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Cathode Buffer(5x):         0.5M Tris, 0.5M Tricine Buffer     0.5% SDS, Ph8.25
Gel Buffer    (3x):        3M Tris-Cl, 0.3% SDS, pH=8.45
Glycerol :                  50%
GelSolution(49.5%T,6%C): 46.5g acrlamide,3,N¡¯-Methylene bisacrylamide   add water to 100 ml   
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Separating Gel (16.5%)
Gel solution: 3 ml
Gel Buffer  : 3 ml
50% Glycerol: 3 ml
10% AP      : 100 ul
TEMED       : 10 ul


Stacking Gel  ( 4% )
Gel solution: 0.5 ml
Gel Buffer  : 2.1 ml
Water       : 3.6 ml
10% AP      : 70 ul
TEMED        : 8 ul


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