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毛桃就是我

银虫 (著名写手)

[求助] 关于提交ITS序列到genbank

通过bankit把序列提交了 然后收到回信 要求增加信息  其中有一条是让说明序列的详细信息  但是我不清楚 做这个ITS也单纯是为了鉴定菌株 这个需要怎么回复呀?
以下是回信摘录
[4] Complete feature annotation has not been included for some or all of
the sequence(s) you have submitted.

  A. Please resubmit your sequence(s) with relevant features such as:

- coding regions (CDS features), partial or complete, including
  nucleotide spans and reading frame. Using this information,
  our software will add the amino acid translations for you.
- structural RNAs such as rRNAs, tRNAs, misc_RNAs, with
  nucleotide spans
- features which may describe your sequence, such as
  repeat_regions, UTRs, promoters with nucleotide spans

     We have appended instructions for adding features to records. We
     cannot accept annotation in text format, you must resubmit.

     If your annotated sequences have coding region features with
     internal stop codons or reading frameshifts, you will be asked
     to review and resubmit. Please be sure to analyze your data prior
     to resubmission.

  B. If you know the correct annotation, but are unsure how to add it,
     please contact GenBank Users Services at: info@ncbi.nlm.nih.gov
     and include this message with your request.

  C. If you are unable to determine the feature annotation, please
     provide a biological reason for the lack of annotation.

[ Last edited by 毛桃就是我 on 2013-6-21 at 11:26 ]
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毛桃就是我

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引用回帖:
2楼: Originally posted by ideaxum at 2013-09-13 10:43:29
楼主,你的问题解决没有啊?我的测序结果让标明5.8sRNA,18sRNA的位置,不知道怎么弄啊

注明位置不知道 就可以了
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3楼2013-09-16 15:20:44
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ideaxum

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楼主,你的问题解决没有啊?我的测序结果让标明5.8sRNA,18sRNA的位置,不知道怎么弄啊
2楼2013-09-13 10:43:29
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秦qin

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引用回帖:
2楼: Originally posted by ideaxum at 2013-09-13 10:43:29
楼主,你的问题解决没有啊?我的测序结果让标明5.8sRNA,18sRNA的位置,不知道怎么弄啊

楼主  想问一下你上传序列到Features (Overview) 这一步是怎么做的
4楼2018-04-11 19:41:18
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snoopytbw

荣誉版主 (著名写手)

你测序结果blast,是非编码区么,如果是核糖体的话就标记为rRNA就好了哇,好像要求按照格式重新编辑好fasta发邮件到指定邮箱的吧,你仔细看看要求

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5楼2018-04-11 20:30:43
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