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Alfalfa (Medicago sativa), a fodder crop for livestocks, is characterized by its high protein, high energy and high resistance to drought. However, in spite of the high protein content, the composition of the amino acids in alfalfa protein is not balanced for the low content in sulphur-containing amino acids, namely methionine and cysteine which are two essential amino acids. In this case, the existing alfalfa varieties as a single source of forage will result in the decline in the quality of livestocks. Up to now, the attempts to enhance the content of sulphur-containing amino acids have been mainly focused on the engineering modification of the methionine synthetase, little attention has been paid to the initiation enzyme for the synthesis of sulphur-containing amino acids, the systeine synthetase, and its relationship to methionine. For this reason, this study will be focusing on the cloning and sequencing of the genes for the systeine synthetase, so as to provide convenience for the cloning of the genes for the isozymes of the systeine synthetase in alfalfa plants.
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