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shengwuhj

金虫 (初入文坛)

[求助] real time prc

最近投了一篇文章,其中一个实验是有qPCR做的,绝对定量,但是审稿人问了一个问题不知道如何回答:there is no control for the PCR itself; hence, the overabundance of A gene over B gene could be due to PCR or primer bias, a more efficient PCR reaction with A gene primers, or a number of other experimental issues unrelated to gene abundance. 问题是做绝对定量的时候都用质粒做了标准曲线的,所以应该说扩增效率并不影响实验结果吧(除非扩增效率过高或者过低)。对qPCR不是特别懂,望大神指点啊。
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这个问题更适合去相关专业版求助
2楼2013-04-07 14:40:34
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