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Romaner007

金虫 (小有名气)

应助: 4 (幼儿园)
金币: 2692
帖子: 170
在线: 44.3小时
虫号: 2117057
注册: 2012-11-09
性别: GG
专业: 细胞增殖、生长与分化

[求助] 关于甲基化PCR设计引物的问题，做这块的速来围观，给点指导！

TATAACATTTAACCCTGGTCAGGTTGCTAGGTCATATATTTTGTGTTTCCTTTCTCGGCCGGCCTGCGGCGGCGGCAGCGGCGGCGTTTCTCGCCTCCTCTTCGTCTTTTCTAACCGTGCAGCCTCTTCCTAGGCTTCTCCTGAAAGGGAAGGTGGAAGCCGTGGGCTCGGGCGGGAGCCGGCTGAGGCGCGGCGGCGGCGGCGGCACCTCCCGCTCCTGGAGCGGGGGGGAGAAGCGGCGGCGGCGGCGGCCGCGGCGGCTGCAGCTCCAGGGAGGGGGTCTGAGTCGCCTGTCACCATTTCCAGGGCTGGGAACGCCGGAGAGTTGGTCTCTCCCCTTCTACTGCCTCCAACACGGCGGCGGCGGCGGCTGGCACATCCAGGGACCCGGGCCGGTTTTAAACCTCCCGTGCGCCGCCGCCGCACCCCCCGTGGCCCGGGCTCCGGAGGCCGCCGGCGGAGGCAGCCGTTCGGAGGATTATTCGTCTTCTCCCCATTCCGCTGAAGCTGCTGCCAGGCCTCTGGCTGCTGAGGAGAAGCAGGCCCAGTCGCTGCAACCATCCAGCAGCC
Blue CG is a potential site of mythelation in promotor region of gene X101. When the promotor DNA was treated with bisulfite, please design primers that should be as described below:
1. Confirm the site methylated or unmethylated with site-specific PCR.
2. PCR result is positive when the Blue CG is methylated.
3. PCR result is positive when the Blue CG is unmethylated.
4. PCR product will be 400 bp at length on agarose gel.
5. 20-21 bp of primers will be synthesized commercially.

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