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Sample TextSample TextRpv10: a new locus from the Asian Vitis gene pool for pyramiding downy mildew resistance loci in grapevine grapevine A population derived from a breeding strain Cf.Ca-52-42 cross between and cultivar`Solaris' consisting of 265 F1-individuals was genetically mapped using SSR markers and screened for downy mildew resistance. Quantitative trait locus (QTL) analysis revealed two strong QTLs on linkage groups (LCs) 18 and 09. The locus on LC 18 was found to be identical with the previously described locus Rpv3 and is transmitted by Cf.Ca-52-42. `Solaris' transmitted the resistance-related locus on LC 09 explaining up to 50% of the phenotypic variation in the population. This downy mildew resistance locus is named Rpv10 for resistance to Pla.smopara viticola. Rpv10 was initially introgressed from Viti.s amuren.si.s, a wild species of the Asian Viti.s gene pool. The one-LOD supported confidence interval of the QTL spans a section of 2.1 centi Morgan (cM) corresponding to 314 kb in the reference genome PN40024 (12x). Eight resistance gene analogues (RCAs) of the NBS-LRR type and addi- tional resistance-linked genes are located in this region of PN40024. The F1 sub-population which contains the Rpv3 as well as the Rpv10 locus showed a significantly higher degree of resistance, indicating additive effects by pyramiding of resistance loci. Possibilities for using the resistance locus Rpv10 in a grapevine breeding programme are discussed. Furthermore, the marker data revealed `Severnyi' x `Muscat Ottonel' as the true parentage for the male parent of `Solaris'. µÈ´ýÖÐ ¼±Ðè´ð°¸ÎÒ Ð»Ð» |
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wyc110112
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