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Gel Buffer    (3x):        3M Tris-Cl, 0.3% SDS, pH=8.45
Glycerol :                  50%
GelSolution(49.5%T,6%C): 46.5g acrlamide,3,N¡¯-Methylene bisacrylamide   add water to 100 ml   
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TEMED       : 10 ul


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Gel Buffer  : 2.1 ml
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10% AP      : 70 ul
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