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chaoen

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Dear all,

Recently I found one of my mutants contains stop codon before 100 aminoacid to the end. When I checked the expression by SDS-page, and found there are two equally dense bands (one supposed to be the partial protein and the other is the full protein).
The stop codon for my case is TAG. I know it is reasonable it can be bypassed with stopcodon.
Now I am wondering which amino acid will be translated to in case of the stop codon readthrough? and Which strategy can be applied to make the stop codon efficiently to stop expression fully at that position?
Thanks in advance!

Any suggestion is welcome!
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chaoen

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2Â¥2011-08-31 20:40:06
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jtliuok

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Add two or three stop codons will be ok.
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3Â¥2011-10-12 20:29:04
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