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luoho100

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Sample TextSample TextRpv10: a new locus from the Asian Vitis gene pool for pyramiding
downy mildew resistance loci in grapevine


grapevine A population derived from a breeding  strain  Cf.Ca-52-42
cross between and  cultivar`Solaris' consisting of 265 F1-individuals was genetically mapped using SSR markers and screened for downy mildew resistance.  Quantitative trait locus (QTL) analysis
revealed two strong QTLs on linkage groups (LCs) 18 and
09. The locus on LC 18 was found to be identical with the
previously described locus Rpv3 and is transmitted by
Cf.Ca-52-42.  `Solaris' transmitted the resistance-related
locus on LC 09 explaining up to 50% of the phenotypic
variation in the population. This downy mildew resistance
locus is named Rpv10 for resistance to Pla.smopara viticola. Rpv10 was initially introgressed from Viti.s amuren.si.s,  a wild species of the Asian Viti.s gene pool. The one-LOD supported confidence interval of the QTL spans a section of 2.1 centi Morgan (cM) corresponding to 314 kb
in the reference genome PN40024 (12x). Eight resistance
gene analogues (RCAs) of the NBS-LRR type and addi-
tional resistance-linked genes are located in this region of
PN40024. The F1 sub-population which contains the Rpv3
as well as the Rpv10 locus showed a significantly higher
degree of resistance, indicating additive effects by pyramiding  of  resistance  loci.  Possibilities  for  using  the resistance locus Rpv10 in a grapevine breeding programme  are  discussed.  Furthermore,  the  marker  data  revealed
`Severnyi' x `Muscat Ottonel' as the true parentage for the
male parent of `Solaris'.
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luoho100

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