects. But this is not theory that is checked against the literature but actually measured. When the authors present a method for water analysis, it includes all type of water samples even wastewater, correct? Wastewater can be a very complex matrix.

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[ Last edited by ×Ô˽µÄè1988 on 2014-12-9 at 15:16 ]
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