[求助] 【求组】有谁熟悉二代测序吗？

2楼: Originally posted by wizardfan at 2013-09-18 07:22:11
你用的是Trinity？FPKM有什么意义我到现在也不知道，如果你知道的话，请解释一下。

3楼: Originally posted by 我要学perl么 at 2013-09-18 12:00:11
用的是Tophat和Cufflinks。

对于FPKM我是百度百科的。。
我的理解有点肤浅，觉得它在一定程度上反映了feature与总features量上的比值，也就是说反映表达量吧。

刚刚有人这么说：“RPKM是一个经过归一化的数 ...

4楼: Originally posted by wizardfan at 2013-09-26 00:23:57
RPKM (Reads Per Kilo base per Million mapped reads) was calculated to represent the expression level for each transcript.
Mortazavi, A.; Williams, B. A.; McCue, K.; Schaeffer, L.; Wold,
B. Mapping ...

4楼: Originally posted by wizardfan at 2013-09-25 17:23:57
RPKM (Reads Per Kilo base per Million mapped reads) was calculated to represent the expression level for each transcript.
Mortazavi, A.; Williams, B. A.; McCue, K.; Schaeffer, L.; Wold,
B. Mapping ...